Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   VITFI_RS15570 Genome accession   NZ_CP022423
Coordinates   3304265..3305503 (+) Length   412 a.a.
NCBI ID   WP_089417761.1    Uniprot ID   -
Organism   Vitreoscilla filiformis strain ATCC 15551     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3299265..3310503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VITFI_RS15545 (VITFI_CDS3114) rpmA 3300419..3300676 (-) 258 WP_089417757.1 50S ribosomal protein L27 -
  VITFI_RS15550 (VITFI_CDS3115) rplU 3300710..3301021 (-) 312 WP_089417758.1 50S ribosomal protein L21 -
  VITFI_RS15555 (VITFI_CDS3116) - 3301279..3302220 (+) 942 WP_089417759.1 polyprenyl synthetase family protein -
  VITFI_RS15565 (VITFI_CDS3117) pilF 3302503..3304236 (+) 1734 WP_089417760.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VITFI_RS15570 (VITFI_CDS3118) pilG 3304265..3305503 (+) 1239 WP_089417761.1 type II secretion system F family protein Machinery gene
  VITFI_RS15575 (VITFI_CDS3119) - 3305503..3306438 (+) 936 WP_089417762.1 A24 family peptidase -
  VITFI_RS15580 (VITFI_CDS3120) coaE 3306435..3307064 (+) 630 WP_089417763.1 dephospho-CoA kinase -
  VITFI_RS15585 (VITFI_CDS3121) zapD 3307105..3307860 (+) 756 WP_089417764.1 cell division protein ZapD -
  VITFI_RS15590 - 3307911..3308129 (+) 219 WP_089417765.1 DNA gyrase inhibitor YacG -
  VITFI_RS15595 (VITFI_CDS3123) - 3308304..3309587 (+) 1284 WP_089417766.1 hypothetical protein -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44486.96 Da        Isoelectric Point: 9.6231

>NTDB_id=239295 VITFI_RS15570 WP_089417761.1 3304265..3305503(+) (pilG) [Vitreoscilla filiformis strain ATCC 15551]
MATASKPTADKKPTPEQTYDWEGKDRSGKAVKGEIRAAGEAAASANLRRQGIMVSKIKKRRTSTFGAPSITTKDIAVFTR
QLSTMMKAGVPLMQSFDIVAKGSANATLTRLLLDIKADIESGTSMSGAFRRYPQYFDNLYCNLVEAGEAGGILEQLLNRL
AIYQEKSQALKQRVKSAMTYPISVMVVAGGVVTVIMIFVIPQFEEVFKGFGAELPGPTQLVINMSRFFVANWYLIFGGLA
LFGYTVKESFKRSVALQQALDRVKLKLPVFGDLIQKSIVARWTRTLATLFAAGVPLVDALESVGGTAGNSVYTIATAKIQ
REVSTGTGLTTAMTASGIFPNMVLQMCSIGEESGALDDMLNKAAEFFEEEVDEMVKGLSSLMEPIIIVFLGTIIGGIVVA
MYLPIFKMGGAV

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=239295 VITFI_RS15570 WP_089417761.1 3304265..3305503(+) (pilG) [Vitreoscilla filiformis strain ATCC 15551]
ATGGCCACAGCCTCTAAACCGACAGCGGACAAAAAGCCCACGCCCGAACAAACCTACGACTGGGAAGGCAAAGATCGCAG
CGGCAAAGCCGTTAAAGGGGAGATACGCGCTGCTGGCGAAGCGGCTGCCAGTGCCAATCTGCGTCGCCAGGGCATCATGG
TCAGCAAGATCAAGAAGCGCCGCACGTCGACGTTTGGTGCCCCGTCCATCACCACCAAGGACATCGCTGTTTTCACGCGG
CAACTGTCCACCATGATGAAAGCCGGTGTGCCGCTGATGCAGTCTTTTGACATCGTGGCGAAGGGCAGTGCGAACGCGAC
GCTAACACGCTTGCTGCTGGACATCAAAGCCGATATCGAAAGTGGCACCAGCATGTCGGGTGCCTTTCGCCGCTATCCGC
AATATTTTGACAACCTTTACTGCAACTTGGTCGAAGCAGGTGAGGCAGGCGGCATTTTGGAACAACTGCTGAACCGGTTG
GCAATTTATCAAGAAAAAAGCCAAGCTCTGAAACAACGCGTCAAGTCAGCCATGACCTACCCGATTTCGGTGATGGTGGT
CGCGGGGGGGGTGGTGACGGTGATCATGATCTTTGTGATCCCCCAATTCGAAGAAGTTTTCAAGGGCTTTGGTGCCGAAC
TCCCCGGCCCGACTCAGTTGGTCATCAACATGAGTCGATTTTTCGTGGCCAACTGGTACCTGATTTTTGGCGGTTTGGCT
CTTTTTGGTTATACCGTCAAAGAATCTTTTAAGCGTTCAGTCGCTTTGCAGCAGGCCCTGGATCGTGTCAAATTGAAACT
GCCTGTTTTTGGAGATTTGATTCAAAAATCCATCGTTGCGCGTTGGACTCGCACTTTGGCCACGCTGTTTGCAGCGGGGG
TTCCTTTGGTCGATGCACTCGAATCGGTGGGGGGAACGGCGGGTAATTCGGTCTATACCATCGCCACCGCTAAGATCCAG
CGCGAAGTTTCCACTGGCACAGGCTTGACGACGGCCATGACCGCCTCAGGCATTTTCCCCAACATGGTGCTGCAAATGTG
CTCGATCGGCGAGGAATCGGGCGCGTTGGATGACATGCTCAACAAAGCCGCCGAGTTTTTTGAGGAAGAAGTCGATGAGA
TGGTCAAAGGGCTGTCTTCCCTGATGGAGCCCATCATCATCGTTTTTCTGGGCACCATCATTGGTGGGATTGTGGTGGCC
ATGTACCTGCCCATCTTCAAAATGGGTGGCGCTGTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)

Type II secretion system (T2SS), protein F (T2SSF)

(78-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

52.5

97.087

0.51
  pilG Neisseria meningitidis 44/76-A

51.759

96.602

0.5
  pilC Pseudomonas stutzeri DSM 10701

50.372

97.816

0.493
  pilC Acinetobacter baylyi ADP1

49.633

99.272

0.493
  pilC Legionella pneumophila strain ERS1305867

50.123

98.301

0.493
  pilC Acinetobacter baumannii D1279779

49.386

98.786

0.488
  pilC Vibrio cholerae strain A1552

42.211

96.602

0.408
  pilC Vibrio campbellii strain DS40M4

41.162

96.117

0.396
  pilC Thermus thermophilus HB27

37.709

100

0.383

Multiple sequence alignment