Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   VITFI_RS15565 Genome accession   NZ_CP022423
Coordinates   3302503..3304236 (+) Length   577 a.a.
NCBI ID   WP_089417760.1    Uniprot ID   -
Organism   Vitreoscilla filiformis strain ATCC 15551     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3297503..3309236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VITFI_RS15530 (VITFI_CDS3111) - 3297505..3298143 (+) 639 WP_089417754.1 RNA pyrophosphohydrolase -
  VITFI_RS15535 (VITFI_CDS3112) proB 3298112..3299230 (-) 1119 WP_089417755.1 glutamate 5-kinase -
  VITFI_RS15540 (VITFI_CDS3113) cgtA 3299227..3300312 (-) 1086 WP_089417756.1 Obg family GTPase CgtA -
  VITFI_RS15545 (VITFI_CDS3114) rpmA 3300419..3300676 (-) 258 WP_089417757.1 50S ribosomal protein L27 -
  VITFI_RS15550 (VITFI_CDS3115) rplU 3300710..3301021 (-) 312 WP_089417758.1 50S ribosomal protein L21 -
  VITFI_RS15555 (VITFI_CDS3116) - 3301279..3302220 (+) 942 WP_089417759.1 polyprenyl synthetase family protein -
  VITFI_RS15565 (VITFI_CDS3117) pilF 3302503..3304236 (+) 1734 WP_089417760.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VITFI_RS15570 (VITFI_CDS3118) pilG 3304265..3305503 (+) 1239 WP_089417761.1 type II secretion system F family protein Machinery gene
  VITFI_RS15575 (VITFI_CDS3119) - 3305503..3306438 (+) 936 WP_089417762.1 A24 family peptidase -
  VITFI_RS15580 (VITFI_CDS3120) coaE 3306435..3307064 (+) 630 WP_089417763.1 dephospho-CoA kinase -
  VITFI_RS15585 (VITFI_CDS3121) zapD 3307105..3307860 (+) 756 WP_089417764.1 cell division protein ZapD -
  VITFI_RS15590 - 3307911..3308129 (+) 219 WP_089417765.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 63260.95 Da        Isoelectric Point: 5.9316

>NTDB_id=239294 VITFI_RS15565 WP_089417760.1 3302503..3304236(+) (pilF) [Vitreoscilla filiformis strain ATCC 15551]
MDTVVETPSPSPVALTGVARVLVTRGKLTAKRAEELLSTSREKKTSFVNILLAAGDVSPRVLAHTLSMALAIPLLDLSAV
DTKQLPRSLLDRKLIETYRVYPLSKRGSRLFVACADPTDQEALERVKFATQLTPEWILVEADKLQRLIASESISNNEALE
STGGYDFDFELTDAEKSSAEDPVGDLAGDTEDAPVVKFLQRMLIDAIDMRASDLHFEPYEYNYRVRFRVDGELREIVQPP
VAIKDKLASRIKVVSRLDIAEKRVPQDGRMKLRVGNRTIDFRVSSLPTLFGEKIVIRILDSSNAKLGIEALGYEPEEKAR
LLTAVQRPYGMVLVTGPTGSGKTVSLYTCLNILNQPGVNLCTVEDPAEINMPGINQVNVNDKAGLTFSSALKSFLRQDPD
VIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPATVTRLLNMGVAPFNVASSLLLITAQRLVRRLCANCKQPIDIPR
QALLDAGFTEDEVDGTWKPYKPVGCEACNKGFRGRLGIYQAMPISEPIQRLILASGTVMEIAAQARKDGVRDLRQSGLLK
VKLGMTSLEEVLAATNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=239294 VITFI_RS15565 WP_089417760.1 3302503..3304236(+) (pilF) [Vitreoscilla filiformis strain ATCC 15551]
GTGGATACCGTTGTTGAAACGCCCTCTCCTTCCCCTGTTGCACTCACGGGGGTCGCCCGCGTCTTGGTCACCCGAGGCAA
GCTCACGGCCAAACGCGCTGAGGAGTTGCTCAGCACATCACGCGAAAAGAAAACCTCGTTCGTGAACATCTTGCTGGCTG
CGGGCGATGTTTCGCCGCGCGTGCTGGCTCATACGTTGTCGATGGCGTTGGCCATCCCCCTGCTCGATTTGAGTGCGGTC
GATACCAAACAACTGCCCCGCAGCTTGCTGGATCGAAAACTCATTGAAACCTACCGGGTTTATCCGTTGAGCAAACGGGG
CAGCCGTTTGTTTGTGGCGTGCGCAGATCCCACAGATCAAGAAGCGCTGGAACGGGTGAAGTTTGCCACCCAGCTCACGC
CTGAATGGATTTTGGTTGAGGCCGACAAACTGCAACGCCTCATTGCCTCAGAAAGCATCAGCAACAATGAGGCGCTCGAA
TCCACGGGCGGTTACGACTTTGACTTTGAACTGACCGATGCAGAAAAGTCCAGTGCCGAAGACCCCGTCGGAGATCTCGC
CGGAGACACAGAAGACGCGCCGGTGGTGAAGTTTTTACAACGCATGCTGATCGATGCGATTGACATGCGGGCGTCGGACT
TGCACTTTGAACCCTACGAGTACAACTACCGGGTGCGCTTCCGGGTGGATGGGGAGTTGAGAGAAATCGTTCAACCCCCG
GTAGCCATCAAAGACAAATTAGCCTCTCGCATCAAAGTGGTTTCTCGGCTGGACATCGCAGAAAAGCGCGTCCCCCAAGA
TGGCCGGATGAAACTGCGCGTGGGCAACCGAACGATCGATTTCCGCGTCAGCTCACTGCCGACCTTGTTTGGTGAAAAGA
TCGTGATCCGTATTTTGGATTCGTCCAATGCCAAACTGGGCATCGAGGCCCTCGGGTACGAGCCTGAAGAGAAGGCGCGT
CTCCTGACAGCAGTTCAGCGCCCTTACGGCATGGTGCTGGTCACAGGGCCGACCGGTAGCGGGAAAACGGTGTCACTCTA
CACCTGCTTGAACATTCTGAATCAACCCGGCGTTAACCTTTGTACTGTAGAAGACCCAGCAGAAATTAACATGCCGGGCA
TCAATCAAGTGAACGTGAATGACAAAGCGGGTTTGACGTTCTCCAGCGCACTCAAATCTTTCCTGCGTCAGGATCCGGAT
GTCATCATGGTGGGTGAAATTCGGGACTTGGAAACCGCCGATATCGCCATCAAAGCCGCTCAAACAGGCCACTTGGTGCT
GTCCACCTTGCACACCAACGATGCGCCTGCCACAGTGACCCGGCTGCTCAACATGGGGGTCGCTCCTTTCAACGTCGCCT
CCAGCCTGCTGTTGATCACTGCCCAACGGCTGGTTCGCCGCTTGTGCGCAAACTGCAAACAACCCATCGACATCCCGCGC
CAGGCACTGCTCGATGCTGGTTTTACCGAAGACGAGGTGGACGGCACCTGGAAACCGTACAAACCCGTGGGATGCGAAGC
CTGTAACAAAGGATTCCGGGGACGTTTGGGGATTTATCAGGCCATGCCTATTTCGGAACCCATCCAGCGTCTGATTCTTG
CCAGTGGCACCGTCATGGAAATTGCGGCGCAAGCGCGCAAAGACGGGGTGCGAGATTTGCGGCAATCCGGCCTTCTGAAA
GTCAAACTCGGCATGACCTCGCTGGAGGAAGTGCTCGCCGCCACCAACGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(202-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

52.197

98.614

0.515
  pilB Acinetobacter baumannii D1279779

52.381

98.267

0.515
  pilB Acinetobacter baylyi ADP1

52.021

98.614

0.513
  pilB Legionella pneumophila strain ERS1305867

48.582

97.747

0.475
  pilB Vibrio cholerae strain A1552

47.464

95.667

0.454
  pilB Vibrio parahaemolyticus RIMD 2210633

46.454

97.747

0.454
  pilB Vibrio campbellii strain DS40M4

46.346

97.227

0.451
  pilF Thermus thermophilus HB27

38.146

97.227

0.371
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.212

92.374

0.362

Multiple sequence alignment