Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   CFN77_RS05270 Genome accession   NZ_CP022319
Coordinates   1052941..1056645 (+) Length   1234 a.a.
NCBI ID   WP_096881210.1    Uniprot ID   -
Organism   Bacillus altitudinis strain SGAir0031     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1047941..1061645
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFN77_RS05255 (CFN77_05295) - 1048239..1048649 (+) 411 WP_096881208.1 DoxX family protein -
  CFN77_RS05260 (CFN77_05300) - 1048841..1049287 (-) 447 WP_007499129.1 ferritin-like domain-containing protein -
  CFN77_RS05265 (CFN77_05305) addB 1049454..1052963 (+) 3510 WP_096881209.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  CFN77_RS05270 (CFN77_05310) addA 1052941..1056645 (+) 3705 WP_096881210.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  CFN77_RS05275 (CFN77_05315) sbcD 1056671..1057843 (+) 1173 WP_096881211.1 exonuclease subunit SbcD -
  CFN77_RS05280 (CFN77_05320) - 1057840..1061235 (+) 3396 WP_096881212.1 AAA family ATPase -
  CFN77_RS05285 (CFN77_05325) - 1061253..1061552 (+) 300 WP_017360217.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142611.50 Da        Isoelectric Point: 5.0684

>NTDB_id=238296 CFN77_RS05270 WP_096881210.1 1052941..1056645(+) (addA) [Bacillus altitudinis strain SGAir0031]
MQIPKPNNSTWTDDQWNAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEQPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELSKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPAPEQWMRAFLSLYDVDTQTKVEELPFSPYIKEDLSLVLRSCQELLEQA
LTLSKEPGGPAPRAENFIDDLEQVNELIRHQDDFETLYELLPTINFKRLKTCKGDDYDPALLEKATDARNQAKKQLEKLK
DEYFMRSPDQHLKSLAEMKPVVETLVELVIQYGERFERAKQEKSIVDFSDLEHYCLRILAKQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGKHPAEGNRFMVGDVKQSIYRFRLAEPMLFLNKYKQFKADSKETGKRIDLNKNFR
SRADVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPQSEDTTTEMLLVHLDQHDTESDEEREELETVQFEARVIAKK
IKELVEQPFQVYDAKQQLTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPLVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCRKLERFFLQLRKWRDFSIQHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLIAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAANGDDWLLPDFERYQARTYLDFIGPALIRHQAMSSLLEESREVVLSHPSSFSITFHQASDL
LQEDMSLEKQKQDEVVQALMEGVPVQGYGDYEEEVKERLSWRYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPASIRKAH
ATLYERPAFMKKKTLTAAEQGTAMHTVMQHIPLPSNEPYDESRVEELLHSLKEKDLLTDEQIQSINREGIVAFFSTAIGQ
KLQKADWVKREVSFSMVLPVKEVYSHIDAEGEPVLIQGMIDCLFEADGKLYLLDYKTDRVTGRFSGGLEEAEAMLKKRYE
TQISLYTAAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=238296 CFN77_RS05270 WP_096881210.1 1052941..1056645(+) (addA) [Bacillus altitudinis strain SGAir0031]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACAGATGACCAATGGAACGCCATCGTTTCAGAAGGACAAGATATTCT
TGTTGCTGCGGCGGCAGGGTCAGGGAAAACAGCGGTCTTGGTTGAACGGCTCATTCGAAAAATGACTCGGCCGGAGCAGC
CAGTTGATGTGGACAGGCTGCTCGTAGTGACTTTTACCAATGCTTCAGCAGCAGAAATGAAGCACCGGATCACAGAAGCA
CTTGAAAAAGAACTATCTAAAAACCCTGGTTCCCTTCATATGCGGAGACAGCTGTCACTTATGAATCGTGCCAATATTTC
TACCTTGCACTCCTTTTGTTTGCAAGTTCTGCGCACTTTTTATTATGAGATTGACCTTGATCCAGGTTTTCGTTTGGCTG
ATCAAACAGAAGGAGAATTACTTGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAGTATAAGGCTGGAAAGCCGTCA
TTCTTTGAACTCGTTGATCGTTACACATCTGATCGGCATGATTTAGATCTTCAATGGCTTGTCAAAAGAATTTATGAGTT
TTCTAGATCACATCCAGCGCCAGAACAGTGGATGCGGGCATTCCTTTCTCTTTATGATGTGGATACACAGACAAAGGTAG
AAGAATTACCCTTTTCTCCATATATCAAAGAGGACCTTTCTCTCGTTTTAAGAAGCTGCCAGGAACTGCTGGAACAAGCG
CTCACTTTATCAAAAGAGCCGGGCGGTCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTAAATGAATT
AATAAGGCATCAAGATGATTTTGAGACACTTTATGAGCTTTTACCAACTATCAATTTTAAAAGGTTAAAGACTTGCAAAG
GGGATGACTATGACCCAGCTCTGTTAGAAAAAGCAACAGATGCACGCAATCAAGCCAAAAAACAATTAGAAAAGCTAAAA
GATGAATACTTTATGCGCAGTCCCGACCAGCATTTAAAAAGCTTGGCTGAAATGAAGCCAGTTGTAGAAACACTTGTAGA
GCTTGTGATTCAATATGGAGAGCGTTTCGAAAGAGCCAAACAAGAAAAATCAATCGTAGACTTTTCGGATCTAGAACATT
ATTGTTTGCGCATTTTAGCGAAGCAGGACGCAGAAGGAAATCTCATTGAAACAGAAGCGGCTAAATACTATCAACAGCAA
TTTGAAGAAGTTCTCGTGGATGAATACCAGGATACAAATCTTGTCCAAGAAACGATTTTAAAGCTCGTGTCTAAAGGGAA
ACATCCTGCAGAAGGCAACCGCTTTATGGTTGGTGATGTGAAGCAGTCCATTTATCGCTTTAGACTTGCAGAGCCGATGC
TCTTTTTAAATAAATATAAACAATTCAAAGCAGACAGCAAAGAAACAGGTAAAAGAATTGATCTAAATAAAAACTTCCGC
AGTCGGGCTGATGTATTAGACAGTACCAACTTTCTGTTCAAACAGCTGATGGGAGAAACAGTTGGAGAAATTGAATATGA
CGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCGCAAAGCGAAGACACGACGACAGAAATGCTTCTTGTTCATTTAG
ATCAGCACGACACAGAAAGCGATGAAGAACGAGAAGAGCTAGAGACGGTACAATTTGAGGCGAGAGTGATTGCAAAGAAA
ATAAAAGAATTGGTAGAACAGCCTTTTCAAGTGTACGATGCAAAACAGCAGCTGACACGCAACTTGCAGTATCGAGACAT
TGTGATCTTGCTTCGATCAATGCCGTGGGCTCCGCAAATGATGGAAGAGTTGAAGAAGCAGGGAATACCTGTTTATGCGA
ATCTTTCCTCAGGCTATTTCGAAGCGACAGAGGTATCTGTCATTCTTTCTTTGTTAAAAGTGATTGATAATCCATATCAA
GATATTCCGCTCGCTGCTGTTTTAAGGTCACCTCTTGTTCATTTAGACGAAAATGAGATGGCACTGATTAGAACCAGTGA
TAAAAAAGGCACTTATTATGACGCTGTGAAGGCATTTATGAGTGTGACACACTCTGATCATCCGACATGCAGAAAGCTGG
AGCGATTCTTCCTTCAATTACGCAAATGGCGTGATTTTTCGATTCAGCATTCGGTAGCAGAACTGATTTGGGAAGTGTAC
CGAGATACACAATATCTAGATTATGTCGGCGGGATGCCAGGAGGTAAGCAAAGACAGGCAAACCTTCGGGCTTTATATGA
TCGAGCGAAACAGTATGAAAAGGCTGCATTTAGAGGACTGTTTCGATTTCTTCGATTTATTGAAAGAATGCAAGAGCGTG
GAGATGATCTTGGAGCGGCAAAAACGTTCAGTGAAACAGAGGATGTTGTCCGCATGATGACCATTCATAGCAGTAAAGGA
CTCGAATTCCCTGTTGTATTTACTGCAGGACTTGGCAGGAACTTTAACATGATGGATTTAAACCAGTCCTATTTGCTCGA
CAAAGAATTAGGCTTTGGCAGCAAATATATTCATCCTGAGCTGAGAATTAGTTATGCAACATTACCGCTGATCGCGATGA
AAAAAAAGATGAGAAAAGAGTTATTATCTGAAGAACTGAGGGTTCTATATGTGGCGCTCACAAGGGCGAAGGAAAAATTA
TTTTTAGTCGGCTCTGTCAAAAATCAAGTGAAAGCTTTAAGCAAGTGGCAAAATGCCGCAAACGGAGACGACTGGCTCCT
CCCTGATTTTGAACGTTATCAAGCGAGAACATACTTAGATTTTATTGGCCCAGCCCTTATCCGTCATCAAGCGATGTCTT
CTCTTTTAGAAGAATCGAGAGAAGTCGTTCTCTCGCATCCATCATCATTTTCTATCACCTTTCATCAAGCGTCCGATCTT
CTACAAGAAGACATGTCACTTGAAAAACAAAAACAAGACGAAGTGGTACAAGCATTAATGGAAGGTGTGCCTGTTCAAGG
ATATGGCGATTACGAGGAGGAAGTAAAAGAAAGACTTTCATGGCGTTATCCATATTTAGCGGCCTCCCAAGTAGGGACAA
AGCAGTCAGTTTCCGAGATCAAAAGAATGAAAGAAATACAGGATGAATACAGTGTGCCTGCTTCCATTCGAAAAGCTCAT
GCGACACTGTACGAACGACCGGCTTTTATGAAGAAAAAAACACTCACAGCCGCAGAACAAGGGACGGCCATGCATACAGT
CATGCAGCATATCCCTCTTCCTTCAAATGAACCATATGATGAATCTCGTGTAGAAGAGCTGCTCCATTCTTTAAAAGAGA
AAGACTTACTAACAGATGAACAAATCCAGTCCATCAATCGAGAAGGAATTGTGGCCTTCTTTTCTACTGCTATTGGTCAA
AAACTGCAGAAAGCGGACTGGGTGAAACGAGAAGTATCGTTTAGTATGGTTTTACCGGTTAAAGAGGTATACAGCCATAT
TGATGCAGAAGGTGAACCTGTTCTCATTCAAGGAATGATCGATTGTTTATTTGAAGCAGATGGCAAGCTCTATTTACTTG
ATTATAAAACAGATCGAGTGACTGGCAGATTCTCAGGTGGATTAGAGGAAGCTGAAGCGATGTTAAAAAAACGCTATGAA
ACGCAAATATCGTTATATACAGCAGCGGTAGAACGTTTAACAAACAGAACATTAGAGGAGAAAATCCTTTATTTCTTTGA
TGGAAATCTAGAAATTTCTTTATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-463)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1226)

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.855

100

0.67

Multiple sequence alignment