Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   UN84_RS06965 Genome accession   NZ_CP022134
Coordinates   1465359..1466585 (-) Length   408 a.a.
NCBI ID   WP_046078552.1    Uniprot ID   -
Organism   Halomonas sp. HG01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1460359..1471585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UN84_RS06940 purU 1460920..1461774 (+) 855 WP_046078548.1 formyltetrahydrofolate deformylase -
  UN84_RS06945 - 1462013..1462357 (+) 345 WP_046078549.1 hypothetical protein -
  UN84_RS06950 dinB 1462499..1463548 (-) 1050 WP_082090831.1 DNA polymerase IV -
  UN84_RS06955 lexA 1463739..1464401 (-) 663 WP_046078550.1 transcriptional repressor LexA -
  UN84_RS06960 - 1464646..1465290 (+) 645 WP_046078551.1 TetR/AcrR family transcriptional regulator -
  UN84_RS06965 pilC 1465359..1466585 (-) 1227 WP_046078552.1 type II secretion system F family protein Machinery gene
  UN84_RS06970 pilB 1466613..1468397 (-) 1785 WP_046078553.1 type IV-A pilus assembly ATPase PilB Machinery gene
  UN84_RS17515 - 1468704..1469093 (+) 390 WP_336885272.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  UN84_RS06980 - 1469266..1470720 (+) 1455 WP_046078554.1 O-antigen ligase family protein -
  UN84_RS06985 - 1470914..1471075 (-) 162 WP_237022376.1 hypothetical protein -
  UN84_RS17330 - 1471143..1471430 (-) 288 WP_202020712.1 transposase -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44809.60 Da        Isoelectric Point: 10.0077

>NTDB_id=237147 UN84_RS06965 WP_046078552.1 1465359..1466585(-) (pilC) [Halomonas sp. HG01]
MATASLNKAQKLKLYRWRWSGKGPQGRKVSGEIIARSRVEVEKELSGQNIIVKTIRRKSGNGMGKVKPRDIMLFARQMAT
MIRAGVPALQAFQVVAESMKKPAMRALVQELMNEVAAGASFSETLRRHPLYFDRLFCNLVEAGEQSGSLDRMLERVATYK
EKVESLKGRVKKALWYPAAVVTVGIGVTAILLIKVVPQFESLFHGFGAELPAMTRMTIQLSELAQQYWPWALGAVVAAIV
LIRAGIKRSEAFAYRMHALALRIPVIGDILDKSCVARYSRTLATTFGAGVPLVEALQTAAGASGNKVYERAVEQIRDDVA
TGQQLHFAMRLTEQFPPLAVQMVGIGEEAGALDAMLNRVADYYEEEVDNKVDALTSLLEPFIIVVLGVLVGGLVISMYLP
IFELGSVI

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=237147 UN84_RS06965 WP_046078552.1 1465359..1466585(-) (pilC) [Halomonas sp. HG01]
ATGGCAACCGCCAGCCTGAACAAGGCCCAGAAGCTCAAGCTGTATCGCTGGCGCTGGTCCGGCAAGGGGCCCCAGGGGCG
CAAGGTCAGCGGCGAGATCATCGCCAGGAGCCGCGTCGAGGTCGAGAAGGAACTCTCCGGCCAGAACATCATCGTCAAGA
CCATCCGCCGCAAGAGCGGCAACGGCATGGGCAAGGTGAAGCCCCGCGACATCATGCTGTTCGCTCGCCAGATGGCCACC
ATGATCCGCGCCGGCGTGCCGGCCCTGCAGGCCTTCCAGGTGGTCGCCGAGAGCATGAAGAAGCCGGCCATGCGCGCCCT
GGTGCAGGAGCTGATGAACGAGGTGGCCGCCGGCGCCAGCTTCTCCGAGACACTGAGGCGTCACCCGCTCTACTTCGACC
GGCTGTTCTGCAATCTGGTGGAGGCCGGTGAGCAGTCCGGCTCGCTGGATCGCATGCTCGAACGGGTGGCCACCTACAAG
GAAAAGGTGGAATCGCTCAAGGGTCGGGTAAAGAAGGCGCTGTGGTATCCGGCGGCGGTGGTCACGGTGGGCATCGGCGT
CACCGCGATCCTGCTGATCAAGGTGGTGCCGCAGTTCGAGAGCCTGTTCCACGGCTTCGGCGCCGAACTGCCGGCGATGA
CGCGCATGACCATCCAGCTCTCCGAACTGGCCCAGCAGTACTGGCCGTGGGCGCTGGGTGCGGTAGTCGCGGCCATCGTC
CTGATCCGCGCCGGCATCAAGCGCTCCGAGGCCTTCGCCTACCGCATGCATGCCCTGGCGCTGCGCATCCCGGTGATCGG
CGACATCCTCGACAAGTCCTGCGTGGCCCGCTACTCACGCACCCTGGCCACCACCTTCGGCGCCGGGGTGCCGCTGGTGG
AGGCGCTGCAGACCGCTGCCGGCGCCTCCGGCAACAAGGTCTACGAGCGCGCGGTGGAACAGATCCGCGACGACGTCGCC
ACCGGCCAGCAGCTGCACTTCGCCATGCGCCTGACCGAGCAGTTCCCGCCGCTGGCGGTGCAGATGGTCGGCATCGGCGA
GGAAGCCGGCGCCCTGGACGCCATGCTCAACCGGGTGGCGGACTACTACGAGGAAGAGGTCGACAACAAGGTCGACGCCC
TCACCTCGCTGCTCGAACCGTTCATCATCGTGGTGCTGGGCGTGCTGGTGGGCGGCCTGGTGATCTCGATGTACCTGCCG
ATCTTCGAGCTGGGGAGTGTGATTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(279-400)

Type II secretion system (T2SS), protein F (T2SSF)

(74-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

55.198

99.02

0.547
  pilC Acinetobacter baylyi ADP1

53.904

97.304

0.525
  pilC Acinetobacter baumannii D1279779

51.589

100

0.517
  pilC Legionella pneumophila strain ERS1305867

50.125

97.794

0.49
  pilC Vibrio cholerae strain A1552

44.963

99.755

0.449
  pilC Vibrio campbellii strain DS40M4

44.25

98.039

0.434
  pilG Neisseria gonorrhoeae MS11

41.96

97.549

0.409
  pilG Neisseria meningitidis 44/76-A

41.96

97.549

0.409
  pilC Thermus thermophilus HB27

36.186

100

0.363

Multiple sequence alignment