Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   UN84_RS06970 Genome accession   NZ_CP022134
Coordinates   1466613..1468397 (-) Length   594 a.a.
NCBI ID   WP_046078553.1    Uniprot ID   -
Organism   Halomonas sp. HG01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1461613..1473397
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UN84_RS06945 - 1462013..1462357 (+) 345 WP_046078549.1 hypothetical protein -
  UN84_RS06950 dinB 1462499..1463548 (-) 1050 WP_082090831.1 DNA polymerase IV -
  UN84_RS06955 lexA 1463739..1464401 (-) 663 WP_046078550.1 transcriptional repressor LexA -
  UN84_RS06960 - 1464646..1465290 (+) 645 WP_046078551.1 TetR/AcrR family transcriptional regulator -
  UN84_RS06965 pilC 1465359..1466585 (-) 1227 WP_046078552.1 type II secretion system F family protein Machinery gene
  UN84_RS06970 pilB 1466613..1468397 (-) 1785 WP_046078553.1 type IV-A pilus assembly ATPase PilB Machinery gene
  UN84_RS17515 - 1468704..1469093 (+) 390 WP_336885272.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  UN84_RS06980 - 1469266..1470720 (+) 1455 WP_046078554.1 O-antigen ligase family protein -
  UN84_RS06985 - 1470914..1471075 (-) 162 WP_237022376.1 hypothetical protein -
  UN84_RS17330 - 1471143..1471430 (-) 288 WP_202020712.1 transposase -
  UN84_RS06995 - 1471573..1472877 (-) 1305 WP_046078555.1 TRAP transporter large permease subunit -

Sequence

Protein


Download         Length: 594 a.a.        Molecular weight: 64699.59 Da        Isoelectric Point: 5.0021

>NTDB_id=237148 UN84_RS06970 WP_046078553.1 1466613..1468397(-) (pilB) [Halomonas sp. HG01]
MNDYRPPATEHQTSSQAPPSGAGRSHGMRGLAHRLVAEGLLAEAAAQRAEQEARDAETSLLEHVITTGLVQARSATLTAA
WEYGLPIIDLDALRLESLPDAADYPDRLLRKLGVLPLHRRGHRLTVAVPYPSTLAQLDELQFATGLTLEGILAPVDQLGP
VLEAYLARSESSMLDELSNVDDAVGELQFDEGVVDASDEQQAAATAGDDAPIVKFVNKILLDAIRRGASDIHFEPYETSY
RVRFRIDGILHDVAHPPFAMRSRIAARLKVMARLDISERRLPQDGAIKLKVSSSRSIDFRVNSLPTVYGEKLVLRILDPG
SAQMGIEQLGFTPEQRAMYEDVLDHPQGMILVTGPTGSGKTVTLYSGINILNEIERNICTAEDPVEIKVPGVNQVNVLPK
IGLDFASALRAFLRQDPDVVMVGEIRDLETAEIAVKASQTGHLVLSTVHTNSAAETLTRLANMGVAPFNIASSVSLIIAQ
RLARRLCPDCKQPADIPHEALLAQGFSEEEVQSATIYEPVGCQHCTQGFKGRVGIYEVVPISEAMSQLIMTHGNAMDFDT
QAREEGHPDLRRSGLFKVMQGMTSLAEVNRVTKD

Nucleotide


Download         Length: 1785 bp        

>NTDB_id=237148 UN84_RS06970 WP_046078553.1 1466613..1468397(-) (pilB) [Halomonas sp. HG01]
ATGAACGACTATCGCCCTCCCGCCACCGAACATCAGACCTCTTCTCAGGCGCCGCCTTCTGGCGCCGGCCGCTCCCACGG
CATGCGCGGACTGGCGCACCGGCTGGTCGCGGAGGGCCTGCTGGCCGAAGCGGCAGCACAGCGTGCCGAGCAGGAAGCCC
GGGATGCCGAGACCTCGCTGCTGGAGCACGTGATCACCACGGGCCTGGTCCAGGCCCGCTCCGCGACGCTCACGGCCGCC
TGGGAATATGGCCTGCCGATCATCGATCTGGATGCGCTGCGACTGGAAAGCCTGCCGGACGCCGCCGACTACCCCGACAG
GCTGCTGCGCAAACTCGGCGTGCTGCCGCTGCATCGCCGAGGGCACCGACTCACGGTGGCGGTGCCCTACCCCAGCACCC
TGGCCCAGCTCGACGAGCTGCAGTTCGCCACCGGCCTGACGCTGGAAGGCATCCTGGCCCCTGTCGACCAGCTCGGCCCG
GTGCTGGAGGCCTATCTGGCGCGCAGCGAATCCTCGATGCTCGACGAGCTGTCGAACGTCGATGACGCCGTGGGCGAGCT
GCAGTTCGACGAGGGCGTGGTCGATGCCAGCGACGAACAGCAGGCAGCGGCGACCGCCGGCGACGACGCGCCGATCGTCA
AGTTCGTCAACAAGATCCTGCTCGATGCCATCCGCCGTGGCGCCTCGGACATTCACTTCGAGCCCTACGAAACCAGTTAC
CGGGTGCGCTTCCGCATCGACGGCATCCTGCACGACGTGGCCCATCCGCCCTTCGCCATGCGCAGCCGCATCGCCGCGCG
CCTCAAGGTGATGGCCCGGCTGGACATCTCCGAGCGCCGCCTGCCCCAGGACGGCGCCATCAAGCTCAAGGTCTCGTCGA
GCCGCTCCATCGACTTCCGCGTCAACTCGCTGCCCACGGTCTACGGCGAGAAGCTGGTGCTGCGGATCCTCGACCCCGGC
TCGGCCCAGATGGGCATCGAACAGCTCGGCTTCACGCCCGAGCAGCGCGCCATGTACGAAGACGTGCTGGATCATCCCCA
GGGCATGATCCTGGTCACCGGCCCTACCGGCAGCGGCAAGACGGTCACGCTCTACAGCGGGATCAATATTCTCAACGAGA
TCGAACGCAACATCTGCACCGCCGAGGACCCGGTGGAAATCAAGGTCCCCGGCGTCAACCAGGTCAACGTGCTGCCCAAG
ATCGGGCTGGACTTCGCCAGCGCCCTGCGTGCCTTTCTGCGCCAGGACCCGGACGTGGTGATGGTCGGCGAGATCCGCGA
CCTGGAAACCGCCGAAATCGCGGTCAAGGCGTCCCAGACCGGGCACCTGGTGCTGTCCACCGTGCATACCAACTCCGCGG
CCGAGACCCTGACCCGGCTTGCCAACATGGGCGTGGCACCGTTCAATATCGCCAGCTCCGTGAGCCTGATCATCGCCCAG
CGCCTGGCCCGGCGGCTGTGCCCTGACTGCAAGCAGCCCGCCGACATCCCGCACGAGGCGCTGCTGGCTCAGGGCTTCAG
CGAGGAAGAAGTACAAAGCGCCACCATCTATGAACCGGTGGGATGCCAGCATTGCACCCAGGGCTTCAAGGGCCGGGTCG
GTATCTACGAGGTGGTGCCGATCAGTGAGGCCATGAGCCAGCTGATCATGACCCATGGCAACGCCATGGACTTCGACACC
CAGGCACGCGAGGAGGGCCACCCGGACCTCCGTCGCAGCGGCCTGTTCAAGGTCATGCAGGGCATGACCAGCCTGGCCGA
AGTCAACCGGGTCACCAAGGACTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(35-169)

Type II/IV secretion system protein (T2SSE)

(218-485)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

53.206

97.138

0.517
  pilB Acinetobacter baumannii D1279779

52.724

95.791

0.505
  pilB Acinetobacter baylyi ADP1

52.021

95.791

0.498
  pilB Vibrio campbellii strain DS40M4

47.276

95.791

0.453
  pilB Vibrio cholerae strain A1552

49.168

91.077

0.448
  pilB Vibrio parahaemolyticus RIMD 2210633

46.643

95.286

0.444
  pilF Neisseria gonorrhoeae MS11

44.991

95.791

0.431

Multiple sequence alignment