Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CEG15_RS02470 Genome accession   NZ_CP022099
Coordinates   485420..487108 (-) Length   562 a.a.
NCBI ID   WP_088720882.1    Uniprot ID   -
Organism   Vibrio anguillarum strain S3 4/9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 480420..492108
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEG15_RS02440 (CEG15_02440) rplS 481138..481491 (+) 354 WP_010319525.1 50S ribosomal protein L19 -
  CEG15_RS02445 (CEG15_02445) yacG 481572..481769 (-) 198 WP_010319526.1 DNA gyrase inhibitor YacG -
  CEG15_RS02450 (CEG15_02450) zapD 481859..482599 (-) 741 WP_013857689.1 cell division protein ZapD -
  CEG15_RS02455 (CEG15_02455) coaE 482632..483240 (-) 609 WP_064624393.1 dephospho-CoA kinase -
  CEG15_RS02460 (CEG15_02460) pilD 483231..484112 (-) 882 WP_088720881.1 prepilin peptidase Machinery gene
  CEG15_RS02465 (CEG15_02465) pilC 484151..485377 (-) 1227 WP_088728613.1 type II secretion system F family protein Machinery gene
  CEG15_RS02470 (CEG15_02470) pilB 485420..487108 (-) 1689 WP_088720882.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CEG15_RS02475 (CEG15_02475) pilA 487118..487543 (-) 426 WP_010319532.1 pilin Machinery gene
  CEG15_RS02480 (CEG15_02480) nadC 487802..488689 (-) 888 WP_017043701.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CEG15_RS02485 (CEG15_02485) ampD 488814..489365 (+) 552 WP_232471667.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CEG15_RS02490 (CEG15_02490) fldB 489387..489905 (-) 519 WP_088720885.1 flavodoxin FldB -
  CEG15_RS02495 (CEG15_02495) xerD 490058..490966 (+) 909 WP_013857679.1 site-specific tyrosine recombinase XerD -
  CEG15_RS02500 (CEG15_02500) - 490980..491753 (+) 774 WP_013857678.1 thioredoxin fold domain-containing protein -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62270.40 Da        Isoelectric Point: 6.2447

>NTDB_id=236721 CEG15_RS02470 WP_088720882.1 485420..487108(-) (pilB) [Vibrio anguillarum strain S3 4/9]
MLTNLPAILRQAQILSPTQEHAVIEHIKASGASVPEALLALDFFHPHDLTEQLSTLFGLPDTDLTRYDYPLLCQQLGLRD
LITRYQALPIANNGQTLTIAVSDPTHLQVEDDFRFATGLQVELVLADYKALQAAIRRLYGRTIQGSSSSGKEISQDELAN
LVKLSDDEIQSIEDLSQDDAPVSRFINQILLDAVRKGASDIHFEPYEALYRVRLRCDGILVETQRPSSNLSRRLAARLKI
LSKLDIAERRLPQDGRIKLRLNQDTAIDMRVSSLPTLWGEKIVLRLLDSSAANLDIDKLGYNDQQKQLYLAALKKPQGMI
LMTGPTGSGKTVSLYTGLRILNTDEVNISTAEDPVEINLSGINQVQVQPKIGFGFAQALRSFLRQDPDIVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLSNMGIEAFNLASSLSLIIAQRLARRLCRHCKIAVVPSMQLQQHFQLLATE
TIYEANPHGCNECTGGYSGRVGIYEVMAFNPQLAEAIMQKASIHQIEQIAKANGMQTLQESGIEKLKNGVTSFKELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=236721 CEG15_RS02470 WP_088720882.1 485420..487108(-) (pilB) [Vibrio anguillarum strain S3 4/9]
ATGCTAACCAATCTCCCTGCGATTCTGCGTCAGGCTCAGATATTGAGCCCGACGCAAGAACACGCAGTTATTGAACATAT
CAAAGCGTCTGGTGCGAGTGTGCCAGAAGCTTTGCTCGCTTTGGATTTTTTCCATCCTCATGATCTGACTGAGCAATTAA
GTACCCTTTTTGGCCTACCAGACACCGACTTAACCCGCTACGACTACCCACTGCTCTGCCAACAGCTTGGTTTGCGTGAT
CTCATCACCCGTTACCAAGCACTGCCGATTGCCAATAATGGCCAAACCCTGACCATCGCAGTTTCCGACCCGACCCATTT
ACAAGTGGAAGATGATTTTCGCTTTGCGACAGGCTTACAAGTCGAACTGGTTTTAGCCGACTACAAAGCGCTGCAAGCAG
CCATCAGGCGCTTGTATGGCCGAACAATTCAAGGATCCTCTTCCTCAGGGAAAGAGATCAGCCAAGATGAACTGGCGAAC
TTAGTTAAGCTGTCTGATGATGAGATTCAATCGATTGAAGACCTCAGCCAAGATGACGCCCCAGTCAGTCGCTTTATTAA
CCAAATCCTGCTTGATGCGGTGCGAAAAGGCGCATCAGACATCCATTTCGAACCGTACGAAGCGCTTTACCGAGTACGAT
TGCGCTGTGATGGTATTTTGGTGGAAACGCAGCGTCCCTCAAGCAACTTAAGTCGCCGGCTTGCCGCGCGGCTTAAAATT
TTATCCAAACTCGATATTGCCGAGCGGCGCTTACCACAAGATGGACGAATTAAACTGCGGTTGAATCAAGACACCGCCAT
CGATATGCGCGTTTCCAGCCTCCCCACTTTATGGGGTGAAAAAATCGTACTGCGCCTACTTGATAGCAGCGCTGCTAACT
TAGATATCGACAAATTGGGTTATAACGACCAGCAAAAACAACTCTACCTAGCTGCGCTGAAAAAACCACAAGGGATGATT
TTAATGACGGGCCCGACGGGCAGCGGAAAAACCGTCTCGCTCTATACGGGGCTACGCATTTTAAATACTGATGAAGTGAA
CATTTCTACCGCTGAAGATCCGGTTGAAATTAACTTATCCGGTATCAACCAAGTTCAAGTGCAGCCAAAAATTGGCTTTG
GCTTTGCGCAAGCATTACGTTCTTTCTTACGGCAAGATCCCGACATTGTGATGGTTGGCGAAATCCGCGACTTAGAAACT
GCCGAAATCGCCGTGAAAGCAGCACAAACCGGGCATTTAGTCCTTTCTACCTTGCATACTAACTCCGCCGCCGAAACGGT
GATTCGCTTGAGTAATATGGGAATTGAAGCGTTTAACTTAGCCTCTTCACTCAGTTTGATTATTGCCCAGCGTTTAGCTC
GTCGCCTTTGTCGGCACTGCAAAATTGCCGTCGTGCCCTCAATGCAGTTGCAGCAACATTTTCAGCTGCTAGCGACAGAA
ACCATTTATGAAGCGAACCCGCATGGTTGCAACGAATGCACAGGGGGCTATTCAGGTCGAGTCGGCATTTATGAAGTGAT
GGCGTTTAACCCACAGCTGGCCGAAGCGATTATGCAAAAAGCAAGCATTCATCAAATAGAACAGATCGCGAAAGCCAACG
GAATGCAGACACTACAAGAATCAGGCATTGAAAAGCTCAAAAACGGCGTCACCAGCTTCAAAGAGCTGCAACGCGTGCTC
TATTTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

83.274

100

0.833
  pilB Vibrio campbellii strain DS40M4

74.555

100

0.746
  pilB Vibrio parahaemolyticus RIMD 2210633

73.132

100

0.731
  pilB Acinetobacter baumannii D1279779

51.923

92.527

0.48
  pilB Legionella pneumophila strain ERS1305867

51.758

91.103

0.472
  pilB Acinetobacter baylyi ADP1

50.38

93.594

0.472
  pilF Neisseria gonorrhoeae MS11

44.248

100

0.445
  pilB/pilB1 Synechocystis sp. PCC 6803

36.149

100

0.381
  pilF Thermus thermophilus HB27

37.213

100

0.375

Multiple sequence alignment