Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   CEJ98_RS02600 Genome accession   NZ_CP022005
Coordinates   551003..552163 (-) Length   386 a.a.
NCBI ID   WP_036052882.1    Uniprot ID   -
Organism   Burkholderia gladioli pv. gladioli strain KACC 11889     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 546003..557163
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEJ98_RS02570 (CEJ98_02570) - 546561..547001 (+) 441 WP_036048346.1 NUDIX domain-containing protein -
  CEJ98_RS02575 (CEJ98_02575) - 547118..547318 (-) 201 WP_013696563.1 DNA gyrase inhibitor YacG -
  CEJ98_RS02580 (CEJ98_02580) zapD 547323..548078 (-) 756 WP_013696564.1 cell division protein ZapD -
  CEJ98_RS02585 (CEJ98_02585) coaE 548234..548842 (-) 609 WP_036048343.1 dephospho-CoA kinase -
  CEJ98_RS02590 (CEJ98_02590) - 548847..549737 (-) 891 WP_036048341.1 prepilin peptidase -
  CEJ98_RS02595 (CEJ98_02595) - 549798..551006 (-) 1209 WP_036048339.1 type II secretion system F family protein -
  CEJ98_RS02600 (CEJ98_02600) pilF 551003..552163 (-) 1161 WP_036052882.1 GspE/PulE family protein Machinery gene
  CEJ98_RS02605 (CEJ98_02605) - 552400..553695 (-) 1296 WP_025096841.1 HlyC/CorC family transporter -
  CEJ98_RS02615 (CEJ98_02615) - 553943..554935 (-) 993 WP_036038944.1 polyprenyl synthetase family protein -
  CEJ98_RS02620 (CEJ98_02620) rplU 555313..555624 (+) 312 WP_004194344.1 50S ribosomal protein L21 -
  CEJ98_RS02625 (CEJ98_02625) rpmA 555652..555915 (+) 264 WP_013696571.1 50S ribosomal protein L27 -

Sequence

Protein


Download         Length: 386 a.a.        Molecular weight: 41115.37 Da        Isoelectric Point: 8.3251

>NTDB_id=235808 CEJ98_RS02600 WP_036052882.1 551003..552163(-) (pilF) [Burkholderia gladioli pv. gladioli strain KACC 11889]
MVALVNEILKAATARDASDIHIEPAEQGWRVRLRIDGALHPFAAPPAHLRDGVVTRLKVLSRLDIAERRVPQDGRLRIAL
APGLTGDYRVSSLPTLHGEKLVLRRLDTLPANLSLSSLGFDARQAGALEAAVAAPHGLVLVTGPTGSGKTVTLYCCLQGL
NLASRNLCTIEDPAELQLDGLNQVNVRDKAGLSFAVALRAFLRQDPDVIMVGEIRDAETADVALKAAQTGHLVLSTLHTN
DAPGAIARLLDIGVAPYHIAAALRIVTAQRLVRRLCVTCRRPSRESIPTLLAAGLDPTRLEDGWRPFVAAGCEACHGIGY
RGRIGVHQVMAVSDTLRERIVARAGSLALTRQAQADGMRSLREAALARVHDGSTSLDEACAVAEAG

Nucleotide


Download         Length: 1161 bp        

>NTDB_id=235808 CEJ98_RS02600 WP_036052882.1 551003..552163(-) (pilF) [Burkholderia gladioli pv. gladioli strain KACC 11889]
GTGGTCGCGCTGGTCAACGAGATCCTCAAGGCCGCCACCGCGCGCGACGCCTCCGACATCCATATCGAACCGGCCGAGCA
AGGCTGGCGCGTGCGATTGCGCATCGACGGCGCGCTCCATCCGTTCGCCGCGCCGCCCGCGCACCTGCGCGACGGCGTGG
TCACGCGCCTGAAGGTGCTGTCGCGCCTGGACATCGCCGAGCGGCGCGTGCCGCAGGATGGCCGCCTGCGCATCGCCCTC
GCGCCGGGCCTGACGGGCGACTACCGCGTCAGCTCGCTGCCCACGCTGCACGGCGAGAAGCTGGTGCTGCGCCGCCTCGA
CACGCTGCCGGCCAACCTGTCGCTATCCTCGCTCGGCTTCGATGCGCGGCAGGCCGGCGCGCTCGAAGCGGCCGTCGCCG
CGCCGCACGGGCTGGTGCTGGTGACAGGGCCGACCGGCAGCGGCAAGACCGTCACGCTCTATTGCTGTTTGCAGGGGCTC
AATCTAGCCTCGCGCAATCTCTGCACCATCGAGGATCCGGCCGAGCTGCAACTCGATGGGCTGAACCAGGTCAATGTGCG
CGACAAGGCCGGCCTGAGCTTCGCGGTCGCGCTGCGCGCCTTCCTGCGCCAGGATCCGGACGTGATCATGGTCGGCGAGA
TCCGTGACGCCGAAACCGCCGACGTGGCGCTGAAGGCCGCGCAGACCGGCCACCTGGTGTTGTCGACGCTGCATACCAAC
GATGCGCCGGGCGCGATCGCGCGCCTGCTCGACATCGGCGTGGCGCCCTATCACATCGCCGCGGCGCTGAGGATCGTCAC
CGCCCAGCGCCTGGTGCGCCGGCTCTGCGTCACATGCCGCCGGCCTTCGCGCGAGTCGATCCCGACCCTGCTCGCGGCCG
GCCTCGATCCGACCCGGCTCGAAGACGGCTGGCGCCCCTTCGTCGCGGCCGGTTGCGAGGCCTGCCATGGCATCGGCTAT
CGCGGCCGGATCGGCGTGCATCAGGTGATGGCGGTTTCCGACACGCTGCGCGAGCGGATCGTCGCCCGCGCGGGCAGCCT
CGCGCTCACGCGCCAGGCGCAGGCCGACGGCATGCGCTCGCTGCGCGAGGCCGCCCTGGCTCGCGTGCACGACGGCAGCA
CCAGCCTCGACGAGGCCTGCGCGGTCGCCGAGGCCGGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

48.947

98.446

0.482
  pilB Vibrio cholerae strain A1552

49.067

97.15

0.477
  pilB Acinetobacter baumannii D1279779

47.52

99.223

0.472
  pilB Acinetobacter baylyi ADP1

48.021

98.187

0.472
  pilB Vibrio campbellii strain DS40M4

47.594

96.891

0.461
  pilB Legionella pneumophila strain ERS1305867

46.094

99.482

0.459
  pilB Vibrio parahaemolyticus RIMD 2210633

47.326

96.891

0.459
  pilF Thermus thermophilus HB27

44.974

97.927

0.44
  pilB/pilB1 Synechocystis sp. PCC 6803

42.785

100

0.438
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.125

99.223

0.438
  pilB Glaesserella parasuis strain SC1401

43.008

98.187

0.422
  pilB Haemophilus influenzae 86-028NP

41.799

97.927

0.409
  pilB Haemophilus influenzae Rd KW20

46.061

85.492

0.394
  ctsE Campylobacter jejuni subsp. jejuni 81-176

38.667

97.15

0.376

Multiple sequence alignment