Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   BB165_RS01005 Genome accession   NZ_CP021870
Coordinates   167683..168654 (+) Length   323 a.a.
NCBI ID   WP_000250808.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain SG-M4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 162683..173654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BB165_RS00990 (BB165_00990) rpoC 163381..167031 (+) 3651 WP_000228729.1 DNA-directed RNA polymerase subunit beta' -
  BB165_RS00995 (BB165_00995) - 167145..167510 (+) 366 WP_000285373.1 DUF1033 family protein -
  BB165_RS01005 (BB165_01005) comYA 167683..168654 (+) 972 WP_000250808.1 competence type IV pilus ATPase ComGA Machinery gene
  BB165_RS01010 (BB165_01010) comYB 168548..169591 (+) 1044 WP_223295592.1 competence type IV pilus assembly protein ComGB Machinery gene
  BB165_RS01015 (BB165_01015) comGF 169588..170043 (+) 456 WP_000793378.1 competence type IV pilus minor pilin ComGF -
  BB165_RS01020 (BB165_01020) comGG 170021..170392 (+) 372 WP_000601104.1 competence type IV pilus minor pilin ComGG -
  BB165_RS01025 (BB165_01025) comYH 170507..171481 (+) 975 WP_001008570.1 class I SAM-dependent methyltransferase Machinery gene
  BB165_RS01030 (BB165_01030) - 171513..172706 (+) 1194 WP_000047535.1 acetate kinase -
  BB165_RS01035 (BB165_01035) - 172857..173063 (+) 207 WP_000798242.1 helix-turn-helix transcriptional regulator -
  BB165_RS01040 (BB165_01040) - 173122..173259 (+) 138 WP_001867090.1 hypothetical protein -

Sequence

Protein


Download         Length: 323 a.a.        Molecular weight: 36779.23 Da        Isoelectric Point: 6.8469

>NTDB_id=234261 BB165_RS01005 WP_000250808.1 167683..168654(+) (comYA) [Streptococcus agalactiae strain SG-M4]
MVQSLAKQVIHQAVEVNAQDIYIIPKGDCYELYMRIDDERRFIDVFEFNRMASLISHFKFVAGMNVGEKRRSQLGSCDYE
LSEGRLVSLRLSSVGDYRGQESLVIRILYSGHQDLKYWFDNIKQMKEVLGTRGLYLFSGPVGSGKTTLMYQLASEVFKNK
QIITIEDPVEIKNDKMLQLQLNEDIGMTYDALIKLSLRHRPDILIIGEIRDQATARAVIRASLTGVMVFSTIHAKSISGV
YDRLIELGVNYQELENSLKLIAYQRLIGGGSLIDFETGNFKKHSSDKWNRQVDILAEEGHISKKQAQVEKIIPQETTESS
PTF

Nucleotide


Download         Length: 972 bp        

>NTDB_id=234261 BB165_RS01005 WP_000250808.1 167683..168654(+) (comYA) [Streptococcus agalactiae strain SG-M4]
ATGGTTCAATCATTAGCAAAGCAAGTCATTCATCAGGCAGTAGAAGTAAATGCTCAAGATATTTATATCATTCCCAAAGG
TGATTGTTATGAACTCTATATGCGTATTGATGATGAAAGGCGGTTTATTGATGTTTTTGAGTTTAATAGGATGGCTAGTC
TTATTAGTCACTTTAAATTTGTGGCAGGCATGAACGTTGGAGAAAAAAGACGAAGTCAATTAGGTTCTTGTGACTATGAA
CTGTCAGAGGGAAGACTGGTTTCATTACGACTATCGAGTGTGGGAGATTATCGTGGTCAAGAATCTTTAGTTATTCGTAT
TTTGTATTCAGGTCATCAGGACTTAAAATATTGGTTTGATAATATAAAGCAAATGAAGGAAGTACTGGGTACAAGAGGGC
TATATCTTTTTTCCGGCCCTGTGGGGAGTGGTAAAACAACTCTCATGTATCAATTAGCTTCAGAAGTATTTAAAAATAAG
CAAATTATCACGATTGAAGATCCGGTAGAAATCAAGAATGACAAGATGTTACAACTCCAATTGAATGAGGATATTGGAAT
GACTTATGATGCTTTAATCAAACTGTCTTTACGGCATCGTCCAGATATTTTAATTATCGGAGAGATTAGAGATCAAGCGA
CGGCCCGTGCTGTTATTCGTGCAAGTTTAACGGGAGTGATGGTTTTTTCTACTATTCATGCTAAAAGTATTTCCGGAGTC
TATGATAGGCTTATAGAATTAGGGGTTAACTATCAAGAGTTAGAAAATAGTCTAAAATTAATAGCATATCAACGTTTAAT
TGGAGGAGGAAGCCTAATTGACTTTGAGACAGGTAACTTTAAAAAACACTCATCAGACAAGTGGAATAGACAAGTGGATA
TCTTGGCTGAAGAAGGACATATCAGTAAGAAACAGGCACAAGTCGAAAAAATTATCCCTCAAGAAACAACGGAAAGTAGT
CCAACTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

68.489

96.285

0.659
  comYA Streptococcus mutans UA140

68.489

96.285

0.659
  comYA Streptococcus gordonii str. Challis substr. CH1

63.323

98.762

0.625
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

62.701

96.285

0.604
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

61.736

96.285

0.594
  comGA/cglA/cilD Streptococcus pneumoniae D39

61.736

96.285

0.594
  comGA/cglA/cilD Streptococcus pneumoniae R6

61.736

96.285

0.594
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

61.736

96.285

0.594
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.613

95.975

0.591
  comGA Lactococcus lactis subsp. cremoris KW2

50.621

99.69

0.505

Multiple sequence alignment