Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   BB165_RS01010 Genome accession   NZ_CP021870
Coordinates   168548..169591 (+) Length   347 a.a.
NCBI ID   WP_223295592.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain SG-M4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 163548..174591
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BB165_RS00995 (BB165_00995) - 167145..167510 (+) 366 WP_000285373.1 DUF1033 family protein -
  BB165_RS01005 (BB165_01005) comYA 167683..168654 (+) 972 WP_000250808.1 competence type IV pilus ATPase ComGA Machinery gene
  BB165_RS01010 (BB165_01010) comYB 168548..169591 (+) 1044 WP_223295592.1 competence type IV pilus assembly protein ComGB Machinery gene
  BB165_RS01015 (BB165_01015) comGF 169588..170043 (+) 456 WP_000793378.1 competence type IV pilus minor pilin ComGF -
  BB165_RS01020 (BB165_01020) comGG 170021..170392 (+) 372 WP_000601104.1 competence type IV pilus minor pilin ComGG -
  BB165_RS01025 (BB165_01025) comYH 170507..171481 (+) 975 WP_001008570.1 class I SAM-dependent methyltransferase Machinery gene
  BB165_RS01030 (BB165_01030) - 171513..172706 (+) 1194 WP_000047535.1 acetate kinase -
  BB165_RS01035 (BB165_01035) - 172857..173063 (+) 207 WP_000798242.1 helix-turn-helix transcriptional regulator -
  BB165_RS01040 (BB165_01040) - 173122..173259 (+) 138 WP_001867090.1 hypothetical protein -
  BB165_RS01045 (BB165_01045) - 173300..173755 (+) 456 WP_000905674.1 hypothetical protein -
  BB165_RS01050 (BB165_01050) - 173824..174489 (+) 666 WP_000008111.1 type II CAAX endopeptidase family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 40005.70 Da        Isoelectric Point: 9.9365

>NTDB_id=234262 BB165_RS01010 WP_223295592.1 168548..169591(+) (comYB) [Streptococcus agalactiae strain SG-M4]
MDKWISWLKKDISVRNRHKSKKLSLKKQRKVVQLFNNLFASGFSLTDMVTFLKRSKLLSDYYTDRMNKALLEGKDLSKML
GELGFSDTVITQVALADLHGNISRSLLKIESYLANLLLVRKKVIEVATYPLILLTFLVLIMIGLRHYLMPQLGENNFATS
LITNVPNIFLLLLAVVLIFSLIFYIVQKRLSRIKVACFLTTIPLVGSYVKLYLTAYYAREWGNLLSQGVELDQIVKVMQN
QKSKLFREIGYDMEEGFLSGKAFHQKVLDYPFFLTELSLMIEYGQVKAKLGTELDIYADEKWEDFFTKLARATQLIQPVI
FIFVALIIVMIYAAMLLPMYQNMEILS

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=234262 BB165_RS01010 WP_223295592.1 168548..169591(+) (comYB) [Streptococcus agalactiae strain SG-M4]
ATAGACAAGTGGATATCTTGGCTGAAGAAGGACATATCAGTAAGAAACAGGCACAAGTCGAAAAAATTATCCCTCAAGAA
ACAACGGAAAGTAGTCCAACTTTTTAATAATCTTTTTGCTAGTGGTTTTTCTTTAACTGATATGGTCACCTTCTTAAAGA
GGAGTAAGTTATTGTCTGATTATTATACAGATAGGATGAATAAGGCATTATTAGAGGGAAAAGATTTATCAAAAATGTTA
GGAGAGTTAGGTTTTTCTGACACTGTTATCACACAGGTTGCATTAGCTGATTTGCATGGTAACATTTCAAGGAGCCTACT
AAAGATTGAGTCTTATTTAGCTAATCTTTTGTTAGTTAGAAAAAAAGTAATAGAAGTAGCTACTTACCCATTGATATTAT
TGACTTTTCTGGTGCTAATTATGATTGGTCTTAGGCATTATTTAATGCCCCAATTAGGAGAAAATAATTTTGCAACTAGC
CTGATTACAAATGTGCCGAATATTTTCTTATTACTTTTAGCAGTTGTACTTATTTTTAGTTTAATATTTTATATTGTTCA
AAAGCGATTGTCGCGTATTAAAGTAGCTTGTTTTTTAACAACAATTCCCTTAGTTGGATCATATGTTAAGCTTTATTTAA
CTGCTTACTATGCCCGTGAGTGGGGAAATTTATTAAGTCAAGGTGTTGAATTGGACCAAATTGTTAAAGTAATGCAAAAT
CAGAAATCCAAACTTTTTAGGGAAATAGGATATGACATGGAAGAAGGTTTTCTATCAGGTAAAGCATTTCACCAAAAAGT
ATTAGACTATCCGTTTTTCTTAACTGAGCTTAGTTTAATGATTGAATATGGCCAAGTTAAGGCGAAATTAGGAACAGAGT
TAGATATATATGCTGATGAGAAGTGGGAGGATTTTTTTACAAAATTAGCTAGAGCGACCCAGTTAATCCAACCTGTTATT
TTTATTTTTGTAGCTCTTATCATTGTTATGATTTATGCAGCAATGCTGTTACCAATGTATCAAAATATGGAGATATTATC
ATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(218-338)

Type II secretion system (T2SS), protein F (T2SSF)

(33-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

61.877

98.271

0.608
  comYB Streptococcus mutans UA159

61.584

98.271

0.605
  comYB Streptococcus gordonii str. Challis substr. CH1

56.305

98.271

0.553
  comGB/cglB Streptococcus mitis NCTC 12261

54.925

96.542

0.53
  comGB/cglB Streptococcus mitis SK321

54.328

96.542

0.524
  comGB/cglB Streptococcus pneumoniae Rx1

53.433

96.542

0.516
  comGB/cglB Streptococcus pneumoniae D39

53.433

96.542

0.516
  comGB/cglB Streptococcus pneumoniae R6

53.433

96.542

0.516
  comGB/cglB Streptococcus pneumoniae TIGR4

53.433

96.542

0.516
  comGB Lactococcus lactis subsp. cremoris KW2

49.852

97.118

0.484

Multiple sequence alignment