Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   EF_RS02445 Genome accession   NC_004668
Coordinates   462000..462458 (+) Length   152 a.a.
NCBI ID   WP_002365911.1    Uniprot ID   Q8VT46
Organism   Enterococcus faecalis V583     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 455113..505559 462000..462458 within 0


Gene organization within MGE regions


Location: 455113..505559
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EF_RS02395 (EF0489) - 455366..456244 (+) 879 WP_002377931.1 hypothetical protein -
  EF_RS02400 (EF0490) - 456264..457079 (+) 816 WP_010773698.1 LPXTG cell wall anchor domain-containing protein -
  EF_RS02405 (EF0491) - 457105..458376 (+) 1272 WP_011109451.1 CHAP domain-containing protein -
  EF_RS02410 (EF0492) - 458379..458996 (+) 618 WP_002377936.1 hypothetical protein -
  EF_RS02415 (EF0493) - 458980..459396 (+) 417 WP_002401419.1 thioredoxin domain-containing protein -
  EF_RS02420 (EF0494) - 459396..459710 (+) 315 WP_002360796.1 hypothetical protein -
  EF_RS02425 (EF0495) - 459976..460737 (+) 762 WP_010773700.1 conjugal transfer protein -
  EF_RS02430 (EF0496) - 460742..461002 (+) 261 WP_010706916.1 hypothetical protein -
  EF_RS02435 (EF0497) - 461002..461391 (+) 390 WP_002360791.1 TcpE family conjugal transfer membrane protein -
  EF_RS02440 (EF0498) - 461423..461905 (+) 483 WP_002377938.1 hypothetical protein -
  EF_RS02445 (EF0499) ssb 462000..462458 (+) 459 WP_002365911.1 single-stranded DNA-binding protein Machinery gene
  EF_RS02450 (EF0500) - 462563..465055 (+) 2493 WP_002377939.1 ATP-binding protein -
  EF_RS02455 (EF0501) - 465012..465410 (+) 399 WP_002370287.1 lipocalin-like domain-containing protein -
  EF_RS02460 (EF0502) - 465423..467768 (+) 2346 WP_002377940.1 CD3337/EF1877 family mobilome membrane protein -
  EF_RS02465 (EF0503) - 467755..470013 (+) 2259 WP_010774165.1 type IV secretory system conjugative DNA transfer family protein -
  EF_RS02470 (EF0505) - 470536..471321 (+) 786 WP_002360778.1 replication-relaxation family protein -
  EF_RS02475 (EF0506) - 471327..471815 (+) 489 WP_010710133.1 hypothetical protein -
  EF_RS02480 (EF0507) - 472407..472673 (+) 267 WP_002377947.1 hypothetical protein -
  EF_RS02485 (EF0508) - 472706..473206 (+) 501 WP_002360775.1 DnaJ domain-containing protein -
  EF_RS02490 (EF0509) - 473219..473467 (+) 249 WP_002360773.1 DUF3850 domain-containing protein -
  EF_RS02495 (EF0510) - 473543..473959 (+) 417 WP_010710134.1 single-stranded DNA-binding protein -
  EF_RS02500 (EF0511) - 473983..474567 (+) 585 WP_002377949.1 thermonuclease family protein -
  EF_RS02505 (EF0512) - 474678..474944 (+) 267 WP_002369771.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
  EF_RS02510 (EF0513) - 474937..475212 (+) 276 WP_002360769.1 type II toxin-antitoxin system YafQ family toxin -
  EF_RS02515 (EF0514) - 475403..476047 (-) 645 WP_002377952.1 IS6 family transposase -
  EF_RS02520 - 476180..477062 (+) 883 Protein_479 IS256 family transposase -
  EF_RS02525 (EF0516) - 477367..478404 (-) 1038 WP_002355369.1 PTS sugar transporter subunit IIC -
  EF_RS02530 (EF0517) - 478430..479368 (-) 939 WP_002370935.1 2-dehydropantoate 2-reductase -
  EF_RS02535 (EF0518) - 479496..479765 (-) 270 Protein_482 LPXTG cell wall anchor domain-containing protein -
  EF_RS02540 (EF0519) - 479855..480040 (+) 186 WP_002358660.1 hypothetical protein -
  EF_RS02545 (EF0520) - 480072..480680 (+) 609 Protein_484 IS6 family transposase -
  EF_RS02550 (EF0521) bsh 481349..482323 (-) 975 WP_002355428.1 choloylglycine hydrolase -
  EF_RS02555 (EF0522) - 482547..483191 (+) 645 WP_002377952.1 IS6 family transposase -
  EF_RS02560 (EF0523) - 483364..483678 (+) 315 WP_002403043.1 hypothetical protein -
  EF_RS02565 (EF0524) cylR2 484016..484216 (-) 201 WP_002370931.1 cytolysin regulator CylR2 -
  EF_RS02570 (EF0525) cylL-L 484620..484826 (+) 207 WP_002358485.1 lanthipeptide cytolysin subunit CylL-L -
  EF_RS02575 (EF0526) cylL-S 484860..485051 (+) 192 WP_002358181.1 lanthipeptide cytolysin subunit CylL-S -
  EF_RS02580 (EF0527) - 485112..488093 (+) 2982 WP_002370929.1 type 2 lanthipeptide synthetase LanM family protein -
  EF_RS02585 (EF0528) - 488105..488917 (+) 813 WP_010774166.1 cysteine peptidase family C39 domain-containing protein -
  EF_RS02590 (EF0529) - 489003..490175 (+) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  EF_RS02595 (EF0530) - 490245..491147 (+) 903 Protein_494 YSIRK-targeted surface antigen transcriptional regulator -
  EF_RS02600 (EF0531) - 491277..491693 (+) 417 WP_002358522.1 hypothetical protein -
  EF_RS16525 - 491829..491930 (-) 102 Protein_496 IS30 family transposase -
  EF_RS02605 (EF0533) - 491918..492655 (+) 738 WP_002401428.1 hypothetical protein -
  EF_RS02610 (EF0534) - 492701..493321 (-) 621 WP_033591050.1 recombinase family protein -
  EF_RS02615 (EF0536) - 493505..494124 (+) 620 Protein_499 recombinase family protein -
  EF_RS02620 - 494233..494415 (+) 183 WP_002358529.1 hypothetical protein -
  EF_RS02625 (EF0539) - 494871..495689 (-) 819 WP_002358531.1 MurR/RpiR family transcriptional regulator -
  EF_RS02630 (EF0540) - 495845..496552 (+) 708 WP_002358533.1 N-acetylmannosamine-6-phosphate 2-epimerase -
  EF_RS02635 (EF0541) - 496545..498047 (+) 1503 WP_002358534.1 PTS transporter subunit EIIC -
  EF_RS02640 - 498058..498540 (+) 483 WP_002358535.1 PTS sugar transporter subunit IIA -
  EF_RS02645 (EF0542) - 499173..499937 (-) 765 WP_002358536.1 YibE/F family protein -
  EF_RS02650 (EF0543) - 499934..500989 (-) 1056 WP_002401430.1 YibE/F family protein -
  EF_RS02655 (EF0544) rpmF 501328..501501 (+) 174 WP_002358539.1 50S ribosomal protein L32 -
  EF_RS02660 (EF0545) - 501523..501900 (+) 378 WP_002382844.1 hypothetical protein -
  EF_RS02665 (EF0546) - 501952..502356 (+) 405 WP_002358541.1 NusG domain II-containing protein -
  EF_RS02670 (EF0547) - 502550..503620 (+) 1071 WP_010710139.1 ammonium transporter -
  EF_RS02675 (EF0548) - 503723..504856 (+) 1134 WP_002377959.1 NAD(P)/FAD-dependent oxidoreductase -
  EF_RS16995 - 505057..505388 (-) 332 Protein_512 PTS glucose transporter subunit IIA -

Sequence


Protein


Download         Length: 152 a.a.        Molecular weight: 17179.08 Da        Isoelectric Point: 4.6511

>NTDB_id=22869 EF_RS02445 WP_002365911.1 462000..462458(+) (ssb) [Enterococcus faecalis V583]
MINNVTLVGRLTKDPDLRYTQSGTAVGQFTLAINRNFTNANNEREADFINCVIWRKAAESLANYATKGTLIGLTGRIQTR
NYENQQGQRIYVTEVVTESFQLLESREVNEQRKEQATGKATFDKQSMDKPDPLDPFSPENSIVDISDNDLPF

Nucleotide


Download         Length: 459 bp        

>NTDB_id=22869 EF_RS02445 WP_002365911.1 462000..462458(+) (ssb) [Enterococcus faecalis V583]
TTGATTAATAACGTTACATTAGTTGGACGATTAACCAAAGACCCAGATTTAAGGTATACGCAAAGTGGAACAGCCGTAGG
TCAATTTACGTTGGCCATTAATCGCAACTTTACCAATGCTAACAATGAAAGGGAAGCAGATTTTATCAACTGTGTTATTT
GGCGGAAAGCTGCAGAGTCATTAGCAAATTATGCAACAAAAGGGACTCTGATCGGTTTAACTGGTCGCATTCAAACAAGA
AACTATGAGAATCAACAAGGCCAGCGTATTTATGTAACTGAGGTTGTCACAGAAAGCTTCCAACTATTAGAATCAAGAGA
AGTAAACGAGCAACGAAAAGAACAGGCTACAGGTAAAGCTACGTTTGATAAACAGTCAATGGATAAACCTGATCCTCTGG
ATCCATTTTCGCCAGAAAATAGCATAGTGGATATTTCTGATAATGACCTGCCGTTTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB Q8VT46

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

56.471

100

0.632

  ssbA Bacillus subtilis subsp. subtilis str. 168

47.283

100

0.572

  ssbB Bacillus subtilis subsp. subtilis str. 168

56.604

69.737

0.395

  ssbB/cilA Streptococcus mitis NCTC 12261

50

76.316

0.382

  ssbB Streptococcus sobrinus strain NIDR 6715-7

53.774

69.737

0.375

  ssbB/cilA Streptococcus pneumoniae TIGR4

49.138

76.316

0.375

  ssbB/cilA Streptococcus pneumoniae Rx1

48.276

76.316

0.368

  ssbB/cilA Streptococcus pneumoniae D39

48.276

76.316

0.368

  ssbB/cilA Streptococcus pneumoniae R6

48.276

76.316

0.368

  ssbB/cilA Streptococcus mitis SK321

48.276

76.316

0.368


Multiple sequence alignment