Detailed information NTG0224928

Bioinformatically predicted

Overview

• Name: comGA
• Type: Machinery gene
• Locus tag: BSR25_RS08760
• Genome accession: NZ_CP020604
• Coordinates: 1717962..1718900 (-)
• Length: 312 a.a.
• NCBI ID: WP_010906320.1
• Uniprot ID: A0AAC9W673
• Organism: Lactococcus lactis subsp. lactis bv. diacetylactis strain FM03
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1712962..1723900

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BSR25_RS08730 (BSR25_1728)	-	1713139..1713348 (-)	210	WP_023349177.1	helix-turn-helix transcriptional regulator	-
BSR25_RS08735 (BSR25_1729)	-	1713539..1713961 (+)	423	WP_023349178.1	helix-turn-helix domain-containing protein	-
BSR25_RS08740 (BSR25_1730)	-	1713972..1714556 (+)	585	WP_023349179.1	hypothetical protein	-
BSR25_RS08745 (BSR25_1731)	-	1714610..1715272 (+)	663	WP_023349180.1	SHOCT domain-containing protein	-
BSR25_RS08750 (BSR25_1732)	-	1715387..1716844 (+)	1458	WP_058147724.1	recombinase family protein	-
BSR25_RS12990	-	1716841..1716981 (-)	141	WP_228777719.1	hypothetical protein	-
BSR25_RS08755 (BSR25_1733)	comGB	1716995..1718068 (-)	1074	WP_010906319.1	competence type IV pilus assembly protein ComGB	Machinery gene
BSR25_RS08760 (BSR25_1734)	comGA	1717962..1718900 (-)	939	WP_010906320.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 312 a.a.
• Molecular weight: 35719.89 Da
• Isoelectric Point: 6.1804

>NTDB_id=224928 BSR25_RS08760 WP_010906320.1 1717962..1718900(-) (comGA) [Lactococcus lactis subsp. lactis bv. diacetylactis strain FM03]
MIPKMAKELIQRAIDRDASDIYLIASKEKYKLYFRQMTARDLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
IEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKELDFWFDQELENYDCKRGLHLFAGPVGSGKTSLMFDLARRHFTDLQV
INIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHASSVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTPTEAKREKIKTD

Nucleotide

• Length: 939 bp

>NTDB_id=224928 BSR25_RS08760 WP_010906320.1 1717962..1718900(-) (comGA) [Lactococcus lactis subsp. lactis bv. diacetylactis strain FM03]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAGAGCAATTGATAGAGATGCATCAGATATTTATCTGATTGCTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCACGTGATCTGGTAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAATTGGGAGCTTGTTGGTATGAA
ATAGAGGAAAGTAATGCAAAACGTTTGCGTCTTTCGACAGTGGGGGATTTCGAGGGTAATGAATCTTTAGTAATACGTCT
CTTGCATGATCAAAACAAAGAACTTGATTTTTGGTTTGATCAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCCGGTCCAGTAGGCTCGGGAAAAACATCTTTAATGTTTGATTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCAATATAGAAGAACCCGTTGAGTTGATTGATTCCGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTGTTTTCAACCGTTCATGCCTCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGACATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACACCTACTGAAGCCAAAAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (9-265)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGA	Lactococcus lactis subsp. cremoris KW2	76.603	100	0.766
comGA/cglA	Streptococcus sobrinus strain NIDR 6715-7	54.662	99.679	0.545
comGA/cglA/cilD	Streptococcus mitis NCTC 12261	53.055	99.679	0.529
comGA/cglA/cilD	Streptococcus pneumoniae Rx1	52.09	99.679	0.519
comGA/cglA/cilD	Streptococcus pneumoniae D39	52.09	99.679	0.519
comGA/cglA/cilD	Streptococcus pneumoniae R6	52.09	99.679	0.519
comGA/cglA/cilD	Streptococcus pneumoniae TIGR4	52.09	99.679	0.519
comYA	Streptococcus gordonii str. Challis substr. CH1	51.923	100	0.519
comYA	Streptococcus mutans UA140	51.923	100	0.519
comYA	Streptococcus mutans UA159	51.923	100	0.519