Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   BSR25_RS08755 Genome accession   NZ_CP020604
Coordinates   1716995..1718068 (-) Length   357 a.a.
NCBI ID   WP_010906319.1    Uniprot ID   A0A552YT77
Organism   Lactococcus lactis subsp. lactis bv. diacetylactis strain FM03     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1711995..1723068
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSR25_RS08720 (BSR25_1725) - 1712512..1712760 (-) 249 WP_014570815.1 hypothetical protein -
  BSR25_RS13510 (BSR25_1726) - 1712774..1712896 (-) 123 WP_023164646.1 hypothetical protein -
  BSR25_RS08725 (BSR25_1727) - 1712893..1713075 (-) 183 WP_023349176.1 hypothetical protein -
  BSR25_RS08730 (BSR25_1728) - 1713139..1713348 (-) 210 WP_023349177.1 helix-turn-helix transcriptional regulator -
  BSR25_RS08735 (BSR25_1729) - 1713539..1713961 (+) 423 WP_023349178.1 helix-turn-helix domain-containing protein -
  BSR25_RS08740 (BSR25_1730) - 1713972..1714556 (+) 585 WP_023349179.1 hypothetical protein -
  BSR25_RS08745 (BSR25_1731) - 1714610..1715272 (+) 663 WP_023349180.1 SHOCT domain-containing protein -
  BSR25_RS08750 (BSR25_1732) - 1715387..1716844 (+) 1458 WP_058147724.1 recombinase family protein -
  BSR25_RS12990 - 1716841..1716981 (-) 141 WP_228777719.1 hypothetical protein -
  BSR25_RS08755 (BSR25_1733) comGB 1716995..1718068 (-) 1074 WP_010906319.1 competence type IV pilus assembly protein ComGB Machinery gene
  BSR25_RS08760 (BSR25_1734) comGA 1717962..1718900 (-) 939 WP_010906320.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 40330.51 Da        Isoelectric Point: 10.3000

>NTDB_id=224927 BSR25_RS08755 WP_010906319.1 1716995..1718068(-) (comGB) [Lactococcus lactis subsp. lactis bv. diacetylactis strain FM03]
MSLGHQKIGIKKLISYLQMDISHLLKPKGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKEFTLKMKEGLA
SGQALSELLESLSFSKNVVTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEQFFTKID
RLMQLIQPLVFIFVALMIILLYAAMLLPIYSNMGSGI

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=224927 BSR25_RS08755 WP_010906319.1 1716995..1718068(-) (comGB) [Lactococcus lactis subsp. lactis bv. diacetylactis strain FM03]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACACCTACTGAAGCC
AAAAGGGAAAAAATTAAGACTGATTAAACAAGCCAAACTTATCCAACTGATGGGAAATCTTTTGAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAGAATTTACATTAAAAATGAAAGAAGGTCTCGCT
TCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAAGT
GCATGGTAACTTGTCAGGGACGATGCAATTAGTTGAACTTCATCTAAAAAAACAGCTTAAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGGGCTTAAAAAATTATTTACTACCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAATTTTACATTTCTTGTTAAATTACCTC
TGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATCTAATTGCTCAAGGTGTTGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAAGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCGTTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTTGGGAACAATTTTTTACAAAGATAGAT
CGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATTATTTTGCTATATGCAGCCATGTTACT
CCCCATCTATTCAAATATGGGTTCGGGAATATAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-348)

Type II secretion system (T2SS), protein F (T2SSF)

(41-159)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

83.578

95.518

0.798
  comYB Streptococcus gordonii str. Challis substr. CH1

53.959

95.518

0.515
  comYB Streptococcus mutans UA140

53.372

95.518

0.51
  comYB Streptococcus mutans UA159

53.079

95.518

0.507
  comGB/cglB Streptococcus mitis SK321

51.343

93.838

0.482
  comGB/cglB Streptococcus pneumoniae TIGR4

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae Rx1

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae D39

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae R6

51.045

93.838

0.479
  comGB/cglB Streptococcus mitis NCTC 12261

50.448

93.838

0.473

Multiple sequence alignment