Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   B7D75_RS23300 Genome accession   NZ_CP020560
Coordinates   4920210..4921427 (+) Length   405 a.a.
NCBI ID   WP_107114466.1    Uniprot ID   -
Organism   Pseudomonas paraeruginosa strain Cr1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4915210..4926427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7D75_RS23275 (B7D75_23290) nadC 4915312..4916160 (+) 849 WP_003152758.1 carboxylating nicotinate-nucleotide diphosphorylase -
  B7D75_RS23285 (B7D75_23300) - 4916341..4917738 (-) 1398 WP_234600078.1 O-antigen ligase -
  B7D75_RS23290 (B7D75_23305) - 4917812..4918276 (-) 465 WP_058175466.1 pilin -
  B7D75_RS23295 (B7D75_23310) pilB 4918507..4920206 (+) 1700 Protein_4535 type IV-A pilus assembly ATPase PilB -
  B7D75_RS23300 (B7D75_23315) pilC 4920210..4921427 (+) 1218 WP_107114466.1 type II secretion system F family protein Machinery gene
  B7D75_RS23305 (B7D75_23320) pilD 4921428..4922300 (+) 873 WP_107114467.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  B7D75_RS23310 (B7D75_23325) coaE 4922297..4922908 (+) 612 WP_107114468.1 dephospho-CoA kinase -
  B7D75_RS23315 (B7D75_23330) yacG 4922905..4923105 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  B7D75_RS23320 (B7D75_23335) - 4923143..4923352 (-) 210 WP_033998211.1 hypothetical protein -
  B7D75_RS23325 (B7D75_23340) - 4923458..4924147 (-) 690 WP_012077298.1 energy-coupling factor ABC transporter permease -
  B7D75_RS23330 (B7D75_23345) - 4924144..4924614 (-) 471 WP_107114469.1 FAD/FMN-containing dehydrogenase -
  B7D75_RS23335 (B7D75_23350) - 4924611..4925036 (-) 426 WP_003152776.1 GNAT family N-acetyltransferase -
  B7D75_RS23340 (B7D75_23355) - 4925168..4925797 (+) 630 WP_033998217.1 DUF1780 domain-containing protein -
  B7D75_RS23345 (B7D75_23360) - 4925794..4926234 (+) 441 WP_107114470.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43968.78 Da        Isoelectric Point: 9.8103

>NTDB_id=224129 B7D75_RS23300 WP_107114466.1 4920210..4921427(+) (pilC) [Pseudomonas paraeruginosa strain Cr1]
MADKALKTSVFVWEGTDKKGTKVKGELSSQNPTLVKAQLRKQGITPVKVRKKGISLLGAGKKIKPMDIALFTRQMSTMMA
AGVPLLQSFDIISEGFDNPNMRKLVEEIKQEVAGGNSLANSLRKKPQYFDSLYCNLVDAGEQSGALETLLDRVAAYKEKT
EALKAKIKKAMTYPIAVIVVAIIVSAILLIKVVPQFQSVFEGFGAELPAFTQMVINISNVLQEWWLLVLLMMGGAGFLLN
HAYKRSEKFRDATDRTVLKLPIVGAILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRDAVGKIKQDVSTGM
QLNFSMRTTNIFPSMAIQMTAIGEESGALDDMLAKVAGFYEQEVDNAVDNLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=224129 B7D75_RS23300 WP_107114466.1 4920210..4921427(+) (pilC) [Pseudomonas paraeruginosa strain Cr1]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCGTATGGGAAGGCACTGACAAGAAAGGCACCAAGGTCAAGGGCGAGCT
GTCCAGCCAGAACCCGACCCTGGTCAAGGCGCAACTGCGCAAGCAAGGCATCACGCCAGTCAAGGTCCGCAAGAAAGGCA
TTTCCCTGCTGGGGGCGGGGAAGAAGATCAAGCCCATGGACATCGCCCTGTTCACCCGTCAGATGTCCACCATGATGGCC
GCCGGCGTGCCACTGCTACAGTCTTTCGACATCATCAGCGAAGGCTTCGACAATCCGAACATGCGCAAGCTGGTGGAGGA
GATCAAGCAGGAAGTCGCCGGCGGTAACAGCCTGGCCAACTCGCTGCGCAAGAAGCCGCAGTACTTCGACTCGCTCTACT
GCAACCTGGTGGACGCCGGCGAACAATCCGGCGCCCTGGAAACCCTGCTCGACCGGGTCGCCGCCTATAAGGAAAAGACC
GAGGCGCTGAAGGCCAAGATCAAGAAGGCCATGACCTACCCGATCGCGGTGATCGTCGTCGCCATCATCGTCTCGGCGAT
CCTACTGATCAAGGTGGTACCGCAGTTCCAGTCGGTGTTCGAGGGCTTCGGCGCCGAACTGCCGGCTTTCACCCAAATGG
TCATCAATATCTCCAACGTGCTCCAAGAATGGTGGCTCCTGGTACTGCTGATGATGGGCGGCGCCGGCTTCCTGCTGAAC
CACGCCTACAAGCGTTCGGAGAAGTTCCGCGACGCCACCGACCGCACGGTACTGAAGCTGCCGATCGTCGGCGCCATCCT
CTACAAATCCGCCGTGGCACGCTACGCGCGGACGCTGTCGACCACCTTCGCCGCCGGCGTGCCATTGGTGGAAGCCCTCG
ACTCGGTTTCCGGCGCCACCGGTAACGTGGTGTTCAGGGACGCGGTGGGCAAGATCAAGCAGGACGTGTCCACCGGCATG
CAGTTGAACTTCTCCATGCGCACCACCAACATCTTCCCCAGCATGGCCATCCAGATGACCGCCATCGGCGAGGAATCGGG
CGCGCTGGACGACATGCTGGCCAAGGTAGCTGGCTTCTACGAGCAGGAAGTGGATAATGCCGTGGACAACCTGACCGCCC
TGATGGAACCGATGATCATGGCGGTCCTCGGGGTTCTGGTCGGTGGCCTGATCATCGCCATGTACCTGCCGATCTTCCAG
CTGGGCAACGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

78.519

100

0.785
  pilC Acinetobacter baylyi ADP1

61.275

100

0.617
  pilC Acinetobacter baumannii D1279779

60.644

99.753

0.605
  pilC Legionella pneumophila strain ERS1305867

54.04

97.778

0.528
  pilG Neisseria gonorrhoeae MS11

46.287

99.753

0.462
  pilG Neisseria meningitidis 44/76-A

45.771

99.259

0.454
  pilC Vibrio cholerae strain A1552

41.481

100

0.415
  pilC Vibrio campbellii strain DS40M4

40.648

99.012

0.402
  pilC Thermus thermophilus HB27

38.155

99.012

0.378

Multiple sequence alignment