Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   B5D82_RS00475 Genome accession   NZ_CP020465
Coordinates   108071..109327 (-) Length   418 a.a.
NCBI ID   WP_081148338.1    Uniprot ID   A0A222G3K4
Organism   Cognaticolwellia beringensis strain NB097-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 103071..114327
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5D82_RS00450 (B5D82_00450) mutT 104474..104869 (+) 396 WP_081148332.1 8-oxo-dGTP diphosphatase MutT -
  B5D82_RS00455 (B5D82_00455) yacG 105014..105250 (-) 237 WP_081148334.1 DNA gyrase inhibitor YacG -
  B5D82_RS00460 (B5D82_00460) zapD 105434..106183 (-) 750 WP_081148336.1 cell division protein ZapD -
  B5D82_RS00465 (B5D82_00465) coaE 106276..106887 (-) 612 WP_081154211.1 dephospho-CoA kinase -
  B5D82_RS00470 (B5D82_00470) pilD 107093..107953 (-) 861 WP_081154213.1 A24 family peptidase Machinery gene
  B5D82_RS00475 (B5D82_00475) pilC 108071..109327 (-) 1257 WP_081148338.1 type II secretion system F family protein Machinery gene
  B5D82_RS00480 (B5D82_00480) pilB 109487..111196 (-) 1710 WP_081148340.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B5D82_RS00485 (B5D82_00485) - 111341..111769 (-) 429 WP_281255936.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  B5D82_RS00490 (B5D82_00490) nadC 112274..113161 (-) 888 WP_081148342.1 carboxylating nicotinate-nucleotide diphosphorylase -
  B5D82_RS00495 (B5D82_00495) - 113162..113683 (-) 522 WP_081148344.1 retropepsin-like aspartic protease -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 45368.61 Da        Isoelectric Point: 9.5165

>NTDB_id=223445 B5D82_RS00475 WP_081148338.1 108071..109327(-) (pilC) [Cognaticolwellia beringensis strain NB097-1]
MAVSSTTKVKDKKSKVKALDVYKWQGLNRKGKKISGELNATSMLELKAQLRKQGITPGKISKKAKPLFGMSGDKKILPVD
IAVLTRQIATMLGAGVPLVQTIEMIGAGHNNANMQKLLGTIGNKLQSGIPLSECLREHPKYFDDLYCDLVNSGEQSGSLE
TIYDRIAVYKEKAEVLKAKIKKAMTYPISVLVIALIVTSVLLIFVVPVFKDIFDSFGAELPGFTLLVLAISDFMQAYWYF
GLAGIFLSFYLFKKAHANSQNFRDSVDKKILKVPVVGDVLKKAAVARYARTLSTTFAAGVPLPDALESAAGASGNAVFRD
AILEIRAEVTSGMQMNLAMRNCAIFPDMVIQMVAIGEESGAVDDMLAKVATVYEQEVDNAVDNLTTLLEPMIMAVLGVVI
GGLIIAMYLPIFQIGMVV

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=223445 B5D82_RS00475 WP_081148338.1 108071..109327(-) (pilC) [Cognaticolwellia beringensis strain NB097-1]
ATGGCTGTTTCTTCAACAACAAAAGTAAAAGACAAGAAAAGTAAAGTTAAAGCGCTTGATGTTTATAAATGGCAAGGGCT
GAACCGTAAAGGTAAAAAAATAAGTGGTGAGCTTAATGCCACCAGTATGTTGGAGCTAAAAGCCCAACTGCGTAAGCAAG
GTATCACCCCAGGTAAAATCAGTAAAAAAGCTAAACCTTTATTTGGCATGAGCGGCGATAAAAAAATTCTTCCGGTTGAT
ATCGCGGTACTTACTCGCCAAATTGCCACTATGCTAGGCGCAGGTGTACCTTTAGTGCAAACCATTGAAATGATCGGCGC
TGGCCACAACAATGCCAATATGCAGAAATTACTTGGCACTATTGGCAACAAACTGCAATCGGGTATCCCGCTTTCTGAAT
GCTTGCGTGAACACCCTAAATATTTTGATGATTTATATTGCGACTTGGTTAACTCTGGTGAGCAATCTGGTTCATTAGAA
ACCATATATGATCGTATTGCTGTCTATAAAGAAAAAGCCGAAGTATTGAAAGCAAAAATAAAAAAAGCCATGACCTATCC
TATTTCTGTGTTAGTTATCGCCTTAATTGTTACCTCCGTCTTATTAATATTTGTAGTACCCGTATTTAAAGATATTTTTG
ATAGCTTTGGCGCCGAGCTCCCAGGTTTTACGTTATTAGTGTTAGCCATATCTGATTTTATGCAAGCTTATTGGTATTTT
GGCTTAGCTGGTATATTCCTCAGTTTTTACCTTTTCAAAAAAGCGCATGCCAATAGCCAGAACTTTCGTGACAGTGTTGA
TAAAAAAATATTAAAAGTACCTGTTGTTGGTGATGTATTAAAAAAAGCGGCGGTTGCCCGTTATGCCAGAACACTTTCAA
CAACGTTTGCTGCTGGTGTGCCGTTGCCTGATGCTCTAGAGTCAGCCGCAGGTGCTTCAGGCAATGCAGTTTTTCGTGAT
GCTATTTTAGAAATACGCGCTGAAGTAACCTCTGGTATGCAAATGAATTTAGCCATGAGAAACTGTGCTATTTTCCCCGA
TATGGTGATTCAAATGGTGGCTATTGGTGAAGAGTCTGGCGCGGTTGACGATATGCTCGCCAAAGTAGCGACTGTCTATG
AGCAAGAAGTAGATAACGCCGTAGATAACTTAACTACCCTGTTAGAGCCAATGATAATGGCGGTATTAGGCGTGGTTATT
GGTGGCTTGATCATCGCCATGTATTTACCAATATTCCAAATAGGTATGGTGGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(85-207)

Type II secretion system (T2SS), protein F (T2SSF)

(289-410)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A222G3K4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

61.558

95.215

0.586
  pilC Acinetobacter baylyi ADP1

54.208

96.651

0.524
  pilC Legionella pneumophila strain ERS1305867

52.311

98.325

0.514
  pilC Acinetobacter baumannii D1279779

52.723

96.651

0.51
  pilC Vibrio cholerae strain A1552

44.913

96.411

0.433
  pilC Vibrio campbellii strain DS40M4

43.97

95.215

0.419
  pilG Neisseria meningitidis 44/76-A

43.928

92.584

0.407
  pilG Neisseria gonorrhoeae MS11

43.928

92.584

0.407

Multiple sequence alignment