Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   B5D82_RS00470 Genome accession   NZ_CP020465
Coordinates   107093..107953 (-) Length   286 a.a.
NCBI ID   WP_081154213.1    Uniprot ID   A0A222GD73
Organism   Cognaticolwellia beringensis strain NB097-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 102093..112953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5D82_RS00445 (B5D82_00445) - 102864..104039 (+) 1176 WP_081148330.1 IS4 family transposase -
  B5D82_RS00450 (B5D82_00450) mutT 104474..104869 (+) 396 WP_081148332.1 8-oxo-dGTP diphosphatase MutT -
  B5D82_RS00455 (B5D82_00455) yacG 105014..105250 (-) 237 WP_081148334.1 DNA gyrase inhibitor YacG -
  B5D82_RS00460 (B5D82_00460) zapD 105434..106183 (-) 750 WP_081148336.1 cell division protein ZapD -
  B5D82_RS00465 (B5D82_00465) coaE 106276..106887 (-) 612 WP_081154211.1 dephospho-CoA kinase -
  B5D82_RS00470 (B5D82_00470) pilD 107093..107953 (-) 861 WP_081154213.1 A24 family peptidase Machinery gene
  B5D82_RS00475 (B5D82_00475) pilC 108071..109327 (-) 1257 WP_081148338.1 type II secretion system F family protein Machinery gene
  B5D82_RS00480 (B5D82_00480) pilB 109487..111196 (-) 1710 WP_081148340.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B5D82_RS00485 (B5D82_00485) - 111341..111769 (-) 429 WP_281255936.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 31841.93 Da        Isoelectric Point: 7.3448

>NTDB_id=223444 B5D82_RS00470 WP_081154213.1 107093..107953(-) (pilD) [Cognaticolwellia beringensis strain NB097-1]
MTHSPTYFYSIVFIFSLLIGSFLNVVIYRLPKMLEQGWKSECREFLADELAKPIQTDDSLITLSTPSSSCPSCQHKIRFY
ENIPLISWLFLRGKCSQCQAKISLRYPLVELATALLSVVIAAHYGVTFTTLMLLVLTWGLIALTLIDVDHMLLPDQITLP
LIWLGLLVNINAAIVPLSDAVIGAVAGYMSLFSIFWLFKLITGKDGMGHGDFKLVALFGAWLGWQLLPLLILMASAVGAI
IGISLMVFKNHQREQAIPFGPYLAIAGWITLLWGNGIWSWYLSSLT

Nucleotide


Download         Length: 861 bp        

>NTDB_id=223444 B5D82_RS00470 WP_081154213.1 107093..107953(-) (pilD) [Cognaticolwellia beringensis strain NB097-1]
ATGACACACTCCCCCACCTATTTCTACAGCATAGTGTTTATTTTTTCGCTACTGATTGGTAGCTTTTTAAACGTAGTTAT
TTATCGCTTACCTAAAATGTTAGAGCAAGGCTGGAAAAGTGAATGCCGTGAATTTTTAGCTGATGAGTTAGCAAAACCCA
TACAAACTGACGATAGCCTGATCACCCTTTCAACGCCAAGTTCTAGCTGCCCGTCATGCCAACATAAAATTCGTTTCTAT
GAAAACATACCGCTAATAAGTTGGTTGTTTTTACGCGGGAAATGTAGCCAATGTCAGGCAAAAATATCACTACGCTACCC
ATTAGTTGAGTTAGCAACCGCGTTATTAAGTGTGGTGATCGCGGCCCATTACGGCGTTACCTTCACTACGCTTATGCTAT
TAGTGCTAACTTGGGGTTTAATTGCTTTAACCTTAATTGATGTAGACCATATGTTATTGCCTGACCAAATAACGCTGCCA
TTAATATGGTTAGGGCTTTTGGTGAATATTAATGCTGCCATTGTACCGTTAAGCGACGCAGTTATTGGCGCTGTTGCCGG
TTACATGAGCTTGTTCAGTATATTTTGGCTGTTTAAATTAATTACCGGTAAAGACGGTATGGGCCATGGCGACTTTAAGT
TAGTTGCGCTATTTGGTGCCTGGTTGGGTTGGCAGTTACTGCCGTTATTAATTTTAATGGCATCAGCCGTTGGCGCGATT
ATTGGCATTAGTTTAATGGTCTTTAAGAACCATCAGCGTGAACAAGCCATTCCTTTTGGCCCATATTTAGCTATTGCAGG
TTGGATAACCCTGCTTTGGGGTAACGGAATTTGGTCTTGGTATTTAAGTTCGTTAACTTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(134-243)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(14-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A222GD73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

53.521

99.301

0.531
  pilD Vibrio campbellii strain DS40M4

52.982

99.65

0.528
  pilD Acinetobacter baumannii D1279779

52.688

97.552

0.514
  pilD Acinetobacter nosocomialis M2

52.688

97.552

0.514
  pilD Neisseria gonorrhoeae MS11

48.175

95.804

0.462

Multiple sequence alignment