Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   B5D82_RS00480 Genome accession   NZ_CP020465
Coordinates   109487..111196 (-) Length   569 a.a.
NCBI ID   WP_081148340.1    Uniprot ID   A0A222G3N1
Organism   Cognaticolwellia beringensis strain NB097-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 104487..116196
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5D82_RS00455 (B5D82_00455) yacG 105014..105250 (-) 237 WP_081148334.1 DNA gyrase inhibitor YacG -
  B5D82_RS00460 (B5D82_00460) zapD 105434..106183 (-) 750 WP_081148336.1 cell division protein ZapD -
  B5D82_RS00465 (B5D82_00465) coaE 106276..106887 (-) 612 WP_081154211.1 dephospho-CoA kinase -
  B5D82_RS00470 (B5D82_00470) pilD 107093..107953 (-) 861 WP_081154213.1 A24 family peptidase Machinery gene
  B5D82_RS00475 (B5D82_00475) pilC 108071..109327 (-) 1257 WP_081148338.1 type II secretion system F family protein Machinery gene
  B5D82_RS00480 (B5D82_00480) pilB 109487..111196 (-) 1710 WP_081148340.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B5D82_RS00485 (B5D82_00485) - 111341..111769 (-) 429 WP_281255936.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  B5D82_RS00490 (B5D82_00490) nadC 112274..113161 (-) 888 WP_081148342.1 carboxylating nicotinate-nucleotide diphosphorylase -
  B5D82_RS00495 (B5D82_00495) - 113162..113683 (-) 522 WP_081148344.1 retropepsin-like aspartic protease -
  B5D82_RS00500 (B5D82_00500) ampD 113826..114407 (+) 582 WP_081148346.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  B5D82_RS00505 (B5D82_00505) ampE 114616..115494 (+) 879 WP_081148348.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62515.75 Da        Isoelectric Point: 4.9446

>NTDB_id=223446 B5D82_RS00480 WP_081148340.1 109487..111196(-) (pilB) [Cognaticolwellia beringensis strain NB097-1]
MKGLHQQSSLLSALTKHNVVPSDIAEKISADFIQQNKPFIRFLVEDKSINADDIASTLCRSFGCPLIDLTNFDTSLVPEG
VRNEKLIRKHQALPLYLRGKVLFVAMSDPTNLDALEEVQFNTGYSTELVIANETALAACIEKILEQDSDALDISDMDAEE
LSGIDVQEERKEEDNIGAGGDKEDAPIVVYINKILLDAIRKGASDLHFEPYEKSYRIRFRIDGILNEVAKPPVALASRMA
ARLKVMSKLDIAERRVPQDGRIKLALSKKKSIDFRVSTLPTMWGEKIVMRILDSSSAMLGIDMLGYAPEQKKIYMDALAQ
PQGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLDGINQVQINVRAGLTFPGALRSFLRQDPDIVMVGEI
RDLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPSYNVASSVTIIIAQRLARRLCVQCREEEHIPEAELIAQG
FLPAQLENIKLFKPVGCDFCTGGYKGRVGIYEVIKISDVTANLIMTGSNSLDIAAQCQREGYNNLRQSGLEKAALGVTSL
EEVNRVTNS

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=223446 B5D82_RS00480 WP_081148340.1 109487..111196(-) (pilB) [Cognaticolwellia beringensis strain NB097-1]
ATGAAGGGACTTCACCAACAATCAAGCCTACTTTCTGCGTTAACTAAACATAATGTTGTACCTAGCGATATAGCGGAAAA
AATTAGTGCTGATTTTATTCAACAAAACAAACCTTTTATACGGTTTTTAGTTGAAGATAAAAGCATTAATGCCGACGACA
TTGCCAGCACGCTGTGTCGTAGTTTTGGCTGTCCGCTAATTGATTTAACTAATTTTGATACTAGCTTGGTACCTGAAGGT
GTCCGTAACGAAAAACTGATCCGTAAACACCAAGCCTTGCCGTTATATTTACGTGGCAAAGTCTTATTCGTTGCTATGTC
AGACCCGACTAATCTTGATGCCCTTGAAGAAGTTCAGTTTAATACCGGTTACTCTACCGAATTGGTTATTGCCAACGAAA
CCGCGTTAGCTGCCTGTATTGAAAAAATATTAGAACAAGACAGCGATGCCCTTGATATTTCCGATATGGACGCGGAGGAA
TTATCAGGTATTGATGTTCAAGAAGAACGCAAAGAAGAAGATAATATTGGCGCTGGCGGTGATAAAGAAGACGCCCCAAT
CGTTGTTTATATTAATAAAATATTACTCGACGCCATTCGCAAAGGCGCGTCTGATTTACACTTTGAACCTTACGAGAAGT
CTTATCGTATTCGTTTTCGTATTGACGGTATATTAAACGAAGTAGCTAAACCGCCCGTAGCGTTAGCCTCGCGCATGGCG
GCACGTTTAAAAGTCATGTCGAAGTTAGACATTGCTGAACGCCGTGTTCCACAAGATGGCCGAATTAAATTAGCCTTATC
GAAAAAGAAGTCGATTGATTTTCGTGTTAGTACCCTGCCCACCATGTGGGGCGAAAAAATAGTAATGCGTATTCTTGACT
CGTCTAGTGCCATGCTAGGTATTGATATGCTGGGCTATGCGCCAGAGCAAAAGAAAATATACATGGACGCTCTAGCACAA
CCACAAGGGATGATTTTAGTTACCGGCCCAACGGGTTCAGGTAAAACCGTGTCACTTTATACCGGGCTTAATATTTTAAA
TACCGAAGAGCGTAATATTTCAACCGCGGAAGATCCGGTTGAAATTAATCTCGATGGAATTAACCAAGTACAAATAAATG
TTCGTGCAGGTTTAACCTTCCCAGGCGCATTACGCTCATTCCTCCGACAAGATCCCGATATTGTTATGGTAGGTGAGATT
CGTGATTTAGAAACCGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCATTTAGTGTTATCAACATTACATACCAACTC
GGCAGCCGAAACACTAACCCGTTTATTAAATATGGGCGTGCCGTCATACAACGTTGCCAGCTCGGTGACTATTATTATCG
CCCAGCGATTAGCAAGGCGCTTATGTGTTCAGTGTCGAGAAGAAGAACACATACCTGAAGCCGAACTCATTGCCCAAGGT
TTTTTACCTGCACAACTAGAAAATATTAAATTATTTAAACCTGTCGGGTGCGATTTTTGTACCGGCGGTTATAAAGGCCG
TGTCGGTATCTACGAAGTCATAAAAATTAGTGATGTTACCGCAAACTTAATTATGACGGGCTCAAACTCGTTAGATATTG
CCGCACAATGCCAACGCGAAGGTTATAATAATTTACGCCAGTCTGGCTTAGAAAAAGCCGCATTAGGCGTAACCAGTTTA
GAAGAAGTTAACCGGGTAACTAATTCTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A222G3N1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.023

93.497

0.552
  pilB Acinetobacter baumannii D1279779

58.473

94.376

0.552
  pilB Legionella pneumophila strain ERS1305867

56.697

95.782

0.543
  pilB Vibrio cholerae strain A1552

53.774

93.146

0.501
  pilB Vibrio campbellii strain DS40M4

52.363

92.97

0.487
  pilB Vibrio parahaemolyticus RIMD 2210633

51.894

92.794

0.482
  pilF Neisseria gonorrhoeae MS11

48.49

98.946

0.48
  pilB/pilB1 Synechocystis sp. PCC 6803

40.039

90.861

0.364
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.547

94.376

0.364

Multiple sequence alignment