Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   A6J87_RS05485 Genome accession   NZ_CP020451
Coordinates   1082770..1083711 (+) Length   313 a.a.
NCBI ID   WP_080611681.1    Uniprot ID   -
Organism   Streptococcus salivarius strain FDAARGOS_259     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1077770..1088711
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6J87_RS05475 (A6J87_05465) rpoC 1078525..1082163 (+) 3639 WP_037603707.1 DNA-directed RNA polymerase subunit beta' -
  A6J87_RS05480 (A6J87_05470) - 1082326..1082688 (+) 363 WP_080611679.1 DUF1033 family protein -
  A6J87_RS05485 (A6J87_05475) comGA/cglA/cilD 1082770..1083711 (+) 942 WP_080611681.1 competence type IV pilus ATPase ComGA Machinery gene
  A6J87_RS05490 (A6J87_05480) comYB 1083593..1084693 (+) 1101 WP_125871313.1 competence type IV pilus assembly protein ComGB Machinery gene
  A6J87_RS10985 (A6J87_10795) - 1084690..1084758 (+) 69 Protein_1025 competence protein ComGC -
  A6J87_RS05500 (A6J87_05485) - 1084770..1086260 (-) 1491 WP_225791454.1 recombinase family protein -
  A6J87_RS05505 (A6J87_05490) - 1086357..1086962 (-) 606 WP_225791455.1 hypothetical protein -
  A6J87_RS05510 (A6J87_05495) - 1086956..1087333 (-) 378 WP_080611682.1 ImmA/IrrE family metallo-endopeptidase -
  A6J87_RS05515 (A6J87_05500) - 1087340..1087705 (-) 366 WP_080611684.1 helix-turn-helix domain-containing protein -
  A6J87_RS05520 (A6J87_05505) - 1087874..1088077 (+) 204 WP_080611685.1 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35232.28 Da        Isoelectric Point: 5.4454

>NTDB_id=223250 A6J87_RS05485 WP_080611681.1 1082770..1083711(+) (comGA/cglA/cilD) [Streptococcus salivarius strain FDAARGOS_259]
MVTEFAKEMIKNADSCGAQDIYVIPRQDNYELYMRVGQERRLIDVYRPDFMASLIGHFKFVAGMMVGEKRRSQLGSCDYD
CGDGQRVSLRLSTVGDYRGLESLVIRVLHSERRDLVYWNQGIQLIMDALDYRGLYLFAGPVGSGKTTLMHELVQECFKGQ
QVISIEDPVEIKQDNVLQLQVNQAIDMTYDNLIKLSLRHRPDVLIIGEIRDKETARAVIRASLTGVTVLSTIHAKSVAGV
YERLLDLGVDKSELDNALQGIAYMRLIKGGGVIDFASENFQSHSSTSWNQQLEGLVQQGYLTEGDIKGEKIKD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=223250 A6J87_RS05485 WP_080611681.1 1082770..1083711(+) (comGA/cglA/cilD) [Streptococcus salivarius strain FDAARGOS_259]
ATGGTAACAGAATTTGCTAAGGAAATGATCAAAAATGCTGATAGTTGTGGGGCTCAAGACATCTATGTCATTCCACGTCA
GGATAATTACGAGCTCTATATGCGAGTTGGCCAGGAGAGGAGATTAATTGATGTATATCGGCCTGATTTCATGGCTAGTC
TTATTGGTCACTTTAAATTTGTGGCGGGAATGATGGTGGGTGAGAAGCGTAGGAGTCAACTAGGTTCCTGTGACTATGAT
TGTGGTGATGGTCAAAGGGTTTCTCTGCGTTTATCAACTGTTGGGGATTATCGCGGCTTAGAAAGTTTAGTTATTCGTGT
TCTACATTCCGAACGTCGAGACTTGGTGTATTGGAATCAAGGAATCCAGCTGATTATGGATGCTTTGGATTATCGTGGCT
TGTATCTCTTTGCAGGTCCCGTAGGTTCTGGAAAGACTACGCTTATGCATGAATTGGTTCAGGAGTGTTTTAAGGGACAG
CAGGTGATTTCGATTGAAGATCCTGTGGAAATTAAACAGGATAATGTCTTGCAACTCCAGGTAAATCAGGCAATTGACAT
GACCTATGATAATTTGATTAAGCTATCACTACGTCACCGCCCGGATGTTTTGATTATTGGAGAAATTCGAGATAAGGAGA
CTGCTCGAGCGGTTATTAGAGCTAGTCTGACAGGGGTGACTGTTCTTTCAACTATTCACGCAAAGAGTGTGGCAGGTGTT
TATGAGCGTCTTCTGGACCTCGGTGTAGATAAGTCTGAGTTGGATAATGCTCTTCAAGGGATTGCCTATATGCGTTTGAT
TAAGGGAGGAGGTGTGATTGATTTTGCCAGTGAAAATTTCCAAAGCCATTCGTCAACCAGCTGGAACCAACAGTTGGAAG
GCTTGGTTCAACAAGGATATCTCACTGAAGGGGATATCAAAGGGGAAAAAATTAAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.916

99.361

0.655
  comYA Streptococcus mutans UA159

65.176

100

0.652
  comYA Streptococcus mutans UA140

65.176

100

0.652
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

64.63

99.361

0.642
  comGA/cglA/cilD Streptococcus pneumoniae R6

64.63

99.361

0.642
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

64.63

99.361

0.642
  comGA/cglA/cilD Streptococcus pneumoniae D39

64.63

99.361

0.642
  comYA Streptococcus gordonii str. Challis substr. CH1

62.62

100

0.626
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.577

99.681

0.604
  comGA Lactococcus lactis subsp. cremoris KW2

51.125

99.361

0.508
  comGA Latilactobacillus sakei subsp. sakei 23K

41.392

87.22

0.361

Multiple sequence alignment