Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A6J38_RS08735 Genome accession   NZ_CP020411
Coordinates   1755473..1756867 (+) Length   464 a.a.
NCBI ID   WP_005694368.1    Uniprot ID   -
Organism   Haemophilus influenzae strain FDAARGOS_199     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1750473..1761867
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6J38_RS08710 (A6J38_08715) rsmE 1750486..1751223 (-) 738 WP_005694361.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  A6J38_RS08715 (A6J38_08720) lnt 1751273..1752802 (-) 1530 WP_010868973.1 apolipoprotein N-acyltransferase -
  A6J38_RS08720 (A6J38_08725) corC 1752825..1753724 (-) 900 WP_005694364.1 CNNM family magnesium/cobalt transport protein CorC -
  A6J38_RS08725 (A6J38_08730) ampD 1754357..1754911 (-) 555 WP_005694366.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  A6J38_RS08730 (A6J38_08735) pilA 1755027..1755476 (+) 450 WP_005694367.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  A6J38_RS08735 (A6J38_08740) pilB 1755473..1756867 (+) 1395 WP_005694368.1 GspE/PulE family protein Machinery gene
  A6J38_RS08740 (A6J38_08745) pilC 1756864..1758084 (+) 1221 WP_005694369.1 type II secretion system F family protein Machinery gene
  A6J38_RS08745 (A6J38_08750) pilD 1758081..1758773 (+) 693 WP_005694370.1 A24 family peptidase Machinery gene
  A6J38_RS08750 (A6J38_08755) rho 1758827..1760089 (-) 1263 WP_005648966.1 transcription termination factor Rho -
  A6J38_RS08755 (A6J38_08760) metJ 1760336..1760653 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  A6J38_RS08760 (A6J38_08765) cueR 1760667..1761053 (-) 387 WP_005654127.1 Cu(I)-responsive transcriptional regulator -
  A6J38_RS08765 (A6J38_08770) - 1761130..1761336 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  A6J38_RS08770 (A6J38_08775) - 1761411..1761617 (+) 207 WP_005656255.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52983.51 Da        Isoelectric Point: 6.0235

>NTDB_id=222415 A6J38_RS08735 WP_005694368.1 1755473..1756867(+) (pilB) [Haemophilus influenzae strain FDAARGOS_199]
MTSYALLHTQRVTAKNGEVFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPNQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
MTENQQQAFQRVLSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFNRLLR
TFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIKNSLLLVIAQRLVRKLCSKC
GGNLANSCDCHQGYRGRIGVYQFLHWQQNDYQTDFKNLRASGLEKVSQGITDEKEIERVLGKNL

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=222415 A6J38_RS08735 WP_005694368.1 1755473..1756867(+) (pilB) [Haemophilus influenzae strain FDAARGOS_199]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTAAAAATGGCGAAGTATTTACGATCTCGCCAGATTTATG
GGAACGCAATCAGCAGCAACAATCCTTGCTCTTACGTTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTCTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCCTGTGAAACCATTGCGTTTATAACGGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGAACCAAATGCAAGTGGAAGAACAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAATTTCAAGTGCGGTTTAGAATTGATGGAGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAGAAAATCGTGTTGCGAGCGCAACAAAATAAACCAGTAGAACTTAGCTTTTCTGAATTAGGT
ATGACAGAAAATCAGCAACAAGCATTTCAACGTGTGCTTAGCCAGCCACAAGGATTAATTTTAGTCACTGGCCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAATGGCTAAATACGCCTGATAAACATATTATGACTGCTGAAGATC
CCATTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAACCGCCTATTACGC
ACTTTTTTACGTCAAGATCCCGATATTATTATGCTAGGCGAAATTCGCGATGAAGAAAGTGCAATGATTGCATTACGTGC
GGCACAAACTGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCTATTTCTCGCTTACAACAACTCG
GTATTCAACAGTATGAAATTAAAAACAGTTTATTGCTCGTTATTGCACAGCGTCTTGTACGAAAGCTTTGTTCAAAGTGC
GGTGGAAATTTAGCAAATTCTTGTGATTGCCATCAAGGTTATCGAGGTCGAATCGGCGTGTATCAATTTCTACATTGGCA
GCAGAATGACTATCAAACGGATTTTAAAAATTTACGTGCGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACGGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAATTTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-98)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

100

100

1
  pilB Haemophilus influenzae 86-028NP

96.544

99.784

0.963
  pilB Glaesserella parasuis strain SC1401

58.093

97.198

0.565
  pilB Vibrio cholerae strain A1552

39.279

100

0.446
  pilB Vibrio campbellii strain DS40M4

40.412

100

0.422
  pilB Vibrio parahaemolyticus RIMD 2210633

40.373

100

0.42
  pilB Acinetobacter baylyi ADP1

39.113

100

0.418
  pilB Legionella pneumophila strain ERS1305867

39.264

100

0.414
  pilB Acinetobacter baumannii D1279779

39.709

100

0.412
  pilF Neisseria gonorrhoeae MS11

36.765

100

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.393

100

0.377
  pilF Thermus thermophilus HB27

36.134

100

0.371

Multiple sequence alignment