Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   B5U27_RS04570 Genome accession   NZ_CP020351
Coordinates   964245..965462 (-) Length   405 a.a.
NCBI ID   WP_005745480.1    Uniprot ID   A0AB37RAT5
Organism   Pseudomonas amygdali pv. lachrymans strain NM002     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 959245..970462
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5U27_RS04525 (B5U27_04525) - 959270..960568 (-) 1299 WP_005745472.1 NAD(P)/FAD-dependent oxidoreductase -
  B5U27_RS04530 (B5U27_04530) - 960665..960772 (-) 108 WP_080164415.1 DUF3094 family protein -
  B5U27_RS04535 (B5U27_04535) - 960786..961319 (-) 534 WP_005745473.1 MOSC domain-containing protein -
  B5U27_RS04540 (B5U27_04540) - 961316..961945 (-) 630 WP_003430735.1 DUF1780 domain-containing protein -
  B5U27_RS04545 (B5U27_04545) - 962060..962344 (+) 285 Protein_851 hypothetical protein -
  B5U27_RS04555 (B5U27_04555) yacG 962545..962754 (-) 210 WP_005745477.1 DNA gyrase inhibitor YacG -
  B5U27_RS04560 (B5U27_04560) coaE 962751..963374 (-) 624 WP_005745478.1 dephospho-CoA kinase -
  B5U27_RS04565 (B5U27_04565) pilD 963371..964243 (-) 873 WP_005745479.1 prepilin peptidase Machinery gene
  B5U27_RS04570 (B5U27_04570) pilC 964245..965462 (-) 1218 WP_005745480.1 type II secretion system F family protein Machinery gene
  B5U27_RS04575 (B5U27_04575) pilB 965465..967159 (-) 1695 WP_005745482.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B5U27_RS04580 (B5U27_04580) - 967385..967771 (+) 387 WP_005745483.1 pilin -
  B5U27_RS04585 (B5U27_04585) - 968117..969094 (+) 978 WP_007247761.1 IS5 family transposase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44029.95 Da        Isoelectric Point: 10.0788

>NTDB_id=221582 B5U27_RS04570 WP_005745480.1 964245..965462(-) (pilC) [Pseudomonas amygdali pv. lachrymans strain NM002]
MASKAVKVSVYTWEGVDKKGGKLSGEVSGHNLALVKAQLRKQGINFTKVRKKPVSIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIIAEGAENPNMRALVGSLKQEVSAGNSFATALRQKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPIAVLIVAIIVSGILLIKVVPQFQSVFAGFGAELPAFTLMVIGLSDIVQKWWLAIVGLFFVGAFLFK
RAYKQSEKFRDNIDRFLLKVPIIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFRDAVNKVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDNMLDKVATYYEDEVDNMVDNLTSLMEPMIMAVLGVIVGGLVIAMYLPIFK
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=221582 B5U27_RS04570 WP_005745480.1 964245..965462(-) (pilC) [Pseudomonas amygdali pv. lachrymans strain NM002]
ATGGCCAGCAAGGCAGTTAAAGTCAGTGTTTACACATGGGAAGGTGTGGACAAGAAAGGCGGCAAACTCAGTGGCGAAGT
GAGCGGGCACAATCTTGCGCTCGTAAAGGCTCAGTTGCGCAAGCAAGGTATCAACTTCACCAAGGTACGTAAAAAGCCCG
TTTCGATCTTTGGCAAGGGCAAGAAGATCAAGCCGCTGGACATCGCATTCTTTTCTCGGCAGATGGCGACCATGATGAAG
GCAGGTGTGCCGCTTCTGCAGTCATTTGACATCATTGCCGAGGGTGCGGAAAACCCGAACATGCGTGCACTGGTTGGCTC
ACTGAAGCAGGAGGTGTCGGCAGGGAACAGCTTTGCGACGGCTCTGCGACAGAAACCGGAATACTTCGATGACCTGTTTT
GTAATTTGGTCGATGCGGGCGAGCAAGCAGGTGCACTCGAGAGTCTCTTGGACCGCGTCGCTAGCTACAAGGAAAAGACT
GAAAAGCTCAAAGCAAAAATCAAAAAAGCCATGACATACCCTATTGCTGTGTTGATCGTCGCAATTATCGTGTCAGGTAT
TTTGCTCATCAAAGTGGTGCCGCAGTTTCAGTCAGTTTTTGCAGGCTTTGGCGCAGAGCTACCCGCATTTACTCTCATGG
TAATCGGCTTATCAGATATCGTACAGAAATGGTGGTTAGCAATAGTGGGTCTTTTTTTCGTAGGCGCTTTTTTGTTCAAA
CGAGCTTATAAGCAGTCTGAAAAATTTCGCGATAATATTGATCGGTTTCTGCTGAAAGTACCGATCATTGGACCGCTTAT
TTTCAAGTCTTCCGTAGCTCGCTACGCGCGGACGCTTGCAACAACATTCGCAGCAGGCGTTCCTCTAGTGGAGGCACTTG
ACTCAGTTGCAGGTGCTACCGGCAATGTTGTTTTCAGAGACGCTGTAAACAAGGTCAAACAAGACGTTTCCACAGGCATG
CAGTTGAATTTTTCGATGCGATCGACCGGTGTTTTTCCAAGCTTGGCAATCCAGATGACGGCCATAGGCGAGGAATCAGG
CGCGTTGGACAATATGCTGGACAAGGTAGCGACCTACTACGAGGATGAGGTCGACAATATGGTCGACAACTTAACCAGCC
TGATGGAGCCCATGATCATGGCTGTGCTGGGTGTCATAGTCGGCGGCCTTGTTATCGCCATGTACCTCCCCATTTTCAAA
CTCGGAAACGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.815

100

0.748
  pilC Acinetobacter baylyi ADP1

59.756

100

0.605
  pilC Acinetobacter baumannii D1279779

59.158

99.753

0.59
  pilC Legionella pneumophila strain ERS1305867

53.03

97.778

0.519
  pilG Neisseria gonorrhoeae MS11

43.672

99.506

0.435
  pilG Neisseria meningitidis 44/76-A

43.672

99.506

0.435
  pilC Vibrio campbellii strain DS40M4

42.015

100

0.422
  pilC Vibrio cholerae strain A1552

41.728

100

0.417
  pilC Thermus thermophilus HB27

38.557

99.259

0.383

Multiple sequence alignment