Detailed information NTG0221581

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: B5U27_RS04565
• Genome accession: NZ_CP020351
• Coordinates: 963371..964243 (-)
• Length: 290 a.a.
• NCBI ID: WP_005745479.1
• Uniprot ID: A0AB37R9U1
• Organism: Pseudomonas amygdali pv. lachrymans strain NM002
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 958371..969243

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B5U27_RS04525 (B5U27_04525)	-	959270..960568 (-)	1299	WP_005745472.1	NAD(P)/FAD-dependent oxidoreductase	-
B5U27_RS04530 (B5U27_04530)	-	960665..960772 (-)	108	WP_080164415.1	DUF3094 family protein	-
B5U27_RS04535 (B5U27_04535)	-	960786..961319 (-)	534	WP_005745473.1	MOSC domain-containing protein	-
B5U27_RS04540 (B5U27_04540)	-	961316..961945 (-)	630	WP_003430735.1	DUF1780 domain-containing protein	-
B5U27_RS04545 (B5U27_04545)	-	962060..962344 (+)	285	Protein_851	hypothetical protein	-
B5U27_RS04555 (B5U27_04555)	yacG	962545..962754 (-)	210	WP_005745477.1	DNA gyrase inhibitor YacG	-
B5U27_RS04560 (B5U27_04560)	coaE	962751..963374 (-)	624	WP_005745478.1	dephospho-CoA kinase	-
B5U27_RS04565 (B5U27_04565)	pilD	963371..964243 (-)	873	WP_005745479.1	prepilin peptidase	Machinery gene
B5U27_RS04570 (B5U27_04570)	pilC	964245..965462 (-)	1218	WP_005745480.1	type II secretion system F family protein	Machinery gene
B5U27_RS04575 (B5U27_04575)	pilB	965465..967159 (-)	1695	WP_005745482.1	type IV-A pilus assembly ATPase PilB	Machinery gene
B5U27_RS04580 (B5U27_04580)	-	967385..967771 (+)	387	WP_005745483.1	pilin	-
B5U27_RS04585 (B5U27_04585)	-	968117..969094 (+)	978	WP_007247761.1	IS5 family transposase	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32049.32 Da
• Isoelectric Point: 8.5809

>NTDB_id=221581 B5U27_RS04565 WP_005745479.1 963371..964243(-) (pilD) [Pseudomonas amygdali pv. lachrymans strain NM002]
MPLLDLLASSPLAFVITCCILGMIIGSFLNVVVYRLPKMMERDWKAQSREMLGLPAEPDQPAFNLNRPRSSCPHCAHKIR
PRENLPLISYLLLRGKCSQCKAPISMRYPMVELTCAVLSAYVAWHFGFGWQAAAMLVLSWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=221581 B5U27_RS04565 WP_005745479.1 963371..964243(-) (pilD) [Pseudomonas amygdali pv. lachrymans strain NM002]
ATGCCCCTCCTCGACCTCCTGGCCAGCTCACCGCTGGCCTTCGTCATTACCTGCTGCATCCTCGGCATGATCATCGGAAG
CTTCCTCAACGTAGTCGTCTACCGCCTCCCCAAAATGATGGAGCGCGACTGGAAAGCCCAATCCCGTGAAATGCTCGGCC
TGCCCGCCGAACCTGATCAGCCTGCCTTCAACCTGAACCGCCCCCGCTCCAGTTGCCCGCACTGCGCGCACAAGATCCGC
CCGAGGGAAAACCTGCCGCTGATCAGCTATCTGCTGCTGCGCGGTAAATGCTCACAATGCAAAGCCCCCATCAGCATGCG
CTACCCAATGGTCGAGCTGACCTGCGCAGTGTTGTCGGCCTACGTCGCCTGGCATTTCGGCTTCGGCTGGCAGGCAGCGG
CGATGCTGGTGTTGAGCTGGGGCCTGCTGGCGATGAGTCTGATCGACGCCGACCACCAGCTGTTACCTGACTCTCTGGTG
CTGCCACTGCTGTGGCTGGGCCTCATCGTCAACGCTTTCGGGCTGTTCACCTCGCTGAACGATGCGCTGTGGGGCGCGGT
GGCCGGCTATCTGGCGCTGTGGTCGGTGTTCTGGCTATTTAAACTGGTGACCGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTGGCCATGCTTGGCGCATGGGGCGGCTGGCAGATTCTGCCGTTGACCATCCTGCTGTCATCGCTGGTGGGT
GCGGTGCTTGGGGTGATCATGATGCGCGTGCGCAGGGTTGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (136-245)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.169	97.931	0.521
pilD	Vibrio campbellii strain DS40M4	51.943	97.586	0.507
pilD	Acinetobacter baumannii D1279779	52.92	94.483	0.5
pilD	Acinetobacter nosocomialis M2	52.555	94.483	0.497
pilD	Neisseria gonorrhoeae MS11	50.373	92.414	0.466