Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   B5M06_RS08045 Genome accession   NZ_CP020121
Coordinates   1719788..1721011 (-) Length   407 a.a.
NCBI ID   WP_054067379.1    Uniprot ID   A0A0W7YZY7
Organism   Comamonas kerstersii strain 8943     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1714788..1726011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5M06_RS08015 (B5M06_08015) - 1715744..1716676 (+) 933 WP_054067373.1 ATP-binding protein -
  B5M06_RS08020 (B5M06_08020) - 1716695..1717153 (+) 459 WP_420809977.1 NUDIX domain-containing protein -
  B5M06_RS08025 (B5M06_08025) - 1717239..1717442 (-) 204 WP_054067375.1 DNA gyrase inhibitor YacG -
  B5M06_RS08030 (B5M06_08030) zapD 1717470..1718225 (-) 756 WP_054067376.1 cell division protein ZapD -
  B5M06_RS08035 (B5M06_08035) coaE 1718308..1718916 (-) 609 WP_054067377.1 dephospho-CoA kinase -
  B5M06_RS08040 (B5M06_08040) - 1718919..1719788 (-) 870 WP_054067378.1 prepilin peptidase -
  B5M06_RS08045 (B5M06_08045) pilC 1719788..1721011 (-) 1224 WP_054067379.1 type II secretion system F family protein Machinery gene
  B5M06_RS08050 (B5M06_08050) pilB 1721093..1722835 (-) 1743 WP_054067380.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B5M06_RS08055 (B5M06_08055) - 1722985..1723914 (-) 930 WP_054067381.1 polyprenyl synthetase family protein -
  B5M06_RS08060 (B5M06_08060) rplU 1724126..1724437 (+) 312 WP_054067382.1 50S ribosomal protein L21 -
  B5M06_RS08065 (B5M06_08065) rpmA 1724456..1724716 (+) 261 WP_054067383.1 50S ribosomal protein L27 -
  B5M06_RS08070 (B5M06_08070) cgtA 1724876..1725949 (+) 1074 WP_054067384.1 Obg family GTPase CgtA -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 44734.82 Da        Isoelectric Point: 9.3792

>NTDB_id=221366 B5M06_RS08045 WP_054067379.1 1719788..1721011(-) (pilC) [Comamonas kerstersii strain 8943]
MANATIGRAPAKEFIFEWVGKDRQGKELRGEMRATGEAQVQASLRRQGIFMTKLKKRALRAGKKIKPKDIAVFTRQMATM
MKAGVPLLQAFDIVGRGNPNPSVTKLLNDIRQDVETGTSLSVAFRKHPMYFDALYCNLVEAGEAAGILETLMDRLATYME
KTEAIKGKIKSALMYPTSVVLVAFIVVTVIMIFVIPAFKEVFTSFGADLPAPTLLVMSISEFFVDYWWLIFGVLGGGFYF
FMQAWKRSPKMQRVMDRLLLKMPIFGVLIDKSCVARWTRTLSTMFAAGVPLVEALDSVGGASGNSVYKEATDRIQHEVAT
GSSLNVAMANANVFPSMVLQMCAIGEESGAIDHMLGKAADFYEQEVDEMVAGLSSLMEPIIIVFLGGIIGSIVVAMYLPI
FKLGQVV

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=221366 B5M06_RS08045 WP_054067379.1 1719788..1721011(-) (pilC) [Comamonas kerstersii strain 8943]
ATGGCAAACGCAACAATTGGCCGAGCGCCAGCCAAGGAATTCATCTTTGAATGGGTAGGCAAAGACCGGCAGGGCAAAGA
GCTGCGCGGCGAAATGCGTGCCACAGGAGAAGCACAGGTCCAGGCGTCGCTGCGCCGCCAGGGCATCTTCATGACCAAGC
TGAAAAAGCGCGCCCTGCGGGCTGGCAAGAAGATCAAGCCCAAGGACATTGCCGTCTTCACGCGCCAGATGGCCACCATG
ATGAAGGCGGGCGTGCCCTTGCTGCAGGCCTTCGACATCGTGGGCCGCGGCAACCCCAACCCCAGCGTGACCAAGCTGCT
CAACGACATCCGTCAGGATGTAGAGACCGGCACTTCACTGAGTGTGGCGTTTCGCAAGCACCCCATGTACTTCGATGCGC
TGTACTGCAACCTGGTCGAAGCGGGTGAAGCCGCAGGTATTCTGGAAACCCTGATGGATCGCCTGGCCACCTATATGGAA
AAGACCGAGGCCATCAAGGGCAAGATCAAATCAGCCCTGATGTACCCCACCTCGGTCGTGCTGGTGGCCTTTATTGTGGT
GACGGTGATCATGATCTTTGTGATCCCTGCCTTCAAGGAAGTCTTTACCTCCTTTGGTGCCGATCTGCCCGCCCCCACCC
TGCTGGTGATGTCCATCAGCGAGTTCTTTGTGGACTACTGGTGGCTGATCTTTGGCGTGCTCGGTGGAGGTTTTTACTTT
TTCATGCAGGCCTGGAAGCGCAGCCCCAAGATGCAGCGCGTCATGGACCGCCTGCTGCTGAAAATGCCCATCTTTGGCGT
GCTGATCGACAAATCCTGCGTGGCGCGCTGGACGCGTACGCTCTCGACCATGTTTGCCGCAGGCGTGCCCCTGGTGGAAG
CACTGGACTCTGTGGGCGGCGCTTCCGGCAACAGCGTCTACAAGGAGGCCACCGACCGCATCCAGCATGAAGTAGCCACC
GGCTCCAGCCTGAACGTGGCCATGGCCAATGCCAATGTCTTTCCATCCATGGTGCTGCAGATGTGCGCCATTGGCGAGGA
GTCGGGTGCCATTGACCACATGCTGGGCAAGGCTGCCGACTTCTACGAACAAGAGGTCGATGAGATGGTGGCCGGGCTTT
CCAGCCTGATGGAGCCCATCATCATCGTGTTTCTGGGTGGCATCATCGGCAGCATCGTGGTGGCCATGTACCTGCCTATC
TTCAAATTGGGACAAGTGGTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(73-196)

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0W7YZY7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

55.138

98.034

0.541
  pilG Neisseria gonorrhoeae MS11

53.149

97.543

0.518
  pilG Neisseria meningitidis 44/76-A

52.897

97.543

0.516
  pilC Legionella pneumophila strain ERS1305867

50.758

97.297

0.494
  pilC Acinetobacter baylyi ADP1

49.497

97.789

0.484
  pilC Acinetobacter baumannii D1279779

47.859

97.543

0.467
  pilC Vibrio cholerae strain A1552

41.414

97.297

0.403
  pilC Vibrio campbellii strain DS40M4

40.5

98.28

0.398
  pilC Thermus thermophilus HB27

39.098

98.034

0.383

Multiple sequence alignment