Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   B5M06_RS08050 Genome accession   NZ_CP020121
Coordinates   1721093..1722835 (-) Length   580 a.a.
NCBI ID   WP_054067380.1    Uniprot ID   A0A0W7Z0I8
Organism   Comamonas kerstersii strain 8943     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1716093..1727835
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5M06_RS08020 (B5M06_08020) - 1716695..1717153 (+) 459 WP_420809977.1 NUDIX domain-containing protein -
  B5M06_RS08025 (B5M06_08025) - 1717239..1717442 (-) 204 WP_054067375.1 DNA gyrase inhibitor YacG -
  B5M06_RS08030 (B5M06_08030) zapD 1717470..1718225 (-) 756 WP_054067376.1 cell division protein ZapD -
  B5M06_RS08035 (B5M06_08035) coaE 1718308..1718916 (-) 609 WP_054067377.1 dephospho-CoA kinase -
  B5M06_RS08040 (B5M06_08040) - 1718919..1719788 (-) 870 WP_054067378.1 prepilin peptidase -
  B5M06_RS08045 (B5M06_08045) pilC 1719788..1721011 (-) 1224 WP_054067379.1 type II secretion system F family protein Machinery gene
  B5M06_RS08050 (B5M06_08050) pilB 1721093..1722835 (-) 1743 WP_054067380.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B5M06_RS08055 (B5M06_08055) - 1722985..1723914 (-) 930 WP_054067381.1 polyprenyl synthetase family protein -
  B5M06_RS08060 (B5M06_08060) rplU 1724126..1724437 (+) 312 WP_054067382.1 50S ribosomal protein L21 -
  B5M06_RS08065 (B5M06_08065) rpmA 1724456..1724716 (+) 261 WP_054067383.1 50S ribosomal protein L27 -
  B5M06_RS08070 (B5M06_08070) cgtA 1724876..1725949 (+) 1074 WP_054067384.1 Obg family GTPase CgtA -
  B5M06_RS08075 (B5M06_08075) proB 1726031..1727167 (+) 1137 WP_054067385.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 63166.56 Da        Isoelectric Point: 6.2054

>NTDB_id=221367 B5M06_RS08050 WP_054067380.1 1721093..1722835(-) (pilB) [Comamonas kerstersii strain 8943]
MTAVTNAPSLAPTGTMQLPGLARALVSAGQLSEATAREVALKATASKSSFVAQLVSSGHFSAAELAHQISLIFSTPLLDL
NAIDPLLLPKDLLDPKLCTSHHVLPLCKRGNRLIVATAAPTDKEATEKIKFASQMGIDWIIAEHDKLVRLIESAHKSVTE
ALDTYIADDEFQFDDTKEAPQQEDKDSSASDVEDAPVVKFLHKMLIDAVNMRASDLHFEPYEHNYRVRFRVDGELREIAS
PPITIKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPERVIDFRVSTLPTLFGEKIVIRILDPNSAKMGIDALGYEPEE
KARLLDAIHRPYGMILVTGPTGSGKTVSLYTCLNILNQPGVNISTAEDPSEINMPGVNQVNVNDKAGLTFAAALKAFLRQ
DPDVIMVGEIRDLETADIAIKAAQTGHMVLSTLHTNDAPTTLTRLRNMGVAPFNIASSVILITAQRLARRLCPQCKQPHD
VPTEALLAAGFEESQLDGSWVPYKAVGCSACNNGYKGRVGIYQVMPISEEIQQIILRDGSALDIANQAKREGVKSLRESG
LDKVRQGVTSLEEVLAVTNL

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=221367 B5M06_RS08050 WP_054067380.1 1721093..1722835(-) (pilB) [Comamonas kerstersii strain 8943]
ATGACTGCTGTTACCAACGCCCCATCACTTGCACCGACTGGCACCATGCAGCTTCCGGGCTTAGCCCGTGCTTTAGTCAG
TGCTGGGCAGCTATCAGAAGCTACTGCTCGTGAAGTCGCCCTCAAGGCCACTGCCAGCAAAAGCAGCTTTGTAGCGCAGC
TGGTCAGCAGCGGCCATTTCTCTGCCGCAGAACTAGCCCACCAGATCTCGCTGATCTTCAGCACGCCACTGCTGGATCTG
AACGCCATTGACCCGCTGCTGCTGCCCAAGGATCTGCTCGACCCCAAGCTGTGCACCAGCCACCATGTGCTGCCGCTGTG
CAAGCGCGGCAACCGCCTGATCGTGGCCACGGCGGCCCCCACCGACAAGGAAGCCACGGAAAAAATCAAATTTGCCTCTC
AGATGGGCATTGACTGGATCATTGCCGAGCACGACAAACTGGTCAGACTCATTGAGAGCGCACACAAAAGCGTCACGGAA
GCGCTGGACACTTATATTGCAGACGACGAATTCCAGTTCGACGATACCAAGGAAGCTCCCCAGCAGGAGGACAAGGACAG
CAGCGCTTCGGACGTAGAAGATGCTCCCGTTGTCAAATTCCTGCACAAGATGCTGATCGATGCCGTCAACATGCGCGCAT
CGGACCTGCACTTTGAGCCGTATGAGCACAACTACCGCGTGCGCTTTCGTGTGGACGGTGAGCTGCGTGAAATTGCCTCG
CCACCTATCACCATCAAGGACAAGCTGGCCTCGCGCATCAAGGTGATTTCACGTCTGGACATTTCGGAAAAACGCGTGCC
ACAGGATGGCCGCATGAAGCTCAAGGTCGGCCCTGAACGGGTGATTGACTTCCGTGTGTCCACCCTGCCCACGCTGTTTG
GCGAAAAAATCGTCATCCGTATCTTGGACCCCAACAGCGCCAAGATGGGCATTGATGCCCTGGGCTATGAGCCCGAAGAA
AAAGCACGCCTGCTCGATGCCATCCATCGTCCCTACGGCATGATTCTGGTGACCGGCCCCACCGGCTCGGGCAAGACGGT
GTCGCTCTACACCTGCCTGAACATCCTCAATCAGCCAGGCGTCAACATCTCGACCGCTGAAGACCCTTCCGAAATCAACA
TGCCGGGGGTCAACCAGGTCAACGTCAATGACAAGGCGGGCCTGACCTTTGCTGCTGCGCTCAAGGCGTTTTTGCGTCAG
GACCCGGACGTGATCATGGTCGGTGAAATCCGTGACCTGGAAACTGCAGACATCGCCATCAAGGCCGCACAGACCGGCCA
CATGGTGCTCTCGACCCTGCACACCAACGATGCCCCCACCACGCTGACGCGTCTGCGCAACATGGGGGTAGCACCGTTCA
ACATCGCCTCCAGCGTCATCCTGATTACGGCCCAGCGTCTGGCACGTCGCCTGTGCCCCCAATGCAAGCAGCCGCACGAT
GTCCCGACGGAAGCGCTGCTGGCCGCAGGCTTTGAAGAAAGCCAGCTCGACGGCTCCTGGGTGCCGTACAAGGCCGTAGG
CTGCAGCGCCTGCAACAATGGCTACAAGGGCCGCGTCGGCATCTACCAGGTCATGCCCATCAGCGAAGAAATACAGCAAA
TCATCCTGCGTGATGGCTCCGCACTGGACATTGCCAACCAGGCCAAGCGCGAAGGCGTCAAATCCTTGCGTGAATCAGGT
TTGGACAAGGTCCGGCAAGGCGTTACCTCGCTGGAAGAAGTTCTGGCAGTTACCAACCTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-154)

Type II/IV secretion system protein (T2SSE)

(203-470)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0W7Z0I8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.782

100

0.566
  pilB Acinetobacter baumannii D1279779

56.383

97.241

0.548
  pilF Neisseria gonorrhoeae MS11

53.641

97.069

0.521
  pilB Legionella pneumophila strain ERS1305867

50

98.966

0.495
  pilB Vibrio cholerae strain A1552

48.294

96.034

0.464
  pilB Vibrio campbellii strain DS40M4

46.099

97.241

0.448
  pilB Vibrio parahaemolyticus RIMD 2210633

45.518

98.103

0.447
  pilF Thermus thermophilus HB27

40.672

92.414

0.376
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.112

92.414

0.371

Multiple sequence alignment