Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   B4X03_RS09685 Genome accession   NZ_CP020085
Coordinates   2032047..2033432 (+) Length   461 a.a.
NCBI ID   WP_010787006.1    Uniprot ID   -
Organism   Glaesserella parasuis strain CL120103     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2027047..2038432
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B4X03_RS09660 - 2027053..2028507 (-) 1455 WP_082259364.1 aminoacyl-histidine dipeptidase -
  B4X03_RS09665 gpt 2028687..2029166 (+) 480 WP_021114522.1 xanthine phosphoribosyltransferase -
  B4X03_RS09670 gmk 2029292..2029918 (+) 627 WP_021113158.1 guanylate kinase -
  B4X03_RS09675 radA 2029971..2031347 (-) 1377 WP_021114521.1 DNA repair protein RadA -
  B4X03_RS09680 pilA 2031535..2031999 (+) 465 WP_005711781.1 pilin Machinery gene
  B4X03_RS09685 pilB 2032047..2033432 (+) 1386 WP_010787006.1 GspE/PulE family protein Machinery gene
  B4X03_RS09690 pilC 2033425..2034621 (+) 1197 WP_005711777.1 type II secretion system F family protein Machinery gene
  B4X03_RS09695 - 2034618..2035286 (+) 669 WP_043896635.1 prepilin peptidase -
  B4X03_RS09700 dapE 2035370..2036503 (+) 1134 WP_021114519.1 succinyl-diaminopimelate desuccinylase -
  B4X03_RS09705 clpX 2036577..2037827 (-) 1251 WP_021114518.1 ATP-dependent protease ATP-binding subunit ClpX -
  B4X03_RS09710 clpP 2037834..2038415 (-) 582 WP_005711766.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52808.65 Da        Isoelectric Point: 5.3881

>NTDB_id=220974 B4X03_RS09685 WP_010787006.1 2032047..2033432(+) (pilB) [Glaesserella parasuis strain CL120103]
MSQYTICEQQTERIFDISPSRWQKNCEEKQLLLRYLAVPVQETEHKLWLAVDDENNLTACEIFAFMTHKQIEPVVIASDE
LKYLLNALSPEQQPIYEESELAFVEQEQEQLNLNDPIIQLLDNLFKFCLAQNASDIHIEPRKQKLIIRLRIDGVLHLYKS
LSIQLASRLISRIKLLAKLDISELRQPQDGQFSFTTALADTLDFRVSSLPTIYGEKLVLRLQKNKPTSFDFLQLGFNPQQ
QTTLLNALKQPQGLILVTGPTGSGKSITLYSALNYLNQMDKHILTAEDPVEIEIDGLIQTQVNRSIQLDFSQLLRTFLRQ
DPDIIMLGEIRDEESAKMALRASQTGHLVLSTLHTNDALSAVERLLQLGIQEYEIQNSLLLVIAQRLVRKLCKKCRGVGC
DECYQGYRGRIGVYQCLSRTAKKFDKQTAYLDFSSLRESAKQKIEQQLTNETEVDRVLGYE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=220974 B4X03_RS09685 WP_010787006.1 2032047..2033432(+) (pilB) [Glaesserella parasuis strain CL120103]
ATGTCCCAATACACTATCTGCGAACAACAAACCGAACGCATTTTTGATATATCGCCTAGCCGTTGGCAAAAAAATTGCGA
GGAGAAACAGCTTTTATTGCGTTACCTTGCCGTTCCTGTGCAAGAAACTGAGCATAAATTATGGCTTGCCGTTGATGATG
AAAACAATCTCACCGCTTGCGAAATCTTTGCTTTTATGACCCATAAGCAGATTGAGCCGGTGGTGATTGCTTCTGATGAA
CTGAAATACTTGCTTAACGCTCTCTCGCCTGAACAACAGCCCATTTATGAAGAGAGCGAGCTGGCTTTTGTTGAACAGGA
GCAAGAACAGCTCAACCTAAACGATCCGATTATTCAACTGCTAGATAATCTCTTTAAATTTTGTCTAGCTCAGAATGCGT
CCGACATTCATATTGAGCCACGCAAGCAAAAATTGATTATCCGCTTACGCATTGACGGCGTTTTGCACCTGTATAAAAGC
CTATCTATTCAGCTGGCAAGCCGACTAATTTCACGGATAAAACTACTGGCAAAGCTAGATATTAGCGAGTTACGCCAGCC
ACAAGATGGGCAATTTAGTTTTACCACCGCCCTTGCGGATACCCTTGATTTTCGGGTGTCTAGCCTGCCGACGATCTACG
GTGAAAAACTGGTATTACGCCTACAAAAAAACAAGCCGACCAGTTTTGATTTTCTACAACTTGGCTTTAATCCACAACAG
CAAACCACACTGCTCAACGCCCTGAAACAACCACAGGGGCTGATTTTAGTGACCGGACCAACGGGCAGTGGCAAAAGCAT
CACCCTGTACAGTGCCTTAAACTATCTTAACCAAATGGATAAACATATTCTCACCGCCGAAGATCCGGTGGAAATCGAGA
TTGACGGCTTAATTCAAACCCAAGTCAATCGCAGTATTCAACTGGATTTTAGCCAGCTGTTACGCACCTTTCTACGTCAA
GATCCCGACATCATTATGTTAGGCGAAATTCGAGATGAAGAGAGTGCCAAAATGGCGTTACGAGCAAGCCAGACGGGGCA
TTTAGTCCTTTCGACCCTGCATACCAACGACGCACTCTCTGCGGTCGAACGCTTGTTGCAACTGGGTATTCAAGAGTATG
AAATCCAAAATTCACTACTGTTAGTGATTGCACAACGCCTTGTGCGTAAGCTCTGTAAAAAGTGTCGTGGAGTAGGCTGT
GACGAGTGCTATCAAGGCTATAGAGGGCGGATCGGGGTTTACCAATGTTTAAGCAGAACGGCAAAAAAATTCGATAAACA
GACCGCTTACTTGGATTTTTCCAGCCTACGAGAGAGTGCTAAACAGAAAATTGAACAACAACTCACCAATGAAACCGAAG
TGGATAGGGTATTAGGTTATGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(30-92)

Type II/IV secretion system protein (T2SSE)

(129-390)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

100

100

1
  pilB Haemophilus influenzae Rd KW20

58.093

97.831

0.568
  pilB Haemophilus influenzae 86-028NP

57.456

98.915

0.568
  pilB Vibrio cholerae strain A1552

38.2

100

0.414
  pilB Vibrio parahaemolyticus RIMD 2210633

39.13

100

0.41
  pilB Legionella pneumophila strain ERS1305867

38.211

100

0.408
  pilB Vibrio campbellii strain DS40M4

38.923

100

0.408
  pilB Acinetobacter baumannii D1279779

36.187

100

0.403
  pilB Acinetobacter baylyi ADP1

36.634

100

0.401
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.248

100

0.388
  pilF Neisseria gonorrhoeae MS11

36.421

100

0.375
  pilF Thermus thermophilus HB27

35.01

100

0.362

Multiple sequence alignment