Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   B4X03_RS09680 Genome accession   NZ_CP020085
Coordinates   2031535..2031999 (+) Length   154 a.a.
NCBI ID   WP_005711781.1    Uniprot ID   K4JWE9
Organism   Glaesserella parasuis strain CL120103     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2026535..2036999
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B4X03_RS09660 - 2027053..2028507 (-) 1455 WP_082259364.1 aminoacyl-histidine dipeptidase -
  B4X03_RS09665 gpt 2028687..2029166 (+) 480 WP_021114522.1 xanthine phosphoribosyltransferase -
  B4X03_RS09670 gmk 2029292..2029918 (+) 627 WP_021113158.1 guanylate kinase -
  B4X03_RS09675 radA 2029971..2031347 (-) 1377 WP_021114521.1 DNA repair protein RadA -
  B4X03_RS09680 pilA 2031535..2031999 (+) 465 WP_005711781.1 pilin Machinery gene
  B4X03_RS09685 pilB 2032047..2033432 (+) 1386 WP_010787006.1 GspE/PulE family protein Machinery gene
  B4X03_RS09690 pilC 2033425..2034621 (+) 1197 WP_005711777.1 type II secretion system F family protein Machinery gene
  B4X03_RS09695 - 2034618..2035286 (+) 669 WP_043896635.1 prepilin peptidase -
  B4X03_RS09700 dapE 2035370..2036503 (+) 1134 WP_021114519.1 succinyl-diaminopimelate desuccinylase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16379.04 Da        Isoelectric Point: 9.3428

>NTDB_id=220973 B4X03_RS09680 WP_005711781.1 2031535..2031999(+) (pilA) [Glaesserella parasuis strain CL120103]
MKKIRLIRPLAKAFTLIELMIVIAIIAILATIAIPSYTSYTQKAALSELLSASASYKSDVEICIYNVGDKKISECSSGKN
GVRADKSDVPDAKYLKSISVASGVITVAGKGNIDGYGYTMTPKFANNNITWATSCQGADISLFPANFCGQKATP

Nucleotide


Download         Length: 465 bp        

>NTDB_id=220973 B4X03_RS09680 WP_005711781.1 2031535..2031999(+) (pilA) [Glaesserella parasuis strain CL120103]
ATGAAAAAAATCAGGTTAATTCGACCGCTTGCAAAAGCCTTTACCTTAATTGAGTTAATGATCGTGATCGCCATCATTGC
CATTTTAGCAACAATAGCAATCCCGTCTTACACCAGTTACACCCAAAAGGCAGCTCTCTCTGAGTTATTAAGTGCTTCCG
CATCTTATAAATCCGATGTGGAAATTTGTATTTATAACGTAGGGGATAAAAAAATCTCGGAATGTAGTTCAGGTAAAAAT
GGCGTTCGAGCAGATAAATCCGATGTCCCTGACGCAAAATATCTTAAATCTATTTCCGTCGCTTCGGGGGTAATCACCGT
GGCAGGTAAAGGCAATATTGATGGCTACGGCTACACAATGACCCCGAAATTTGCCAATAACAACATCACTTGGGCAACAA
GCTGTCAAGGGGCTGACATTAGTTTGTTCCCTGCGAATTTTTGTGGGCAGAAAGCTACACCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(12-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K4JWE9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

100

100

1
  pilA Haemophilus influenzae Rd KW20

55.034

96.753

0.532
  pilA Haemophilus influenzae 86-028NP

54.362

96.753

0.526

Multiple sequence alignment