Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   B5D85_RS00655 Genome accession   NZ_CP020082
Coordinates   102264..103202 (+) Length   312 a.a.
NCBI ID   WP_002986557.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain STAB120304     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 97264..108202
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5D85_RS00645 (B5D85_00645) rpoC 98013..101654 (+) 3642 WP_020904830.1 DNA-directed RNA polymerase subunit beta' -
  B5D85_RS00650 (B5D85_00650) - 101806..102171 (+) 366 WP_002986560.1 DUF1033 family protein -
  B5D85_RS00655 (B5D85_00655) comYA 102264..103202 (+) 939 WP_002986557.1 competence type IV pilus ATPase ComGA Machinery gene
  B5D85_RS00660 (B5D85_00660) comYB 103138..104172 (+) 1035 WP_228631569.1 competence type IV pilus assembly protein ComGB Machinery gene
  B5D85_RS00665 (B5D85_00665) comYC 104174..104500 (+) 327 WP_011528161.1 competence type IV pilus major pilin ComGC Machinery gene
  B5D85_RS00670 (B5D85_00670) comGD 104475..104903 (+) 429 WP_076639399.1 competence type IV pilus minor pilin ComGD -
  B5D85_RS00675 (B5D85_00675) comGE 104860..105144 (+) 285 WP_307777288.1 competence type IV pilus minor pilin ComGE -
  B5D85_RS00680 (B5D85_00680) comYF 105137..105571 (+) 435 WP_002986542.1 competence type IV pilus minor pilin ComGF Machinery gene
  B5D85_RS00685 (B5D85_00685) comGG 105555..105881 (+) 327 WP_002986539.1 competence type IV pilus minor pilin ComGG -
  B5D85_RS00690 (B5D85_00690) comYH 105979..106932 (+) 954 WP_010921802.1 class I SAM-dependent methyltransferase Machinery gene
  B5D85_RS00695 (B5D85_00695) - 106991..108187 (+) 1197 WP_020904834.1 acetate kinase -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35087.35 Da        Isoelectric Point: 8.5799

>NTDB_id=220889 B5D85_RS00655 WP_002986557.1 102264..103202(+) (comYA) [Streptococcus pyogenes strain STAB120304]
MVQVLAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVDVYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYK
LSKDKQLSLRLSSVGDYRGQESLVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISNYHQEA
QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAVIRASLTGAMVFSTVHAKSISGV
YARLLELGVTKAELSNCLALIAYQRLLNGGALIDSTQNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII

Nucleotide


Download         Length: 939 bp        

>NTDB_id=220889 B5D85_RS00655 WP_002986557.1 102264..103202(+) (comYA) [Streptococcus pyogenes strain STAB120304]
ATGGTACAAGTATTAGCAAAAGCTATTCTAGCAAAAGCTGAACAGGTTCAGGCACAAGATATTTATATTTTGCCAAGAGC
AGATCAATATGATCTTTTTTTACGAATAGGAGATGAAAGGAGATTAGTTGATGTTTATCAGAGCGATCGGATGGCTCCTC
TTATTAGTCACTTTAAATTCGTTGCAGGAATGATAGTTGGTGAAAAGAGACGTTGTCAGGTGGGTTCATGTGATTATAAG
CTTAGTAAAGATAAGCAGTTATCTTTGCGCTTATCTAGCGTGGGTGATTATCGCGGGCAAGAAAGCTTAGTGATTCGTCT
GCTTCATCATCAAAATAAAAGTGTACATTATTGGTTTGATGGATTGACAAAAGTAGCCAATCAGGTTGGCGGTAGAGGGT
TGTATTTATTTGCAGGACCAGTTGGGTCTGGGAAGACAACCTTGATGTACCAGCTGATTTCGAATTATCATCAAGAAGCA
CAGGTTATTAGTATAGAAGATCCTGTAGAAATTAAAAATCACCAGATTTTACAATTACAAGTGAATGATGATATTGGTAT
GACTTATGACAATTTGATCAAACTGTCTTTACGCCATCGACCAGATATTTTAGTTATTGGTGAGATTCGAGATAGTCAAA
CAGCAAGAGCCGTTATTAGGGCTAGTCTAACAGGTGCCATGGTTTTTTCAACGGTTCACGCTAAAAGTATCTCGGGTGTT
TATGCAAGATTGTTAGAACTTGGTGTAACGAAAGCAGAACTGTCTAATTGCTTAGCATTAATTGCTTACCAAAGGTTACT
TAATGGAGGAGCATTGATTGACTCTACTCAAAACGAATTTGAATATTATTCCTCATCGAACTGGAATCAACAAATTGATC
AGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

61.736

99.679

0.615
  comYA Streptococcus mutans UA140

61.736

99.679

0.615
  comYA Streptococcus gordonii str. Challis substr. CH1

60.772

99.679

0.606
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.645

99.359

0.603
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

59.486

99.679

0.593
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae D39

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae R6

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

58.842

99.679

0.587
  comGA Lactococcus lactis subsp. cremoris KW2

50.482

99.679

0.503
  comGA Latilactobacillus sakei subsp. sakei 23K

39.655

92.949

0.369

Multiple sequence alignment