Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   B5D85_RS00680 Genome accession   NZ_CP020082
Coordinates   105137..105571 (+) Length   144 a.a.
NCBI ID   WP_002986542.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain STAB120304     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 100137..110571
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5D85_RS00650 (B5D85_00650) - 101806..102171 (+) 366 WP_002986560.1 DUF1033 family protein -
  B5D85_RS00655 (B5D85_00655) comYA 102264..103202 (+) 939 WP_002986557.1 competence type IV pilus ATPase ComGA Machinery gene
  B5D85_RS00660 (B5D85_00660) comYB 103138..104172 (+) 1035 WP_228631569.1 competence type IV pilus assembly protein ComGB Machinery gene
  B5D85_RS00665 (B5D85_00665) comYC 104174..104500 (+) 327 WP_011528161.1 competence type IV pilus major pilin ComGC Machinery gene
  B5D85_RS00670 (B5D85_00670) comGD 104475..104903 (+) 429 WP_076639399.1 competence type IV pilus minor pilin ComGD -
  B5D85_RS00675 (B5D85_00675) comGE 104860..105144 (+) 285 WP_307777288.1 competence type IV pilus minor pilin ComGE -
  B5D85_RS00680 (B5D85_00680) comYF 105137..105571 (+) 435 WP_002986542.1 competence type IV pilus minor pilin ComGF Machinery gene
  B5D85_RS00685 (B5D85_00685) comGG 105555..105881 (+) 327 WP_002986539.1 competence type IV pilus minor pilin ComGG -
  B5D85_RS00690 (B5D85_00690) comYH 105979..106932 (+) 954 WP_010921802.1 class I SAM-dependent methyltransferase Machinery gene
  B5D85_RS00695 (B5D85_00695) - 106991..108187 (+) 1197 WP_020904834.1 acetate kinase -
  B5D85_RS00700 (B5D85_00700) - 108374..108682 (+) 309 Protein_89 hypothetical protein -
  B5D85_RS00705 (B5D85_00705) proC 108765..109535 (-) 771 WP_076639397.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16573.20 Da        Isoelectric Point: 10.3584

>NTDB_id=220892 B5D85_RS00680 WP_002986542.1 105137..105571(+) (comYF) [Streptococcus pyogenes strain STAB120304]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIGQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=220892 B5D85_RS00680 WP_002986542.1 105137..105571(+) (comYF) [Streptococcus pyogenes strain STAB120304]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAG
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGCTATTTGGAATATCACGTAGTCATATTCACATAGGGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.079

96.528

0.493
  comYF Streptococcus mutans UA159

50.36

96.528

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

45.985

95.139

0.437
  comGF/cglF Streptococcus mitis NCTC 12261

44.03

93.056

0.41
  comGF/cglF Streptococcus pneumoniae Rx1

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae D39

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae R6

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae TIGR4

43.609

92.361

0.403
  comGF/cglF Streptococcus mitis SK321

42.857

92.361

0.396

Multiple sequence alignment