Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   B1776_RS00270 Genome accession   NZ_CP019969
Coordinates   53549..54943 (+) Length   464 a.a.
NCBI ID   WP_011308691.1    Uniprot ID   A0A916NU34
Organism   Dehalococcoides mccartyi strain KBVC2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 48549..59943
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B1776_RS00265 (B1776_00265) - 50685..53159 (+) 2475 WP_011308690.1 ATP-dependent Clp protease ATP-binding subunit -
  B1776_RS00270 (B1776_00270) radA 53549..54943 (+) 1395 WP_011308691.1 DNA repair protein RadA Machinery gene
  B1776_RS00275 (B1776_00275) - 54930..55613 (+) 684 WP_078986731.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  B1776_RS00280 (B1776_00280) ispF 55619..56092 (+) 474 WP_011928579.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  B1776_RS00285 (B1776_00285) cysS 56096..57469 (+) 1374 WP_078986732.1 cysteine--tRNA ligase -
  B1776_RS00295 (B1776_00295) - 57685..58644 (+) 960 WP_228384443.1 tyrosine-type recombinase/integrase -
  B1776_RS00300 (B1776_00300) - 58856..59377 (+) 522 WP_078986733.1 NYN domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 49406.74 Da        Isoelectric Point: 6.5004

>NTDB_id=219311 B1776_RS00270 WP_011308691.1 53549..54943(+) (radA) [Dehalococcoides mccartyi strain KBVC2]
MDKSRNVYICSNCGHESLKWLGRCPGCQEWNTLEETTIAAPLGRKNAPARVISPAAELSSLNASDTTRRSLSISEFNRVL
GGGIVPGSLMLLGGEPGIGKSTLLLQVAASVAQSGGKVVYVSGEENPAQIKMRAQRLGISGEGLFLMAETDLNAILAQLS
VLCPSLVVIDSIQTVFLPELEAAPGAINQVRESALRLMQWAKSSGASVFITAHVTKEGNIAGPRILEHIVDVVMYFEGES
QSAYRLIRSVKNRFGSTNEVGIFEMKSEGLVEVANPSQIFLSNRQANTVGSAVTAVLEGSRPLLVEVQALTNTTSFGQPR
RTANGVDFNRTIMIAAVLSKRLSMRLGTQDIIVNATGGIRLDEPAADLAIALAIASSYRDIGVCPETIALGEIGLSGELR
TIPHLERRLSEASRLGFTRALVPAGANCQNININGIQIIAVSTVKEAIKLALTGVKTETEDVFE

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=219311 B1776_RS00270 WP_011308691.1 53549..54943(+) (radA) [Dehalococcoides mccartyi strain KBVC2]
ATGGATAAAAGCCGTAATGTTTATATCTGTTCCAACTGCGGACATGAGAGCCTGAAGTGGCTGGGGCGTTGCCCCGGCTG
TCAGGAATGGAATACTCTTGAAGAAACAACAATTGCTGCACCTCTCGGGCGTAAAAATGCACCTGCCCGGGTTATCAGCC
CGGCAGCCGAGCTTTCCAGTCTGAATGCATCAGATACTACCCGCCGGAGTCTATCTATTTCAGAGTTCAACCGGGTTTTG
GGCGGTGGAATAGTACCGGGTTCACTGATGCTTTTAGGTGGTGAACCGGGTATCGGCAAATCCACGCTCCTTCTTCAGGT
GGCTGCCTCGGTAGCCCAGAGCGGCGGCAAAGTGGTATATGTTTCCGGGGAAGAGAACCCCGCCCAGATAAAAATGCGCG
CTCAGCGTCTGGGTATCAGCGGCGAGGGGCTTTTCCTTATGGCTGAGACAGACCTTAATGCCATTCTAGCCCAGCTTTCA
GTCCTCTGCCCGTCATTGGTTGTTATAGACTCTATCCAGACTGTATTCCTGCCTGAGCTTGAGGCCGCACCCGGTGCTAT
AAATCAGGTGCGTGAATCAGCCCTCCGCCTGATGCAGTGGGCTAAGAGCAGCGGTGCCAGCGTATTTATCACCGCCCATG
TTACCAAAGAGGGCAACATCGCCGGGCCGCGCATACTGGAACACATAGTAGATGTGGTCATGTACTTTGAGGGCGAGTCC
CAGAGTGCTTACCGTTTGATACGTTCGGTCAAAAACCGCTTCGGTTCTACCAACGAAGTAGGCATATTTGAAATGAAAAG
TGAAGGATTGGTGGAAGTAGCCAATCCCTCGCAGATATTTTTATCTAATCGGCAGGCAAACACTGTTGGTTCAGCTGTAA
CAGCAGTGCTGGAGGGTAGCCGCCCTTTACTGGTAGAGGTGCAGGCACTCACCAATACCACCAGTTTCGGCCAGCCGCGC
CGCACGGCCAACGGGGTAGATTTTAACCGTACTATTATGATAGCCGCTGTTCTTTCCAAACGCCTTTCCATGCGGCTGGG
TACCCAGGACATAATAGTAAATGCCACCGGCGGTATTCGTCTGGACGAGCCGGCCGCAGATTTGGCTATTGCTTTGGCCA
TTGCCTCCAGTTACCGTGATATCGGGGTCTGCCCGGAAACCATAGCACTGGGTGAGATTGGCCTTTCAGGGGAACTGAGG
ACAATCCCTCATTTGGAAAGACGTCTTTCCGAGGCCAGCCGTTTGGGTTTTACGAGGGCTTTGGTACCTGCCGGTGCTAA
TTGCCAGAATATAAATATAAATGGTATCCAGATTATTGCGGTTTCAACTGTCAAAGAGGCTATTAAACTGGCGCTTACCG
GGGTAAAAACGGAGACCGAAGATGTTTTTGAATGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(66-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(335-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.218

98.707

0.496
  radA Streptococcus pneumoniae Rx1

48.578

98.491

0.478
  radA Streptococcus pneumoniae D39

48.578

98.491

0.478
  radA Streptococcus pneumoniae R6

48.578

98.491

0.478
  radA Streptococcus pneumoniae TIGR4

48.578

98.491

0.478
  radA Streptococcus mitis SK321

48.578

98.491

0.478
  radA Streptococcus mitis NCTC 12261

48.359

98.491

0.476

Multiple sequence alignment