Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   B1781_RS18095 Genome accession   NZ_CP019936
Coordinates   3816958..3818673 (+) Length   571 a.a.
NCBI ID   WP_078121000.1    Uniprot ID   -
Organism   Thiosocius teredinicola strain PMS-2146H.STBD.0c.01a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3811958..3823673
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B1781_RS18075 - 3812553..3814142 (+) 1590 WP_078120997.1 ATP-binding protein -
  B1781_RS18080 pilR 3814139..3815518 (+) 1380 WP_078120998.1 sigma-54 dependent transcriptional regulator Regulator
  B1781_RS23645 - 3815788..3815887 (+) 100 Protein_3590 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  B1781_RS18090 - 3816373..3816795 (+) 423 WP_078120999.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  B1781_RS18095 pilB 3816958..3818673 (+) 1716 WP_078121000.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B1781_RS18100 pilC 3818686..3819933 (+) 1248 WP_078121001.1 type II secretion system F family protein Machinery gene
  B1781_RS18105 pilD 3819975..3820811 (+) 837 WP_334223777.1 A24 family peptidase Machinery gene
  B1781_RS18110 coaE 3820804..3821409 (+) 606 WP_334223778.1 dephospho-CoA kinase -
  B1781_RS18115 zapD 3821465..3822250 (+) 786 WP_164513453.1 cell division protein ZapD -
  B1781_RS18120 yacG 3822247..3822438 (+) 192 WP_078121004.1 DNA gyrase inhibitor YacG -
  B1781_RS18125 - 3822466..3822888 (-) 423 WP_078121005.1 hypothetical protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62457.18 Da        Isoelectric Point: 4.8679

>NTDB_id=218972 B1781_RS18095 WP_078121000.1 3816958..3818673(+) (pilB) [Thiosocius teredinicola strain PMS-2146H.STBD.0c.01a]
MATTSPIVRLGGLARRLVQDGLLSEEEANSATKSASEAKLPLVSYLVSQRTVDAKAVALAASSEYGVPLFDLDAIDPETM
PGNLVEEKLVRKHHALPLIKRGNRLFLAVSDPTNHQGLDEIKFHTGLTTDAVLVEEDKLAAAINRALDAQDTSMTDLLDE
DLENLDISTGDEVDEDQDANLDVDDAPVVRYVNKILLDAINSGTSDIHFEPYEKVYRVRFRQDGMLHEKATPPINIAQRL
SARLKVMARMNIAERRVPQDGRIKMVLSRNRSIDFRVNTCPTLYGEKIVLRILDSSAAQLGVEALGFDDAQQRHFLEAIK
KPYGMVLVTGPTGSGKTVSLYTALNLLNQPEVNISTAEDPVEIQVAGINQVNVNPKTGLTFAEALRAFLRQDPDIVMVGE
IRDLETAEISVKAAQTGHLVLSTLHTNDAPQTLTRLANMGIPPFNIASSVNLILAQRLARRLCEHCKTVDEVPHDALIEE
GFTDEDVRDGLEVYKAVGCDKCTKGYKGRVGLFEVMPVSDAMGKLIMEGANSIQIRDLAKSEGLQDLRASGLAKVKAGIT
SLEEINRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=218972 B1781_RS18095 WP_078121000.1 3816958..3818673(+) (pilB) [Thiosocius teredinicola strain PMS-2146H.STBD.0c.01a]
ATGGCAACTACTAGCCCAATAGTCCGCCTCGGTGGTCTAGCACGACGTCTCGTGCAGGACGGCCTGCTGTCTGAAGAAGA
AGCAAACAGTGCCACCAAATCCGCCTCCGAGGCCAAACTGCCGCTGGTATCCTATTTGGTTAGCCAGCGAACCGTGGACG
CCAAGGCAGTCGCCCTTGCCGCGTCATCCGAATACGGCGTGCCACTGTTCGACCTCGACGCGATAGATCCGGAAACCATG
CCCGGTAATCTCGTCGAAGAAAAACTGGTACGCAAACACCACGCACTACCGTTGATCAAACGAGGGAATCGCCTTTTCCT
GGCGGTTTCTGACCCGACCAACCACCAAGGCCTCGACGAGATCAAATTTCATACGGGCCTAACGACCGACGCCGTTTTGG
TGGAGGAAGACAAACTCGCCGCGGCGATCAATCGTGCGCTGGATGCCCAAGACACCTCGATGACGGATCTTCTTGACGAA
GATCTCGAGAATCTGGACATCAGCACCGGCGACGAGGTCGACGAAGACCAGGACGCGAACCTGGATGTCGACGATGCGCC
GGTCGTGCGCTATGTGAACAAGATCCTGCTCGATGCGATCAATTCGGGCACGTCCGACATCCACTTCGAGCCCTACGAAA
AGGTCTACCGCGTGCGCTTCCGGCAGGACGGCATGCTGCACGAAAAAGCCACGCCGCCGATCAATATCGCACAACGCCTC
TCGGCTCGCCTCAAAGTTATGGCTCGGATGAATATCGCCGAGCGGCGGGTACCGCAGGACGGTCGTATCAAGATGGTATT
GTCGCGCAATCGCTCGATCGACTTTCGTGTCAACACCTGCCCGACGTTGTATGGCGAGAAAATCGTGCTGCGTATCCTCG
ATTCGTCGGCTGCGCAGCTGGGTGTCGAGGCTTTAGGGTTCGACGACGCCCAACAACGACATTTCCTCGAAGCAATCAAG
AAACCCTACGGCATGGTGCTGGTAACCGGCCCGACCGGTAGCGGTAAGACGGTCAGTCTCTATACGGCGCTGAATCTTCT
CAACCAGCCCGAAGTCAACATCTCGACCGCAGAAGACCCGGTCGAGATCCAGGTTGCCGGGATCAACCAGGTCAACGTCA
ACCCCAAGACCGGCCTGACGTTCGCCGAAGCGTTACGCGCGTTTCTCCGCCAAGACCCGGACATCGTCATGGTCGGTGAG
ATCCGTGACCTGGAAACGGCGGAGATCTCAGTAAAAGCGGCACAAACCGGTCACTTGGTCCTGTCGACACTGCACACCAA
CGATGCGCCCCAAACGCTGACGCGATTGGCGAACATGGGCATTCCACCGTTCAATATCGCCTCGTCGGTCAACCTGATCC
TTGCGCAGCGCTTGGCCCGCCGACTCTGCGAACATTGCAAGACAGTGGACGAAGTACCACACGATGCACTGATCGAAGAA
GGCTTTACCGACGAAGATGTCCGCGATGGATTGGAAGTCTACAAAGCGGTTGGGTGCGACAAGTGCACCAAAGGCTACAA
GGGCCGAGTCGGCCTGTTCGAGGTCATGCCGGTCAGTGATGCCATGGGCAAGCTGATTATGGAGGGTGCTAACTCCATTC
AAATTCGCGACTTGGCGAAGTCGGAAGGATTGCAAGACCTACGCGCCTCTGGCCTTGCAAAAGTGAAAGCCGGCATCACA
AGCCTCGAAGAAATCAACCGAGTCACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-151)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

58.507

100

0.59
  pilB Acinetobacter baumannii D1279779

53.846

100

0.539
  pilB Acinetobacter baylyi ADP1

52.696

100

0.531
  pilF Neisseria gonorrhoeae MS11

50

99.124

0.496
  pilB Vibrio cholerae strain A1552

51.002

96.147

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

48.036

98.074

0.471
  pilB Vibrio campbellii strain DS40M4

47.434

98.949

0.469
  pilB/pilB1 Synechocystis sp. PCC 6803

41.261

97.198

0.401
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.674

96.673

0.384
  pilF Thermus thermophilus HB27

38.324

98.249

0.377

Multiple sequence alignment