Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   B2G52_RS00560 Genome accession   NZ_CP019894
Coordinates   101469..102701 (+) Length   410 a.a.
NCBI ID   WP_003710974.1    Uniprot ID   E4ZBA3
Organism   Neisseria lactamica strain Y92-1009     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 96469..107701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B2G52_RS00540 (B2G52_00540) fabI 97501..98286 (-) 786 WP_002223367.1 enoyl-ACP reductase FabI -
  B2G52_RS00545 (B2G52_00545) dapD 98441..99262 (-) 822 WP_003713998.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
  B2G52_RS00550 (B2G52_00550) - 99272..99502 (-) 231 WP_078835829.1 hypothetical protein -
  B2G52_RS00555 (B2G52_00555) pgi 99472..101115 (-) 1644 WP_003714000.1 glucose-6-phosphate isomerase -
  B2G52_RS00560 (B2G52_00560) pilG 101469..102701 (+) 1233 WP_003710974.1 type 4 pilus assembly protein PilG Machinery gene
  B2G52_RS00565 (B2G52_00565) pilD 102775..103635 (+) 861 WP_003710975.1 prepilin peptidase Machinery gene
  B2G52_RS00570 (B2G52_00570) coaE 103637..104269 (+) 633 WP_003714003.1 dephospho-CoA kinase -
  B2G52_RS00575 (B2G52_00575) - 104262..104471 (+) 210 WP_019272186.1 DNA gyrase inhibitor YacG -
  B2G52_RS00580 (B2G52_00580) pilF 104556..106232 (-) 1677 WP_003714005.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B2G52_RS00585 (B2G52_00585) - 106480..106686 (-) 207 WP_003714007.1 hypothetical protein -
  B2G52_RS00595 (B2G52_00595) - 106867..107313 (-) 447 WP_003714009.1 DUF441 domain-containing protein -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 45246.23 Da        Isoelectric Point: 10.0085

>NTDB_id=218709 B2G52_RS00560 WP_003710974.1 101469..102701(+) (pilG) [Neisseria lactamica strain Y92-1009]
MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARKKLAKRGIRPLQVTRVKTSSKRKITQEDITVFTRQLS
TMIKAGLPLMQAFEIVARGHGNPSMTEMLMEIRSNVEQGSTLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAVY
KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTVMDMSDFFVSYGWMVLIALGFAI
YGFLKLKARSIKIQRRMDALLLRMPIFGDIVRKGTIARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRV
IQGLSMTSGMRATELFPNMMVQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIGTLLVAMYL
PLFNLGNVVG

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=218709 B2G52_RS00560 WP_003710974.1 101469..102701(+) (pilG) [Neisseria lactamica strain Y92-1009]
ATGGCTAAAAATGGGGGATTTTCTTTGTTCGCAAAGAAAGAAAAACGCTTTATCTTTGAAGGCAGGCATTCCGCCTCCGA
CAAACTGGTCAACGGCGAAGTATCTGCGTTTACCGAAGAAGAGGCGCGCAAAAAACTCGCCAAACGCGGCATCCGCCCGC
TGCAGGTTACCCGTGTGAAAACAAGCTCCAAGCGCAAAATCACACAAGAAGACATCACCGTCTTCACCCGCCAGCTGTCC
ACGATGATTAAAGCGGGCCTGCCGCTGATGCAGGCATTTGAAATCGTGGCGCGCGGACACGGCAACCCGTCTATGACGGA
AATGCTGATGGAAATCCGCAGCAATGTGGAACAGGGCAGCACGTTGAGCCGCGCATTCTCGAACCACCCTAAATATTTCG
ACCGCTTCTACTGCAACCTGGTTGCGGCGGGCGAAACGGGCGGCGTGTTGGAAAGCCTGTTGGACAAATTGGCAGTCTAT
AAAGAAAAAACCCAGGCCATCCGCAAAAAGGTAAAAACCGCGCTGACCTATCCGGTATCCGTCATCGCCGTCGCCATCGG
TTTGGTATTCGTGATGATGATTTTCGTACTGCCCGCCTTTAAAGAGGTTTACGCCAATATGGGCGCGGAGCTTCCCGCAC
TGACCCAAACAGTGATGGATATGTCCGACTTTTTCGTCTCATACGGCTGGATGGTGCTGATCGCACTCGGCTTTGCCATA
TACGGCTTCCTCAAATTGAAGGCGCGTTCGATTAAAATCCAACGGCGTATGGATGCCCTGCTGCTGCGTATGCCGATTTT
CGGAGACATCGTCCGTAAAGGGACGATTGCCCGCTGGGGCAGGACGACGGCGACACTGATTGCGGCAGGCGTGCCTTTGG
TCGATGTATTGGACTCCACTGCCGGCGCGGCGGGCAATTTAATCTATGAAGAAGCCACCCGGGAAATCCGTACGCGCGTC
ATCCAAGGCCTGTCCATGACTTCCGGGATGCGTGCGACGGAACTGTTCCCCAATATGATGGTGCAGATGTCCTCCATCGG
CGAGGAATCGGGTTCTTTGGACGATATGCTCAACAAAGCCGCCGAATTTTATGAAGACGAGGTGGACAATGCGGTCGGTC
GGCTGTCCGCTATGATGGAGCCGATCATTATCGTGATTTTAGGCTTGGTCATCGGTACGCTTCTGGTCGCTATGTATCTG
CCGCTGTTTAATTTGGGCAACGTGGTCGGCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)

Type II secretion system (T2SS), protein F (T2SSF)

(281-401)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E4ZBA3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

97.8

99.756

0.976
  pilG Neisseria meningitidis 44/76-A

97.066

99.756

0.968
  pilC Acinetobacter baumannii D1279779

47.487

97.073

0.461
  pilC Acinetobacter baylyi ADP1

46.115

97.317

0.449
  pilC Legionella pneumophila strain ERS1305867

45.34

96.829

0.439
  pilC Pseudomonas stutzeri DSM 10701

44.527

98.049

0.437
  pilC Vibrio cholerae strain A1552

40.943

98.293

0.402
  pilC Vibrio campbellii strain DS40M4

39.066

99.268

0.388

Multiple sequence alignment