Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   B2G52_RS00580 Genome accession   NZ_CP019894
Coordinates   104556..106232 (-) Length   558 a.a.
NCBI ID   WP_003714005.1    Uniprot ID   -
Organism   Neisseria lactamica strain Y92-1009     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 99556..111232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B2G52_RS00560 (B2G52_00560) pilG 101469..102701 (+) 1233 WP_003710974.1 type 4 pilus assembly protein PilG Machinery gene
  B2G52_RS00565 (B2G52_00565) pilD 102775..103635 (+) 861 WP_003710975.1 prepilin peptidase Machinery gene
  B2G52_RS00570 (B2G52_00570) coaE 103637..104269 (+) 633 WP_003714003.1 dephospho-CoA kinase -
  B2G52_RS00575 (B2G52_00575) - 104262..104471 (+) 210 WP_019272186.1 DNA gyrase inhibitor YacG -
  B2G52_RS00580 (B2G52_00580) pilF 104556..106232 (-) 1677 WP_003714005.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B2G52_RS00585 (B2G52_00585) - 106480..106686 (-) 207 WP_003714007.1 hypothetical protein -
  B2G52_RS00595 (B2G52_00595) - 106867..107313 (-) 447 WP_003714009.1 DUF441 domain-containing protein -
  B2G52_RS00600 (B2G52_00600) - 107318..108292 (-) 975 WP_003714011.1 polyprenyl synthetase family protein -
  B2G52_RS00610 (B2G52_00610) rplU 108516..108824 (+) 309 WP_002216394.1 50S ribosomal protein L21 -
  B2G52_RS00615 (B2G52_00615) rpmA 108850..109122 (+) 273 WP_002212328.1 50S ribosomal protein L27 -
  B2G52_RS00620 (B2G52_00620) ubiM 109356..110540 (+) 1185 WP_003714013.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -
  B2G52_RS00625 (B2G52_00625) rpmG 110729..110884 (-) 156 WP_003689824.1 50S ribosomal protein L33 -
  B2G52_RS00630 (B2G52_00630) rpmB 110916..111149 (-) 234 WP_002216391.1 50S ribosomal protein L28 -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62033.12 Da        Isoelectric Point: 5.4107

>NTDB_id=218711 B2G52_RS00580 WP_003714005.1 104556..106232(-) (pilF) [Neisseria lactamica strain Y92-1009]
MSVGLLRTLVQNQVVTVEQAEHYYKESQAGKEVLPMLFSDGVISPKSLAALIARVFSYSVLDLRHYPRHRVLMGVLTEEQ
MVEFHCVPVFRRDNKVFFAVSDPTQMPQIQKTVSAAGIAVELVIVEDDQLAGLLDWVGSRSTSLLQELGEEQEEEESHTL
YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASRIKVMSRLDISEKR
IPQDGRMQLTFQKGGKPIDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNEKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVGFTDEDLAKDWKLY
HAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=218711 B2G52_RS00580 WP_003714005.1 104556..106232(-) (pilF) [Neisseria lactamica strain Y92-1009]
ATGAGCGTAGGTTTGCTGAGAACTTTGGTTCAAAACCAAGTGGTTACTGTCGAACAGGCCGAACACTATTATAAGGAGTC
GCAGGCGGGTAAAGAAGTGTTGCCGATGCTGTTTTCAGACGGTGTCATTTCGCCTAAATCCCTTGCGGCACTGATTGCCA
GGGTGTTCAGTTATTCGGTTCTTGATTTGCGCCATTATCCGCGCCACAGGGTGCTGATGGGGGTGTTGACGGAAGAGCAG
ATGGTGGAGTTCCACTGTGTGCCGGTTTTCCGTCGGGACAATAAAGTATTTTTTGCGGTTTCCGATCCGACACAGATGCC
GCAAATTCAGAAAACCGTGTCTGCCGCAGGGATTGCGGTTGAGTTGGTCATTGTCGAGGATGACCAGTTGGCGGGTTTGC
TCGATTGGGTGGGGTCGAGATCGACATCGCTGCTTCAGGAGCTTGGGGAGGAGCAGGAGGAAGAGGAAAGCCACACCCTG
TATATCGACAATGAGGAGGCGGAGGACGGCCCTGTTCCGAGGTTTATCCATAAAACTTTGTCGGATGCCTTGCGCAGCGG
GGCATCGGACATTCATTTTGAGTTTTACGAACACAATGCCCGTATCCGTTTCCGCGTGGACGGGCAGCTTCGCGAGGTGG
TTCAGCCGCCTATTGCGGTAAGGGGGCAGCTTGCTTCCCGGATTAAGGTGATGTCGCGTTTGGATATTTCCGAAAAACGC
ATACCGCAGGACGGCAGGATGCAGCTGACCTTTCAAAAGGGCGGCAAGCCTATCGATTTCCGCGTCAGCACTTTGCCGAC
GCTGTTTGGCGAAAAGGTTGTGATGCGGATTTTGAACTCCGATGCCGCGTCTTTGAATATCGACCAGCTCGGTTTTGAGC
CGTTTCAGAAAAAATTGCTGTTGGAAGCGATTCATCGTCCTTACGGTATGGTGCTGGTAACCGGCCCGACGGGTTCGGGT
AAGACGGTGTCGCTCTATACCTGTTTGAATATTTTGAATACGGAGTCGGTAAATATTGCAACGGCGGAAGACCCTGCCGA
GATTAACCTGCCAGGCATCAATCAGGTCAACGTCAATGAGAAGCAGGGTCTGACTTTTGCCGCTGCTTTAAAGTCTTTCC
TGCGTCAGGATCCGGACATCATTATGGTCGGTGAGATTCGTGATTTGGAAACTGCCGATATTGCGATTAAGGCGGCACAA
ACAGGGCATATGGTGTTTTCCACCCTGCACACCAATAATGCGCCGGCGACGTTGTCGCGTATGCTGAATATGGGTGTCGC
GCCGTTTAATATTGCCAGTTCGGTCAGCCTGATTATGGCGCAGCGTCTTTTACGCAGGTTGTGTTCGAGTTGCAAACAGG
AAGTGGAACGTCCGTCTGCTTCTGCTTTGAAGGAAGTCGGCTTCACCGATGAGGATCTTGCAAAAGATTGGAAACTTTAC
CATGCCGTCGGTTGCGACCGTTGCCGGGGTCAGGGTTACAAGGGGCGTGCGGGCGTGTATGAGGTTATGCCCATCAGCGA
AGAAATGCAGCGTGTGATTATGAACAACGGTACGGAAGTGGATATTTTGGACGTTGCCTATAAGGAGGGTATGGTGGATT
TGCGCCGGGCCGGTATTTTGAAAGTTATGCAGGGCATTACTTCATTGGAAGAGGTAACGGCAAATACCAACGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)

Type II/IV secretion system protein (T2SSE)

(178-446)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

98.029

100

0.98
  pilB Acinetobacter baumannii D1279779

50.355

100

0.509
  pilB Acinetobacter baylyi ADP1

50.356

100

0.507
  pilB Legionella pneumophila strain ERS1305867

47.703

100

0.484
  pilB Vibrio cholerae strain A1552

45.552

100

0.459
  pilB Vibrio parahaemolyticus RIMD 2210633

45.39

100

0.459
  pilB Vibrio campbellii strain DS40M4

43.972

100

0.444
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.179

96.057

0.376

Multiple sequence alignment