Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   Mag101_RS12980 Genome accession   NZ_CP019650
Coordinates   3112595..3113806 (+) Length   403 a.a.
NCBI ID   WP_077405767.1    Uniprot ID   A0A1Q2M6T3
Organism   Microbulbifer agarilyticus strain GP101     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3107595..3118806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Mag101_RS12955 (Mag101_13020) - 3107659..3108057 (+) 399 WP_077405751.1 DUF2750 domain-containing protein -
  Mag101_RS12960 (Mag101_13025) - 3108109..3108357 (-) 249 WP_077405754.1 YdcH family protein -
  Mag101_RS12965 (Mag101_13030) - 3108440..3109870 (-) 1431 WP_077405757.1 O-antigen ligase family protein -
  Mag101_RS18260 (Mag101_13035) - 3109962..3110561 (-) 600 WP_269466557.1 pilin -
  Mag101_RS12975 (Mag101_13040) pilB 3110846..3112558 (+) 1713 WP_077405764.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Mag101_RS12980 (Mag101_13045) pilC 3112595..3113806 (+) 1212 WP_077405767.1 type II secretion system F family protein Machinery gene
  Mag101_RS12985 (Mag101_13050) - 3113819..3114703 (+) 885 WP_077405770.1 prepilin peptidase -
  Mag101_RS12990 (Mag101_13055) coaE 3114707..3115321 (+) 615 WP_077405773.1 dephospho-CoA kinase -
  Mag101_RS12995 (Mag101_13060) yacG 3115331..3115552 (+) 222 WP_077405776.1 DNA gyrase inhibitor YacG -
  Mag101_RS13000 (Mag101_13065) - 3115576..3116088 (-) 513 WP_077405779.1 protein disulfide oxidoreductase -
  Mag101_RS13005 (Mag101_13070) tsaA 3116085..3116837 (-) 753 WP_077405782.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  Mag101_RS13010 (Mag101_13075) - 3116834..3118291 (-) 1458 WP_077405785.1 NAD-dependent succinate-semialdehyde dehydrogenase -

Sequence

Protein


Download         Length: 403 a.a.        Molecular weight: 43452.10 Da        Isoelectric Point: 9.2570

>NTDB_id=217571 Mag101_RS12980 WP_077405767.1 3112595..3113806(+) (pilC) [Microbulbifer agarilyticus strain GP101]
MANSAAVAYVYKGVDAKGNRVEGEISGTNPALIKAQLRRQGIIASKVQKKPKPLFGASKKVKPADIAVFTRQMATMMKAG
VPLVQSFEIVADGLDNEGLKDLIFKIRDEVASGTAFADALRKHPVYFDDLFCNLVASGEQSGALETMLDRIATYKEKTES
LKAKIKKAMTYPIAVLVVAVIVTSILLIKVVPQFADTFSSFGANLPAFTLFVLGISEWMQANWLIAFITLVVGIGGTLEV
KKRNKNVANFFDRLMLKIPILGQITYNSIAARFARTLSTTFAAGVPLIDALKSVAGATGNVVYEEATYKIRDSVATGIPL
NGAMRSSGLYPTMLVQMAAIGEESGALDEMLSKAADFYEEAVDNMVDNLTTLLEPMIMAILGILVGGLMIAMYLPIFQLG
QVI

Nucleotide


Download         Length: 1212 bp        

>NTDB_id=217571 Mag101_RS12980 WP_077405767.1 3112595..3113806(+) (pilC) [Microbulbifer agarilyticus strain GP101]
ATGGCCAATTCCGCCGCAGTCGCCTACGTATATAAGGGCGTGGACGCCAAGGGCAACAGGGTAGAGGGTGAGATTAGTGG
GACCAATCCTGCGCTAATCAAGGCGCAATTGCGTCGTCAAGGAATCATCGCCAGCAAGGTGCAGAAGAAGCCGAAACCAT
TATTCGGTGCCAGCAAAAAGGTCAAACCGGCCGATATCGCAGTTTTTACCCGGCAAATGGCGACTATGATGAAAGCCGGT
GTTCCCCTTGTGCAAAGCTTTGAAATCGTCGCTGATGGCTTAGACAATGAAGGACTTAAGGATCTGATATTTAAAATCCG
CGACGAAGTCGCTTCAGGTACTGCCTTTGCTGATGCACTACGTAAACACCCAGTGTACTTTGATGACCTGTTCTGTAATT
TAGTTGCGTCAGGTGAGCAATCCGGTGCCCTAGAAACCATGCTCGACCGAATAGCGACCTACAAAGAGAAAACTGAGTCG
CTTAAAGCGAAAATTAAGAAAGCAATGACGTATCCAATTGCCGTTCTGGTGGTTGCCGTCATTGTTACTTCTATTCTGCT
AATCAAAGTAGTCCCCCAATTTGCCGACACTTTCTCTAGCTTCGGCGCGAACTTGCCAGCTTTTACTTTGTTCGTTTTGG
GAATTTCAGAATGGATGCAAGCCAACTGGTTAATTGCATTTATTACCCTAGTGGTAGGTATAGGCGGGACTTTAGAGGTT
AAAAAACGCAATAAAAATGTTGCAAACTTCTTTGACAGATTAATGCTCAAAATCCCTATTTTGGGTCAAATAACCTACAA
CTCGATTGCGGCCCGTTTTGCGCGTACACTATCAACCACTTTTGCTGCAGGTGTACCGCTAATCGACGCACTCAAGTCTG
TAGCCGGCGCCACCGGTAACGTCGTCTATGAGGAGGCGACATACAAGATTCGAGATTCAGTTGCGACCGGTATTCCGCTG
AACGGTGCGATGCGCTCGTCGGGACTCTACCCGACGATGCTGGTACAAATGGCCGCTATCGGAGAAGAATCAGGTGCACT
CGATGAGATGCTCAGTAAAGCAGCAGATTTTTATGAAGAAGCAGTGGATAACATGGTAGACAACCTCACCACACTTCTTG
AACCCATGATTATGGCTATTTTGGGAATTTTGGTGGGTGGACTAATGATCGCCATGTATCTTCCGATCTTCCAGCTCGGT
CAAGTTATTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(69-192)

Type II secretion system (T2SS), protein F (T2SSF)

(273-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Q2M6T3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

64.557

98.015

0.633
  pilC Acinetobacter baylyi ADP1

59.95

98.511

0.591
  pilC Acinetobacter baumannii D1279779

58.942

98.511

0.581
  pilC Legionella pneumophila strain ERS1305867

56.281

98.759

0.556
  pilG Neisseria gonorrhoeae MS11

41.919

98.263

0.412
  pilG Neisseria meningitidis 44/76-A

41.667

98.263

0.409
  pilC Vibrio cholerae strain A1552

41.667

98.263

0.409
  pilC Vibrio campbellii strain DS40M4

40

99.256

0.397
  pilC Thermus thermophilus HB27

36.364

100

0.367

Multiple sequence alignment