Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Mag101_RS12975 Genome accession   NZ_CP019650
Coordinates   3110846..3112558 (+) Length   570 a.a.
NCBI ID   WP_077405764.1    Uniprot ID   A0A1Q2M6X1
Organism   Microbulbifer agarilyticus strain GP101     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3105846..3117558
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Mag101_RS12945 (Mag101_13010) - 3106563..3107228 (-) 666 WP_077405745.1 peroxiredoxin-like family protein -
  Mag101_RS18330 (Mag101_13015) - 3107270..3107590 (+) 321 WP_077405748.1 DUF333 domain-containing protein -
  Mag101_RS12955 (Mag101_13020) - 3107659..3108057 (+) 399 WP_077405751.1 DUF2750 domain-containing protein -
  Mag101_RS12960 (Mag101_13025) - 3108109..3108357 (-) 249 WP_077405754.1 YdcH family protein -
  Mag101_RS12965 (Mag101_13030) - 3108440..3109870 (-) 1431 WP_077405757.1 O-antigen ligase family protein -
  Mag101_RS18260 (Mag101_13035) - 3109962..3110561 (-) 600 WP_269466557.1 pilin -
  Mag101_RS12975 (Mag101_13040) pilB 3110846..3112558 (+) 1713 WP_077405764.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Mag101_RS12980 (Mag101_13045) pilC 3112595..3113806 (+) 1212 WP_077405767.1 type II secretion system F family protein Machinery gene
  Mag101_RS12985 (Mag101_13050) - 3113819..3114703 (+) 885 WP_077405770.1 prepilin peptidase -
  Mag101_RS12990 (Mag101_13055) coaE 3114707..3115321 (+) 615 WP_077405773.1 dephospho-CoA kinase -
  Mag101_RS12995 (Mag101_13060) yacG 3115331..3115552 (+) 222 WP_077405776.1 DNA gyrase inhibitor YacG -
  Mag101_RS13000 (Mag101_13065) - 3115576..3116088 (-) 513 WP_077405779.1 protein disulfide oxidoreductase -
  Mag101_RS13005 (Mag101_13070) tsaA 3116085..3116837 (-) 753 WP_077405782.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62287.12 Da        Isoelectric Point: 5.0942

>NTDB_id=217570 Mag101_RS12975 WP_077405764.1 3110846..3112558(+) (pilB) [Microbulbifer agarilyticus strain GP101]
MSSIPLSGLAKRLVIDKAIDEPTAQSAVKAAKREGQSFAQHAVEAKLIKSRELANIATQAFGSPLFDLSSYNFDLLPKDV
LDEKLITKHFALPLYKRANRLFVAVADPTNLAGLDEINFNTGLNTEAVLVEADKLAKAIESYLANSGDISGGLEGLDDDD
LDSLNIDGGEQEREDDSDAVGDEAPVVRFVNKVLLDAIRTGASDIHFEPYEKSYRVRLRTDGVLHEVARPPIQLATRLSA
RLKVMSKMDISERRVPQDGRIKMKLSKTKAIDFRVNSLPTLWGEKIVLRILDPSSAKLGIDALGYEEEQKQLYLDALAQP
QGMILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPVEINLEGINQVNVSAKVGLNFAEALRSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPTFNIATSVSVIIAQRLARRLCGECKKPAELPEEVLADEGF
DAVTIPKSEWQIYQPVGCEHCSKGYKGRVGVYEVVRITDGISRIIMEGGNSIQIADQAKKEGFNNLRTSALRKVVMGVTS
LEEANRVTKD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=217570 Mag101_RS12975 WP_077405764.1 3110846..3112558(+) (pilB) [Microbulbifer agarilyticus strain GP101]
ATGAGCAGCATCCCTCTGAGCGGACTAGCCAAGCGACTGGTCATCGACAAGGCTATCGACGAGCCCACCGCACAATCCGC
CGTAAAGGCTGCCAAACGCGAGGGACAGAGTTTTGCCCAACACGCAGTAGAGGCAAAACTGATCAAGAGCCGGGAATTGG
CCAACATAGCCACCCAAGCGTTCGGCTCCCCTCTATTCGACCTGTCTAGCTACAACTTCGATTTGTTGCCAAAGGATGTA
CTCGACGAAAAGCTGATCACCAAGCATTTCGCACTTCCACTCTATAAGCGAGCTAACAGGCTGTTTGTTGCTGTCGCGGA
CCCAACCAACCTTGCCGGCCTTGACGAGATTAACTTCAATACCGGCCTGAACACTGAAGCGGTACTGGTCGAAGCGGACA
AGCTTGCCAAGGCAATCGAAAGCTATTTGGCAAACAGCGGTGATATTAGTGGTGGCTTGGAAGGGCTAGACGACGATGAT
CTCGACAGCCTCAACATCGATGGAGGCGAGCAAGAGCGAGAGGATGACAGCGACGCCGTAGGAGACGAAGCCCCCGTTGT
ACGTTTTGTAAACAAGGTCCTGCTCGATGCCATCCGCACAGGGGCGTCAGATATACACTTCGAGCCATATGAGAAGTCCT
ATCGAGTGCGCCTGCGCACAGATGGCGTGCTGCACGAAGTTGCACGCCCTCCAATTCAGCTCGCTACCAGATTGTCAGCA
CGCCTCAAAGTAATGTCCAAAATGGATATTTCCGAACGCCGGGTACCTCAGGATGGCCGAATAAAGATGAAGCTATCCAA
AACCAAGGCCATCGACTTTCGAGTCAACAGCCTACCCACCCTGTGGGGTGAGAAGATTGTATTGCGAATTCTTGACCCAT
CCTCCGCAAAGCTCGGTATTGATGCTCTGGGCTACGAAGAAGAGCAAAAACAGCTCTATCTCGATGCTCTCGCACAACCG
CAGGGGATGATTTTGGTAACTGGCCCCACGGGCTCGGGTAAAACGGTATCTCTGTATACCGGCCTGAATATACTTAATAC
CCCAGAAAGAAACATTTCCACGGCGGAAGACCCGGTGGAAATTAACCTTGAAGGCATCAACCAGGTTAACGTCTCCGCCA
AGGTAGGACTAAATTTCGCCGAGGCTTTACGCTCCTTCTTGCGGCAAGACCCCGATATTGTGATGGTAGGTGAGATAAGG
GATTTGGAAACCGCAGAGATCGCCATCAAAGCGGCGCAAACAGGTCACTTGGTGTTATCCACCTTGCACACCAATTCCGC
CCCAGAGACTTTGACTCGCTTGATGAATATGGGCGTTCCCACATTCAATATCGCAACGTCTGTCAGTGTAATTATTGCTC
AACGCTTGGCACGGAGACTTTGTGGAGAGTGTAAAAAACCCGCAGAACTACCAGAAGAAGTGTTGGCCGATGAAGGCTTT
GATGCGGTCACAATTCCAAAGTCTGAATGGCAAATATACCAACCTGTGGGCTGTGAACATTGTTCCAAAGGCTATAAAGG
ACGCGTGGGTGTGTATGAAGTGGTTCGCATTACCGACGGAATCTCACGCATTATAATGGAGGGAGGCAACTCCATTCAAA
TTGCAGATCAAGCCAAAAAGGAAGGCTTTAATAATCTCCGCACTTCGGCGCTGCGCAAGGTAGTAATGGGTGTGACTAGC
TTGGAAGAAGCTAATCGAGTAACCAAAGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Q2M6X1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.984

99.825

0.609
  pilB Acinetobacter baylyi ADP1

60.177

99.123

0.596
  pilB Legionella pneumophila strain ERS1305867

54.482

99.825

0.544
  pilB Vibrio campbellii strain DS40M4

54.396

95.789

0.521
  pilB Vibrio parahaemolyticus RIMD 2210633

53.818

96.491

0.519
  pilB Vibrio cholerae strain A1552

53.285

96.14

0.512
  pilF Neisseria gonorrhoeae MS11

50.379

92.632

0.467
  pilB/pilB1 Synechocystis sp. PCC 6803

39.123

100

0.423

Multiple sequence alignment