Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   B0W48_RS10490 Genome accession   NZ_CP019628
Coordinates   2363556..2364782 (-) Length   408 a.a.
NCBI ID   WP_077536900.1    Uniprot ID   A0A1Q2GYU8
Organism   Pseudoalteromonas aliena strain EH1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2358556..2369782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B0W48_RS10465 (B0W48_10515) - 2359119..2359607 (+) 489 WP_077536895.1 Spy/CpxP family protein refolding chaperone -
  B0W48_RS10470 (B0W48_10520) yacG 2359680..2359910 (-) 231 WP_077536896.1 DNA gyrase inhibitor YacG -
  B0W48_RS10475 (B0W48_10525) pilB 2360217..2361893 (-) 1677 WP_077536897.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B0W48_RS10480 (B0W48_10530) coaE 2362009..2362653 (-) 645 WP_077536898.1 dephospho-CoA kinase -
  B0W48_RS10485 (B0W48_10535) pilD 2362655..2363539 (-) 885 WP_077536899.1 prepilin peptidase Machinery gene
  B0W48_RS10490 (B0W48_10540) pilC 2363556..2364782 (-) 1227 WP_077536900.1 type II secretion system F family protein Machinery gene
  B0W48_RS10495 (B0W48_10545) - 2364888..2365331 (-) 444 WP_077536901.1 pilin -
  B0W48_RS10500 (B0W48_10550) nadC 2365998..2366843 (-) 846 WP_077536902.1 carboxylating nicotinate-nucleotide diphosphorylase -
  B0W48_RS10505 (B0W48_10555) ampD 2367276..2367830 (+) 555 WP_077536903.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  B0W48_RS10510 (B0W48_10560) ampE 2367844..2368695 (+) 852 WP_077536904.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44383.55 Da        Isoelectric Point: 9.7444

>NTDB_id=217402 B0W48_RS10490 WP_077536900.1 2363556..2364782(-) (pilC) [Pseudoalteromonas aliena strain EH1]
MAKKQENKGLDTFKWVGVSARGKRLEGELTGTSIALVKAQLRKQGVTPSKVKRKPKPLFGVHTQKITPKDIALVTRQIAT
MLMAGVPLIQSIEMIGSGATNKSVGKLMEAIAGDVKAGQPLSTALRKYPRYFDDLYCDLVESGEQSGALDKIFDRVAIYK
EKSEALKSKIKKAMFYPMSVLAIAGIVTSILLIFVVPQFQDIFAGFGAELPPFTLFVIAISEFMQEYWWIILIIMVAAGW
IFKEAKLKSLKLRDANDRVILKLPVIGMILNKAAVARYARTLSTTFAAGVPLIEALDSAAGASGNAVYRYAILDIKAEVA
SGNQMNWAMRNSKIFPDMVIQMVAIGEESGALDDMLAKVATIYEQEVDDAVDGLSSLLEPLIMAVLGVLVGGLIVAMYLP
IFQLGSVI

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=217402 B0W48_RS10490 WP_077536900.1 2363556..2364782(-) (pilC) [Pseudoalteromonas aliena strain EH1]
ATGGCGAAAAAACAAGAAAATAAGGGGCTCGATACTTTTAAATGGGTTGGTGTTAGCGCTCGAGGTAAACGCCTTGAAGG
AGAGCTTACAGGTACAAGTATAGCACTTGTTAAAGCACAGCTTCGCAAGCAAGGGGTCACCCCATCAAAAGTTAAACGTA
AACCAAAACCTTTATTCGGCGTACACACGCAAAAAATAACTCCAAAAGACATCGCATTAGTGACCCGACAAATTGCCACT
ATGCTCATGGCAGGCGTCCCCCTTATACAGTCGATAGAAATGATTGGTTCAGGCGCAACTAATAAAAGCGTTGGCAAGCT
AATGGAAGCGATTGCAGGTGACGTAAAAGCAGGCCAACCTCTGTCTACTGCACTTAGAAAATATCCACGTTATTTTGATG
ACTTATATTGTGATTTAGTTGAGTCGGGCGAGCAATCGGGTGCACTTGATAAAATATTTGATCGTGTTGCAATTTATAAA
GAAAAATCTGAAGCGCTTAAATCAAAAATTAAAAAAGCCATGTTTTACCCTATGTCTGTATTAGCTATTGCAGGGATTGT
TACTTCTATTTTGCTCATATTTGTAGTGCCGCAATTTCAGGATATTTTTGCTGGTTTTGGCGCTGAGCTGCCTCCATTTA
CTTTATTTGTGATTGCTATTTCTGAATTTATGCAGGAATACTGGTGGATTATTCTAATAATAATGGTCGCAGCTGGCTGG
ATTTTTAAAGAAGCCAAGCTTAAAAGCTTAAAACTGCGTGATGCCAACGATAGGGTTATATTAAAGCTACCTGTAATAGG
TATGATTTTAAATAAAGCAGCCGTTGCGCGTTACGCGCGTACACTTTCTACCACGTTTGCAGCTGGTGTGCCGTTAATTG
AGGCTCTTGATTCTGCGGCGGGTGCTTCAGGTAATGCGGTATATCGCTATGCTATTTTAGATATAAAAGCAGAGGTTGCC
TCTGGTAACCAGATGAACTGGGCTATGCGCAATTCAAAAATATTCCCCGATATGGTAATCCAAATGGTTGCTATTGGTGA
AGAGTCTGGCGCACTTGATGATATGCTGGCCAAAGTAGCCACTATTTATGAGCAAGAAGTAGATGATGCAGTTGATGGCT
TATCTAGCTTATTAGAGCCATTAATTATGGCTGTACTCGGTGTGCTGGTCGGTGGTTTAATTGTTGCTATGTACCTTCCT
ATTTTCCAACTTGGTTCTGTTATTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(279-400)

Type II secretion system (T2SS), protein F (T2SSF)

(75-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Q2GYU8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

61.111

97.059

0.593
  pilC Acinetobacter baumannii D1279779

56.5

98.039

0.554
  pilC Legionella pneumophila strain ERS1305867

54.887

97.794

0.537
  pilC Acinetobacter baylyi ADP1

54.912

97.304

0.534
  pilC Vibrio cholerae strain A1552

44.638

98.284

0.439
  pilG Neisseria gonorrhoeae MS11

43.077

95.588

0.412
  pilG Neisseria meningitidis 44/76-A

42.821

95.588

0.409
  pilC Vibrio campbellii strain DS40M4

41.294

98.529

0.407

Multiple sequence alignment