Detailed information NTG0217401

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: B0W48_RS10485
• Genome accession: NZ_CP019628
• Coordinates: 2362655..2363539 (-)
• Length: 294 a.a.
• NCBI ID: WP_077536899.1
• Uniprot ID: A0A1Q2GYI3
• Organism: Pseudoalteromonas aliena strain EH1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2357655..2368539

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B0W48_RS10460 (B0W48_10510)	-	2358242..2358931 (-)	690	WP_077536894.1	response regulator	-
B0W48_RS10465 (B0W48_10515)	-	2359119..2359607 (+)	489	WP_077536895.1	Spy/CpxP family protein refolding chaperone	-
B0W48_RS10470 (B0W48_10520)	yacG	2359680..2359910 (-)	231	WP_077536896.1	DNA gyrase inhibitor YacG	-
B0W48_RS10475 (B0W48_10525)	pilB	2360217..2361893 (-)	1677	WP_077536897.1	type IV-A pilus assembly ATPase PilB	Machinery gene
B0W48_RS10480 (B0W48_10530)	coaE	2362009..2362653 (-)	645	WP_077536898.1	dephospho-CoA kinase	-
B0W48_RS10485 (B0W48_10535)	pilD	2362655..2363539 (-)	885	WP_077536899.1	prepilin peptidase	Machinery gene
B0W48_RS10490 (B0W48_10540)	pilC	2363556..2364782 (-)	1227	WP_077536900.1	type II secretion system F family protein	Machinery gene
B0W48_RS10495 (B0W48_10545)	-	2364888..2365331 (-)	444	WP_077536901.1	pilin	-
B0W48_RS10500 (B0W48_10550)	nadC	2365998..2366843 (-)	846	WP_077536902.1	carboxylating nicotinate-nucleotide diphosphorylase	-
B0W48_RS10505 (B0W48_10555)	ampD	2367276..2367830 (+)	555	WP_077536903.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-

Sequence

Protein

• Length: 294 a.a.
• Molecular weight: 32261.53 Da
• Isoelectric Point: 7.5068

>NTDB_id=217401 B0W48_RS10485 WP_077536899.1 2362655..2363539(-) (pilD) [Pseudoalteromonas aliena strain EH1]
MQSIIEVMQSQLWFYLTTIGLVSLCVGSFLNVLIYRLPLMMQKEWQSECRILLEGDLKNSAPLNATPFNLVKPNSTCPHC
KTAIKPWQNIPIISWLALKGKCASCQNTIAARYPLVEAITALLSLAVAYTVGANEQALLYILITWALVALTFIDIDHMLL
PDQLTLPLVWLALIASVMGYTISPSDAIIGAACGYLSLWSVFWLFKLLTGKEGMGYGDFKLLAVFGALLGWQSLLTIILL
SSVVGAIIGITLLSIQGKDKATPIPFGPYLAIAGWIAMLWGNQLQTMYLNLVGY

Nucleotide

• Length: 885 bp

>NTDB_id=217401 B0W48_RS10485 WP_077536899.1 2362655..2363539(-) (pilD) [Pseudoalteromonas aliena strain EH1]
ATGCAAAGTATTATAGAAGTAATGCAAAGCCAATTGTGGTTTTACTTAACCACCATTGGTTTAGTTAGTTTATGTGTAGG
TAGCTTTTTAAATGTACTCATTTATCGGCTACCGTTAATGATGCAAAAAGAATGGCAAAGTGAATGCCGTATTTTATTAG
AGGGTGACCTTAAAAATAGCGCGCCCCTAAACGCCACGCCATTTAATTTAGTAAAACCCAACTCAACCTGCCCGCACTGT
AAAACCGCAATTAAACCCTGGCAAAACATCCCAATTATTAGTTGGCTCGCATTAAAAGGAAAATGCGCAAGTTGTCAAAA
TACGATAGCCGCACGCTACCCACTTGTTGAAGCCATTACCGCGCTATTGAGCTTAGCTGTTGCATATACTGTGGGTGCTA
ATGAACAAGCATTACTCTATATTTTAATTACGTGGGCATTAGTTGCATTAACATTCATTGATATTGATCACATGCTCTTA
CCCGATCAGCTTACATTACCGCTTGTGTGGCTTGCCTTAATAGCATCGGTAATGGGCTATACCATATCACCCAGCGATGC
CATTATTGGTGCGGCCTGCGGTTATTTAAGCTTATGGAGTGTATTTTGGTTATTTAAGTTACTTACCGGTAAAGAAGGTA
TGGGCTACGGCGATTTTAAATTACTTGCCGTGTTTGGTGCCCTACTTGGTTGGCAATCTCTGCTCACGATTATATTACTC
TCAAGCGTAGTCGGTGCCATTATTGGTATTACATTATTAAGTATTCAAGGAAAAGACAAAGCCACCCCTATTCCTTTTGG
CCCTTACTTGGCAATCGCAGGTTGGATCGCTATGCTTTGGGGCAACCAACTGCAAACTATGTATTTAAATTTAGTTGGTT
ACTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-130)

• Type IV leader peptidase family (Peptidase_A24) (142-250)

3D structure

• AlphaFold DB: A0A1Q2GYI3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	51.903	98.299	0.51
pilD	Vibrio campbellii strain DS40M4	51.22	97.619	0.5
pilD	Acinetobacter nosocomialis M2	50	98.639	0.493
pilD	Acinetobacter baumannii D1279779	50	98.639	0.493
pilD	Neisseria gonorrhoeae MS11	44.867	89.456	0.401