Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   B0W48_RS10475 Genome accession   NZ_CP019628
Coordinates   2360217..2361893 (-) Length   558 a.a.
NCBI ID   WP_077536897.1    Uniprot ID   -
Organism   Pseudoalteromonas aliena strain EH1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2355217..2366893
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B0W48_RS10450 (B0W48_10500) - 2355898..2356845 (-) 948 WP_077536892.1 alpha/beta fold hydrolase -
  B0W48_RS10455 (B0W48_10505) - 2356849..2358264 (-) 1416 WP_077536893.1 ATP-binding protein -
  B0W48_RS10460 (B0W48_10510) - 2358242..2358931 (-) 690 WP_077536894.1 response regulator -
  B0W48_RS10465 (B0W48_10515) - 2359119..2359607 (+) 489 WP_077536895.1 Spy/CpxP family protein refolding chaperone -
  B0W48_RS10470 (B0W48_10520) yacG 2359680..2359910 (-) 231 WP_077536896.1 DNA gyrase inhibitor YacG -
  B0W48_RS10475 (B0W48_10525) pilB 2360217..2361893 (-) 1677 WP_077536897.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B0W48_RS10480 (B0W48_10530) coaE 2362009..2362653 (-) 645 WP_077536898.1 dephospho-CoA kinase -
  B0W48_RS10485 (B0W48_10535) pilD 2362655..2363539 (-) 885 WP_077536899.1 prepilin peptidase Machinery gene
  B0W48_RS10490 (B0W48_10540) pilC 2363556..2364782 (-) 1227 WP_077536900.1 type II secretion system F family protein Machinery gene
  B0W48_RS10495 (B0W48_10545) - 2364888..2365331 (-) 444 WP_077536901.1 pilin -
  B0W48_RS10500 (B0W48_10550) nadC 2365998..2366843 (-) 846 WP_077536902.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62094.70 Da        Isoelectric Point: 6.2150

>NTDB_id=217400 B0W48_RS10475 WP_077536897.1 2360217..2361893(-) (pilB) [Pseudoalteromonas aliena strain EH1]
MNINSPLLRKFITLGRIDADMVKEKQQEFTSTAELISKCGKIDSKDLAAQCIDLFRVPYFDLKDFDPAKIPAELVKEKLI
RKHHILPLVKKDRKVYIAASDPTDYGAFENFEFSTGLSCEIVVVDYIQLNNKIEQLLDAAGSLNLSDDEFKEFADLDTEG
VKDSAPKDDEKEDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVASPPNTLATKLSARIKVMARLDI
AEKRKPQDGRIKLKITERKSIDFRVSSMPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYMDALAQPQGMILVTGPT
GSGKTVSLYTGLNILNKPERNISTAEDPVEINLEGINQVQINIKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKTPEILPSEELTRQGFSPQQISEMTL
YAPKGCDSCTDGYKGRVGIYEVMQITPEIAQIIMRGGNSLEIADVSLKAGFNNLRISGIRKAADGMTSLAEINRVTNF

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=217400 B0W48_RS10475 WP_077536897.1 2360217..2361893(-) (pilB) [Pseudoalteromonas aliena strain EH1]
ATGAATATTAACTCACCGTTATTGAGAAAGTTTATTACCCTTGGTCGTATCGATGCAGATATGGTCAAAGAAAAACAACA
AGAATTTACCTCAACAGCTGAGCTTATTTCTAAATGTGGCAAAATCGACAGTAAAGATTTAGCTGCACAATGCATCGATT
TATTTCGCGTACCCTACTTCGATCTAAAAGATTTTGATCCTGCTAAAATTCCTGCAGAGCTGGTAAAAGAAAAGCTTATT
CGCAAGCATCATATTTTACCGCTGGTAAAAAAAGATAGAAAAGTTTACATCGCAGCCTCTGATCCAACCGACTATGGCGC
ATTTGAAAATTTTGAATTTAGCACTGGTTTGTCGTGTGAGATTGTTGTTGTTGATTATATTCAGCTTAATAATAAAATTG
AGCAATTACTCGATGCAGCGGGAAGTTTAAATTTAAGTGATGACGAATTTAAAGAATTTGCAGACTTAGACACCGAGGGT
GTAAAAGACTCCGCACCAAAAGATGATGAAAAAGAAGATGCGCCGATCATTGTTTACATCAATAAAATTTTGATGGATGC
AATTAAAAAAGGCGCGTCTGATCTACACTTTGAGCCCTATGAACACAAATACCGCATCCGTTTTCGTATTGATGGCATTT
TGCACGAAGTAGCAAGTCCGCCCAATACTCTTGCAACTAAACTTTCTGCGCGTATAAAAGTAATGGCACGCCTCGATATT
GCTGAAAAACGTAAACCACAAGATGGCCGTATAAAACTAAAAATAACTGAACGAAAAAGTATCGACTTTCGAGTCAGTTC
GATGCCAACGCTGTGGGGCGAAAAAATAGTAATGCGTATCCTCGATTCTTCAAGCGCTATGCTAGGCATTGATGTATTAG
GTTACGAACCAGAACAAAAAAAATTATATATGGATGCACTTGCTCAGCCTCAAGGTATGATTTTAGTAACAGGGCCAACC
GGTTCTGGTAAAACGGTCTCACTTTACACTGGCTTAAACATATTAAATAAACCAGAGCGCAACATTAGCACCGCAGAAGA
TCCAGTTGAAATTAACCTTGAAGGTATTAACCAAGTACAAATTAACATTAAAGCGGATATGACCTTTGCCAATGCATTAC
GCGCCTTTTTACGTCAAGATCCAGACGTGGTAATGGTGGGTGAAATACGTGATTTAGAAACCGCCGAAATATCTATTAAA
GCAGCACAAACCGGCCACTTAGTATTATCAACCCTACATACTAATTCAGCACCGGAAACAATCACCCGTTTATTAAATAT
GGGTGTGCCAGCTTATAACGTAGCAAGCTCGATCAGCTTAATTATAGCCCAACGCCTAGCACGTCGTTTATGCCCTAAGT
GTAAAACGCCTGAAATACTACCAAGCGAAGAACTCACTCGCCAAGGCTTTAGCCCACAACAAATAAGTGAAATGACACTT
TATGCACCAAAAGGGTGTGATAGCTGCACTGATGGCTATAAAGGTCGTGTGGGAATTTACGAAGTTATGCAAATCACTCC
TGAAATTGCCCAAATTATTATGCGCGGCGGCAACTCATTAGAAATAGCAGATGTATCTCTTAAAGCTGGGTTTAATAATT
TGCGTATATCTGGTATTCGTAAAGCCGCTGATGGTATGACCTCACTTGCTGAAATTAACCGTGTGACTAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)

Type II/IV secretion system protein (T2SSE)

(182-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.221

92.652

0.539
  pilB Acinetobacter baylyi ADP1

58.008

91.756

0.532
  pilB Legionella pneumophila strain ERS1305867

53.497

94.803

0.507
  pilB Vibrio cholerae strain A1552

52.23

96.416

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

49.378

100

0.498
  pilB Vibrio campbellii strain DS40M4

51.028

95.878

0.489
  pilF Neisseria gonorrhoeae MS11

49.808

93.548

0.466
  pilB/pilB1 Synechocystis sp. PCC 6803

36.334

100

0.405
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.783

94.265

0.366

Multiple sequence alignment