Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   BWP33_RS00670 Genome accession   NZ_CP019448
Coordinates   134557..135831 (+) Length   424 a.a.
NCBI ID   WP_002641291.1    Uniprot ID   V9H9Q9
Organism   Simonsiella muelleri ATCC 29453     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 129557..140831
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BWP33_RS00645 (BWP33_00650) pssA 129817..130599 (+) 783 WP_169315041.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  BWP33_RS00650 (BWP33_00655) - 130807..131298 (+) 492 WP_040628648.1 hypothetical protein -
  BWP33_RS00655 (BWP33_00660) greA 131502..131978 (+) 477 WP_002641294.1 transcription elongation factor GreA -
  BWP33_RS00660 (BWP33_00665) - 132110..132553 (+) 444 WP_002641293.1 DUF4149 domain-containing protein -
  BWP33_RS00665 (BWP33_00670) pilF 132769..134457 (+) 1689 WP_002641292.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BWP33_RS00670 (BWP33_00675) pilG 134557..135831 (+) 1275 WP_002641291.1 type II secretion system F family protein Machinery gene
  BWP33_RS00675 (BWP33_00680) pilD 135833..136735 (+) 903 WP_002641290.1 A24 family peptidase Machinery gene
  BWP33_RS00680 (BWP33_00685) coaE 136738..137343 (+) 606 WP_002641289.1 dephospho-CoA kinase -
  BWP33_RS00685 (BWP33_00690) zapD 137357..138106 (+) 750 WP_002641288.1 cell division protein ZapD -
  BWP33_RS00690 (BWP33_00695) - 138152..138769 (-) 618 WP_002641287.1 HAD family hydrolase -
  BWP33_RS00695 (BWP33_00700) - 138846..139055 (-) 210 WP_211206384.1 glycine zipper 2TM domain-containing protein -
  BWP33_RS00700 (BWP33_00705) - 139170..140582 (-) 1413 WP_002641285.1 dihydrolipoyl dehydrogenase -

Sequence

Protein


Download         Length: 424 a.a.        Molecular weight: 46034.61 Da        Isoelectric Point: 10.1099

>NTDB_id=215854 BWP33_RS00670 WP_002641291.1 134557..135831(+) (pilG) [Simonsiella muelleri ATCC 29453]
MATLKKPVKKAPVKAKRPAAKPQKKERGNRYQFEGRNLQTESIVRGEVVARNEEEARQKLQRRQVKVLQIVKMKKAKNKK
ITSADIAVFTRQLCTMMKAGLPLMQAFDIVAKGHSNASMTNLLMSVRADVEQGTSMADAFSKHPKYFSKFYCNLVGAGEA
GGVLDSLLDKLATYMEKTEAIKKKVKSALTYPIAILVVAVVLVIVMMMFVLPEFKKVYDSMGAKLPPLTEFMMGISNAFV
AHGALIIIGLVGTVAGGIFIVKNSPALQKKLDALLLKLPIFGDIVMKATIARWSRTTATLFTAGVPLVEVLDSVAGASGN
IIYEEATNEIRSKVNQGISLTSGMQAAQIFPNMVVQMAAIGEEAGALDDMLNKVAEFYEEEVDTAVETLSSLMEPIIMVV
LGGIVGTILVAMYLPLFNLGQVVG

Nucleotide


Download         Length: 1275 bp        

>NTDB_id=215854 BWP33_RS00670 WP_002641291.1 134557..135831(+) (pilG) [Simonsiella muelleri ATCC 29453]
ATGGCTACTCTAAAAAAACCTGTCAAAAAAGCACCCGTGAAAGCAAAAAGACCTGCTGCCAAACCACAAAAAAAAGAGCG
TGGTAATCGATATCAATTTGAAGGGCGAAATCTTCAAACGGAGAGTATTGTGCGCGGTGAGGTGGTTGCGCGTAATGAAG
AAGAAGCACGTCAGAAATTACAACGCCGTCAGGTAAAAGTCCTGCAAATTGTTAAAATGAAAAAGGCGAAAAATAAGAAA
ATTACTTCTGCTGATATTGCCGTTTTCACGCGCCAATTATGTACCATGATGAAGGCTGGTTTGCCATTAATGCAAGCTTT
TGATATTGTGGCAAAAGGGCATTCCAATGCGTCCATGACTAATTTGCTGATGTCGGTGCGTGCAGATGTGGAACAGGGCA
CGTCTATGGCAGACGCATTTTCTAAACACCCGAAATATTTCAGTAAATTTTATTGTAACCTAGTGGGTGCAGGTGAAGCG
GGTGGTGTATTGGATTCTTTGTTAGACAAATTAGCCACTTACATGGAAAAAACCGAAGCAATTAAGAAAAAAGTAAAATC
TGCTTTAACTTATCCAATTGCCATTTTGGTGGTGGCGGTGGTATTGGTTATTGTGATGATGATGTTCGTATTGCCAGAAT
TTAAAAAGGTATACGATAGCATGGGCGCAAAATTGCCACCATTGACTGAGTTTATGATGGGTATTTCCAATGCGTTTGTG
GCTCATGGTGCATTGATTATTATTGGTTTAGTAGGTACGGTTGCTGGTGGGATTTTTATTGTGAAAAACTCGCCTGCATT
ACAGAAGAAATTAGACGCTTTATTACTGAAATTGCCTATTTTTGGTGATATTGTGATGAAAGCAACCATTGCACGTTGGT
CGCGTACTACGGCCACGCTGTTTACGGCTGGCGTACCACTGGTGGAAGTATTGGATTCAGTGGCTGGTGCATCGGGTAAT
ATTATTTATGAAGAAGCGACTAATGAAATTCGTTCTAAAGTTAATCAGGGTATTTCTTTGACTTCAGGCATGCAGGCCGC
CCAAATTTTCCCCAATATGGTGGTGCAAATGGCGGCCATTGGTGAAGAGGCTGGTGCGCTTGATGATATGTTGAATAAAG
TGGCTGAATTCTATGAAGAAGAAGTAGATACAGCAGTGGAAACGTTGTCATCATTGATGGAACCCATCATCATGGTGGTA
TTGGGGGGTATTGTAGGGACTATTTTAGTGGCGATGTATCTGCCATTGTTCAACTTGGGTCAAGTGGTTGGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(89-212)

Type II secretion system (T2SS), protein F (T2SSF)

(293-415)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V9H9Q9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

63

94.34

0.594
  pilG Neisseria meningitidis 44/76-A

62

94.34

0.585
  pilC Legionella pneumophila strain ERS1305867

48.492

93.868

0.455
  pilC Pseudomonas stutzeri DSM 10701

44.691

95.519

0.427
  pilC Acinetobacter baumannii D1279779

45.736

91.274

0.417
  pilC Acinetobacter baylyi ADP1

45.478

91.274

0.415
  pilC Vibrio cholerae strain A1552

38.903

94.575

0.368
  pilC Vibrio campbellii strain DS40M4

37.317

96.698

0.361

Multiple sequence alignment