Detailed information NTG0215855

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: BWP33_RS00675
• Genome accession: NZ_CP019448
• Coordinates: 135833..136735 (+)
• Length: 300 a.a.
• NCBI ID: WP_002641290.1
• Uniprot ID: V9HMH3
• Organism: Simonsiella muelleri ATCC 29453
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 130833..141735

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BWP33_RS00655 (BWP33_00660)	greA	131502..131978 (+)	477	WP_002641294.1	transcription elongation factor GreA	-
BWP33_RS00660 (BWP33_00665)	-	132110..132553 (+)	444	WP_002641293.1	DUF4149 domain-containing protein	-
BWP33_RS00665 (BWP33_00670)	pilF	132769..134457 (+)	1689	WP_002641292.1	type IV-A pilus assembly ATPase PilB	Machinery gene
BWP33_RS00670 (BWP33_00675)	pilG	134557..135831 (+)	1275	WP_002641291.1	type II secretion system F family protein	Machinery gene
BWP33_RS00675 (BWP33_00680)	pilD	135833..136735 (+)	903	WP_002641290.1	A24 family peptidase	Machinery gene
BWP33_RS00680 (BWP33_00685)	coaE	136738..137343 (+)	606	WP_002641289.1	dephospho-CoA kinase	-
BWP33_RS00685 (BWP33_00690)	zapD	137357..138106 (+)	750	WP_002641288.1	cell division protein ZapD	-
BWP33_RS00690 (BWP33_00695)	-	138152..138769 (-)	618	WP_002641287.1	HAD family hydrolase	-
BWP33_RS00695 (BWP33_00700)	-	138846..139055 (-)	210	WP_211206384.1	glycine zipper 2TM domain-containing protein	-
BWP33_RS00700 (BWP33_00705)	-	139170..140582 (-)	1413	WP_002641285.1	dihydrolipoyl dehydrogenase	-
BWP33_RS00705 (BWP33_00710)	-	140890..141639 (-)	750	WP_002641284.1	glutathione peroxidase	-

Sequence

Protein

• Length: 300 a.a.
• Molecular weight: 33636.15 Da
• Isoelectric Point: 8.9966

>NTDB_id=215855 BWP33_RS00675 WP_002641290.1 135833..136735(+) (pilD) [Simonsiella muelleri ATCC 29453]
MENMAIFMAVLFGLLVGSFLNVVIYRLPVMLNREWNEAAREQLKLDDEFIQTLPENIAQTLRQRIGLTPEQMGVFTLSRP
RSRCGNCGAPVRAWQNIPIISWLILRGKCHTCQTPISIRYPLVELLTGVLFGLVAWRYGWTEITVWGCLLTAMVIAMTFI
DADTQILPDDLTKPLVWLGLLFNWRMGFVSWQQALLGAVIGYMSLYLLNKIHVILRGIDGMGQGDFKLLAAIGAWVGVAN
LPIVVLMASVIGIVAALVKRVTKSQPMAFGPCLAIAGWFIFLFHQQATMAITWWLHKSGF

Nucleotide

• Length: 903 bp

>NTDB_id=215855 BWP33_RS00675 WP_002641290.1 135833..136735(+) (pilD) [Simonsiella muelleri ATCC 29453]
ATGGAGAATATGGCGATTTTTATGGCGGTATTGTTTGGGTTATTGGTTGGCAGCTTCCTGAACGTGGTGATTTACCGTCT
GCCCGTGATGCTCAATCGAGAGTGGAACGAGGCAGCGCGTGAACAACTTAAATTAGACGATGAATTTATCCAAACGCTGC
CTGAAAATATTGCCCAAACACTTCGCCAACGTATCGGCTTGACTCCTGAACAAATGGGCGTGTTTACCTTGTCGCGTCCA
CGCTCACGCTGTGGAAATTGTGGTGCACCTGTGCGAGCGTGGCAGAATATTCCAATTATTAGTTGGTTGATTTTGCGTGG
AAAATGTCATACTTGTCAAACGCCAATCAGTATTCGTTATCCTTTGGTAGAACTGCTGACGGGGGTGTTGTTTGGTTTAG
TGGCGTGGCGATACGGTTGGACGGAAATCACCGTGTGGGGCTGTTTACTCACAGCGATGGTTATTGCAATGACATTTATT
GACGCGGACACGCAAATTTTGCCTGATGATTTGACTAAACCTTTGGTTTGGTTGGGTTTGTTATTTAATTGGCGTATGGG
CTTTGTGTCGTGGCAACAAGCATTGTTGGGTGCTGTAATTGGTTATATGAGTTTGTATTTATTGAATAAAATTCATGTAA
TTTTGCGTGGCATAGATGGTATGGGGCAGGGCGATTTTAAATTATTGGCTGCGATTGGTGCGTGGGTTGGGGTGGCAAAC
TTGCCTATTGTGGTGTTGATGGCATCGGTTATTGGCATTGTGGCGGCGTTGGTGAAACGTGTTACCAAAAGTCAGCCTAT
GGCATTTGGTCCATGTTTGGCGATTGCTGGTTGGTTTATTTTTCTTTTTCATCAACAAGCTACAATGGCAATTACGTGGT
GGTTGCATAAATCTGGATTTTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (149-256)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (11-138)

3D structure

• AlphaFold DB: V9HMH3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Neisseria gonorrhoeae MS11	53.559	98.333	0.527
pilD	Vibrio cholerae strain A1552	45.263	95	0.43
pilD	Acinetobacter nosocomialis M2	43.86	95	0.417
pilD	Vibrio campbellii strain DS40M4	43.357	95.333	0.413
pilD	Acinetobacter baumannii D1279779	43.509	95	0.413