Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   BWP33_RS00665 Genome accession   NZ_CP019448
Coordinates   132769..134457 (+) Length   562 a.a.
NCBI ID   WP_002641292.1    Uniprot ID   V9H6S8
Organism   Simonsiella muelleri ATCC 29453     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 127769..139457
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BWP33_RS00640 (BWP33_00645) efeB 128189..129457 (+) 1269 WP_002641297.1 iron uptake transporter deferrochelatase/peroxidase subunit -
  BWP33_RS00645 (BWP33_00650) pssA 129817..130599 (+) 783 WP_169315041.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  BWP33_RS00650 (BWP33_00655) - 130807..131298 (+) 492 WP_040628648.1 hypothetical protein -
  BWP33_RS00655 (BWP33_00660) greA 131502..131978 (+) 477 WP_002641294.1 transcription elongation factor GreA -
  BWP33_RS00660 (BWP33_00665) - 132110..132553 (+) 444 WP_002641293.1 DUF4149 domain-containing protein -
  BWP33_RS00665 (BWP33_00670) pilF 132769..134457 (+) 1689 WP_002641292.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BWP33_RS00670 (BWP33_00675) pilG 134557..135831 (+) 1275 WP_002641291.1 type II secretion system F family protein Machinery gene
  BWP33_RS00675 (BWP33_00680) pilD 135833..136735 (+) 903 WP_002641290.1 A24 family peptidase Machinery gene
  BWP33_RS00680 (BWP33_00685) coaE 136738..137343 (+) 606 WP_002641289.1 dephospho-CoA kinase -
  BWP33_RS00685 (BWP33_00690) zapD 137357..138106 (+) 750 WP_002641288.1 cell division protein ZapD -
  BWP33_RS00690 (BWP33_00695) - 138152..138769 (-) 618 WP_002641287.1 HAD family hydrolase -
  BWP33_RS00695 (BWP33_00700) - 138846..139055 (-) 210 WP_211206384.1 glycine zipper 2TM domain-containing protein -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62447.16 Da        Isoelectric Point: 5.2321

>NTDB_id=215853 BWP33_RS00665 WP_002641292.1 132769..134457(+) (pilF) [Simonsiella muelleri ATCC 29453]
MSIGLLRLLKQEGLIPSGKVEEKYRKALDNNKNIIPMFFDDKVIAPEDLASLVSRVFNYPQFDLRAFPARKILTDVMEEA
QIQENRCIPIFKRGKRVFVAVSDPTILQNVQKFVFNSGLSPELVIVRDDQLDSMLEQFSQSNTALIKEMGMDANLQEAQA
ETMIVSGEEEDDGPIAKFIQKVLFDAVNTGASDIHFEFYEFMARVRYRTDGVLREVVQPPLALRNNLASRIKVMAKLDIS
EKRIPQDGRIQLQFSKGSKAIDFRVSTMPCLFGEKVVMRILNSDAGFLNINQLGFEDFQKDLILEAIHRPYGMVLVTGPT
GSGKTVSLYTCLNILNTDDVNISTAEDPAEINLPGINQVNVNDKQGLTFEAALKAFLRQDPDIIMIGEIRDLGTADIAIK
AAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASAVNLIMAQRLARRLCSNCKAPMERPPEKVLREAGFTDEDLAGNW
TMYRAVGCEACKGKGYKGRAGLYEVMPMTERMKAVIMKGGTEVDIANIAYEEGLVDLRRSGLLKVMQGITTLEEVLATTN
EG

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=215853 BWP33_RS00665 WP_002641292.1 132769..134457(+) (pilF) [Simonsiella muelleri ATCC 29453]
ATGAGTATTGGTTTGCTACGATTATTGAAACAAGAAGGGCTGATTCCGTCCGGTAAAGTAGAAGAAAAGTATCGTAAGGC
ATTGGATAATAATAAAAATATTATCCCAATGTTTTTTGATGATAAAGTGATTGCACCTGAGGATTTGGCGTCATTGGTGT
CGCGCGTATTCAATTATCCACAATTTGATTTGAGAGCTTTTCCAGCACGTAAAATTCTAACCGATGTGATGGAAGAAGCG
CAAATACAAGAAAATCGCTGTATTCCGATTTTCAAACGTGGCAAAAGGGTGTTTGTGGCAGTATCCGATCCAACCATTTT
GCAAAATGTGCAAAAATTTGTGTTCAATTCCGGATTATCGCCAGAATTGGTGATTGTACGGGATGATCAGCTGGATTCTA
TGTTGGAACAATTCTCACAAAGTAACACCGCGCTGATTAAAGAAATGGGTATGGACGCGAATTTGCAGGAAGCCCAAGCT
GAAACCATGATTGTGAGCGGCGAAGAAGAAGACGATGGGCCTATTGCGAAATTTATTCAAAAAGTTTTATTTGATGCGGT
CAACACAGGTGCATCCGATATTCACTTTGAGTTTTATGAGTTTATGGCGCGTGTGCGTTATCGTACGGACGGCGTGTTAC
GTGAAGTGGTGCAACCACCTTTGGCTTTACGTAATAATTTAGCATCGCGTATTAAAGTGATGGCAAAATTAGATATTTCT
GAAAAACGCATTCCACAGGATGGACGTATTCAATTGCAGTTTTCCAAAGGTTCTAAAGCGATTGATTTTCGTGTGAGTAC
CATGCCTTGTTTGTTTGGTGAAAAAGTTGTAATGCGTATTTTGAATTCAGACGCTGGCTTTTTGAATATTAATCAATTGG
GTTTTGAAGATTTTCAAAAAGATTTGATTTTGGAAGCAATTCATCGTCCATACGGCATGGTATTGGTAACGGGGCCTACG
GGTTCGGGTAAAACCGTATCGCTTTATACTTGCTTGAATATTCTGAATACAGATGATGTGAATATTTCTACCGCCGAAGA
CCCAGCGGAGATTAACTTACCAGGTATTAATCAGGTAAACGTGAATGATAAACAAGGTTTAACATTTGAGGCAGCCTTAA
AGGCTTTCTTGCGTCAAGACCCTGATATTATTATGATTGGTGAGATTCGCGATTTGGGTACAGCAGATATTGCGATTAAA
GCCGCACAAACAGGTCATATGGTGTTCTCGACCTTGCACACGAATAATGCGCCTGCGACTTTATCGCGTATGTTGAATAT
GGGTGTTGCGCCGTTTAATATTGCTTCTGCGGTAAATTTGATTATGGCGCAACGTTTGGCGCGTCGCTTGTGTTCTAATT
GTAAAGCACCGATGGAGCGTCCGCCTGAAAAAGTTTTACGTGAAGCTGGTTTCACCGATGAAGATTTGGCAGGTAATTGG
ACGATGTATCGTGCAGTGGGTTGCGAAGCATGTAAAGGCAAAGGTTACAAAGGGCGCGCTGGTTTGTATGAAGTGATGCC
CATGACTGAACGCATGAAAGCCGTGATTATGAAAGGCGGTACGGAAGTGGATATTGCCAATATTGCTTATGAAGAAGGTT
TGGTGGATTTACGCCGTTCGGGTTTACTCAAAGTGATGCAAGGCATTACCACGTTGGAAGAAGTATTGGCAACCACGAAC
GAAGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(181-449)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V9H6S8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

66.667

99.822

0.665
  pilB Acinetobacter baylyi ADP1

51.779

100

0.518
  pilB Acinetobacter baumannii D1279779

51.423

100

0.514
  pilB Legionella pneumophila strain ERS1305867

48.214

99.644

0.48
  pilB Vibrio cholerae strain A1552

44.681

100

0.448
  pilB Vibrio parahaemolyticus RIMD 2210633

43.363

100

0.436
  pilB Vibrio campbellii strain DS40M4

43.186

100

0.434
  pilF Thermus thermophilus HB27

38.104

99.466

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.692

92.349

0.367

Multiple sequence alignment