Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PspR76_RS04770 Genome accession   NZ_CP019428
Coordinates   1078062..1079270 (-) Length   402 a.a.
NCBI ID   WP_159954188.1    Uniprot ID   -
Organism   Pseudomonas sp. R76     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1073062..1084270
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PspR76_RS04730 (PspR76_04840) - 1073957..1074130 (-) 174 WP_064450648.1 DUF3094 family protein -
  PspR76_RS04735 (PspR76_04845) - 1074220..1074849 (-) 630 WP_159954182.1 DUF1780 domain-containing protein -
  PspR76_RS04740 (PspR76_04850) - 1074950..1075417 (+) 468 WP_159954183.1 FAD/FMN-containing dehydrogenase -
  PspR76_RS04745 (PspR76_04855) - 1075414..1076103 (+) 690 WP_159954184.1 energy-coupling factor ABC transporter permease -
  PspR76_RS04750 (PspR76_04860) - 1076170..1076385 (+) 216 WP_003171680.1 hypothetical protein -
  PspR76_RS04755 (PspR76_04865) yacG 1076382..1076582 (-) 201 WP_159954185.1 DNA gyrase inhibitor YacG -
  PspR76_RS04760 (PspR76_04870) coaE 1076579..1077202 (-) 624 WP_159954186.1 dephospho-CoA kinase -
  PspR76_RS04765 (PspR76_04875) pilD 1077199..1078065 (-) 867 WP_159954187.1 A24 family peptidase Machinery gene
  PspR76_RS04770 (PspR76_04880) pilC 1078062..1079270 (-) 1209 WP_159954188.1 type II secretion system F family protein Machinery gene
  PspR76_RS04775 (PspR76_04885) - 1079474..1079887 (+) 414 WP_159961324.1 pilin -
  PspR76_RS04785 (PspR76_04895) - 1080192..1080497 (+) 306 WP_159954189.1 DUF6388 family protein -
  PspR76_RS04790 (PspR76_04900) nadC 1080758..1081606 (-) 849 WP_159954190.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PspR76_RS04795 (PspR76_04905) - 1081852..1084008 (+) 2157 WP_159954191.1 DUF1631 domain-containing protein -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43547.15 Da        Isoelectric Point: 10.1647

>NTDB_id=215685 PspR76_RS04770 WP_159954188.1 1078062..1079270(-) (pilC) [Pseudomonas sp. R76]
MNDTATIYAWEGINRKGRRVSGHTVGQNLALIKAQLRQQGICPGRVRKKTTRLPRFAPPIKPADIALFTRQLATLLKAGI
PLLQAFDIIREGFDSRPMRELVRALKQEIAAGNNLAAALRKHPRHFDDLYCNLVAAGEQAGALETLLERVAVHLEKSQLL
KARIKKAMTYPIAVIVVACVVSSVLLIHVVPQFQNLFAGVDGQLPDLTLTVIALSEFLQRAWWMLALGVAATLAGLRQAY
RAAPGFRHWLDTGLLNAPLAGTLLKKTAVARYARTLSTTFAAGVPLVQALDSVAGAVGNGLFKQAIEHMRHDVSTGMPLH
QSMASKGLFPGMAVQMTAIGEESGTLDTLLEKVANHYEADVDNLVDNLTSLMEPLIMVVLGGIVGTLVIAMYLPVFQMGS
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=215685 PspR76_RS04770 WP_159954188.1 1078062..1079270(-) (pilC) [Pseudomonas sp. R76]
ATGAATGACACTGCAACGATCTACGCCTGGGAAGGCATCAACCGCAAAGGGCGCAGGGTGTCCGGGCACACCGTCGGCCA
AAACCTGGCGCTGATCAAGGCGCAGCTACGCCAGCAAGGGATCTGCCCCGGCCGTGTGCGCAAGAAGACCACACGCCTGC
CACGCTTCGCGCCGCCGATAAAACCCGCCGATATTGCCTTGTTCACGCGCCAGTTGGCGACATTGCTGAAGGCTGGCATT
CCGTTGCTGCAAGCCTTCGACATTATCCGCGAAGGCTTCGATAGCCGCCCGATGCGCGAGCTGGTCAGGGCCTTGAAACA
GGAGATCGCCGCCGGCAACAACCTCGCCGCAGCACTGCGTAAGCACCCTCGTCACTTCGATGACCTGTATTGCAACCTGG
TGGCCGCCGGTGAGCAGGCCGGCGCGCTGGAAACCCTGCTGGAGCGGGTCGCCGTGCACTTGGAAAAAAGCCAGCTGCTC
AAGGCACGCATCAAGAAGGCCATGACCTACCCCATCGCCGTCATCGTGGTCGCCTGCGTGGTCAGCAGCGTCCTGCTGAT
TCATGTGGTGCCGCAGTTCCAGAACCTGTTTGCCGGGGTCGACGGCCAACTGCCCGACCTGACGTTGACGGTGATTGCCC
TGTCCGAATTCCTCCAGCGCGCCTGGTGGATGCTTGCGCTTGGCGTGGCCGCCACCCTGGCGGGCCTGCGCCAGGCCTAC
CGCGCAGCGCCAGGTTTTCGTCACTGGCTGGATACCGGTTTGTTGAACGCGCCGCTGGCAGGCACACTGCTGAAAAAAAC
CGCCGTGGCTCGCTATGCCCGCACACTCTCGACCACCTTTGCCGCGGGCGTGCCCTTGGTGCAGGCGTTGGATTCCGTGG
CAGGCGCGGTGGGTAATGGGTTGTTCAAACAGGCGATCGAGCATATGCGTCACGATGTATCCACAGGCATGCCGTTGCAT
CAATCCATGGCCAGCAAGGGGCTGTTTCCCGGTATGGCGGTCCAGATGACGGCGATTGGCGAAGAATCCGGCACCCTCGA
CACCCTGCTGGAAAAAGTCGCCAATCACTACGAGGCCGACGTGGACAACCTGGTCGACAACCTCACCAGCCTGATGGAGC
CGCTGATCATGGTGGTACTGGGTGGCATTGTCGGCACCTTGGTCATCGCCATGTACTTGCCGGTATTCCAAATGGGGTCG
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

59.241

98.259

0.582
  pilC Acinetobacter baylyi ADP1

51.263

98.507

0.505
  pilC Acinetobacter baumannii D1279779

50.38

98.259

0.495
  pilC Legionella pneumophila strain ERS1305867

47.103

98.756

0.465
  pilC Thermus thermophilus HB27

38.404

99.751

0.383
  pilC Vibrio cholerae strain A1552

38.579

98.01

0.378
  pilG Neisseria gonorrhoeae MS11

41.033

91.542

0.376
  pilG Neisseria meningitidis 44/76-A

40.217

91.542

0.368
  pilC Vibrio campbellii strain DS40M4

37.31

98.01

0.366

Multiple sequence alignment