Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   BWZ32_RS15480 Genome accession   NZ_CP019320
Coordinates   3091053..3092279 (+) Length   408 a.a.
NCBI ID   WP_101957317.1    Uniprot ID   -
Organism   Vibrio vulnificus strain VV2014DJH     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3086053..3097279
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BWZ32_RS15460 (BWZ32_15345) ampD 3087092..3087664 (-) 573 WP_045622095.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BWZ32_RS15465 (BWZ32_15350) nadC 3087757..3088644 (+) 888 WP_061058907.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BWZ32_RS15470 (BWZ32_15355) - 3088864..3089316 (+) 453 WP_060530791.1 pilin -
  BWZ32_RS15475 (BWZ32_15360) pilB 3089321..3091006 (+) 1686 WP_101957316.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BWZ32_RS15480 (BWZ32_15365) pilC 3091053..3092279 (+) 1227 WP_101957317.1 type II secretion system F family protein Machinery gene
  BWZ32_RS15485 (BWZ32_15370) pilD 3092373..3093242 (+) 870 WP_101957318.1 prepilin peptidase Machinery gene
  BWZ32_RS15490 (BWZ32_15375) coaE 3093244..3093852 (+) 609 WP_045596843.1 dephospho-CoA kinase -
  BWZ32_RS15495 (BWZ32_15380) zapD 3093885..3094625 (+) 741 WP_045596844.1 cell division protein ZapD -
  BWZ32_RS15500 (BWZ32_15385) yacG 3094688..3094882 (+) 195 WP_026060801.1 DNA gyrase inhibitor YacG -
  BWZ32_RS15505 (BWZ32_15390) rplS 3095165..3095518 (-) 354 WP_011079544.1 50S ribosomal protein L19 -
  BWZ32_RS15510 (BWZ32_15395) trmD 3095560..3096309 (-) 750 WP_101957319.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  BWZ32_RS15515 (BWZ32_15400) rimM 3096337..3096885 (-) 549 WP_011079542.1 ribosome maturation factor RimM -
  BWZ32_RS15520 (BWZ32_15405) rpsP 3096901..3097149 (-) 249 WP_011079541.1 30S ribosomal protein S16 -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45241.54 Da        Isoelectric Point: 10.3244

>NTDB_id=214839 BWZ32_RS15480 WP_101957317.1 3091053..3092279(+) (pilC) [Vibrio vulnificus strain VV2014DJH]
MKLKTHSQIKNYQWKGINGAGKKVSGNTLALTELEVRDKLKMQHIQIKKIKKSSVSVFTRLSHKVKGKDITLLTRQLATM
LTTGVPILQSLKLVADNHRKAEMKSILANVSKAVEAGTPLSKALRSASPQFDGIYVDLVATGEQSGNLDQVFERLAFYRE
KNEQLRAKVIKALIYPCMVVLVALGVSYLMLTQVIPEFRKMFTGFGAELPWFTQKVLDLSDFVQAYSSWFFITLFSAIYL
LRGLSKKSPAFRLKMSRLSLKIPVIGGVLSKAAIAKFSRTLATSFSAGIPILTSLKTTSKTAGNLHYQYAIEDVYRDTAA
GMPMYIAMRNSLAFPELVLQMVMIGEESGQMDDMLNKIATIYEFEVDNTVDNLGKILEPLIIVFLGVVVGGLVAAMYLPI
FNLMSVMG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=214839 BWZ32_RS15480 WP_101957317.1 3091053..3092279(+) (pilC) [Vibrio vulnificus strain VV2014DJH]
ATGAAGCTTAAAACCCATTCACAAATCAAAAACTATCAATGGAAAGGGATTAACGGAGCAGGAAAAAAAGTCTCCGGCAA
CACGCTGGCGCTTACCGAGCTTGAGGTGCGTGATAAGCTGAAAATGCAGCATATCCAGATCAAAAAGATCAAGAAATCCA
GTGTGTCAGTCTTCACAAGGTTAAGCCATAAAGTCAAAGGTAAAGACATCACACTGCTTACCCGCCAACTCGCCACCATG
CTGACCACTGGTGTCCCTATTCTCCAATCACTCAAATTGGTTGCTGACAACCATCGCAAAGCCGAGATGAAGTCGATTTT
AGCCAATGTGAGCAAAGCCGTTGAAGCAGGCACGCCGTTATCCAAAGCGCTTCGCAGTGCCAGCCCACAATTCGATGGCA
TTTATGTTGACTTAGTCGCCACTGGTGAACAATCGGGTAATCTTGATCAAGTTTTTGAACGCCTCGCTTTTTATCGGGAA
AAAAACGAGCAGTTGCGAGCAAAAGTGATCAAGGCGCTGATTTATCCCTGCATGGTCGTGCTGGTCGCGTTGGGGGTATC
TTATTTAATGCTGACTCAGGTTATTCCCGAGTTTCGTAAAATGTTCACCGGATTCGGCGCAGAGCTGCCTTGGTTTACGC
AAAAAGTCTTAGACCTGTCTGATTTTGTGCAAGCCTACAGCAGTTGGTTTTTCATTACGTTGTTCAGTGCCATTTATTTA
CTTCGCGGTCTAAGCAAAAAATCACCCGCATTTCGCTTAAAAATGTCACGCTTGAGTTTAAAGATCCCTGTGATTGGAGG
GGTCTTATCCAAAGCAGCGATTGCCAAATTTAGTCGCACATTGGCCACCAGTTTCAGCGCAGGTATCCCGATCCTCACCA
GTTTAAAAACCACCAGTAAAACCGCTGGCAATCTGCATTACCAATATGCGATTGAAGATGTTTATCGTGACACCGCAGCC
GGGATGCCAATGTACATCGCCATGCGCAACAGCCTTGCTTTCCCAGAATTGGTGCTGCAAATGGTGATGATTGGTGAAGA
ATCTGGCCAAATGGACGATATGCTCAACAAAATTGCCACCATCTATGAGTTTGAGGTGGATAACACCGTCGACAACCTCG
GTAAGATTCTCGAGCCGTTGATCATCGTCTTTCTGGGCGTTGTCGTCGGCGGATTAGTCGCCGCCATGTACCTGCCGATC
TTTAACTTAATGAGTGTTATGGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

78.25

98.039

0.767
  pilC Vibrio campbellii strain DS40M4

74.314

98.284

0.73
  pilC Pseudomonas stutzeri DSM 10701

41.337

99.02

0.409
  pilG Neisseria gonorrhoeae MS11

41.045

98.529

0.404
  pilG Neisseria meningitidis 44/76-A

40.796

98.529

0.402
  pilC Acinetobacter baumannii D1279779

39.756

100

0.4
  pilC Acinetobacter baylyi ADP1

40.602

97.794

0.397
  pilC Legionella pneumophila strain ERS1305867

40.299

98.529

0.397

Multiple sequence alignment