Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BWZ32_RS15475 Genome accession   NZ_CP019320
Coordinates   3089321..3091006 (+) Length   561 a.a.
NCBI ID   WP_101957316.1    Uniprot ID   -
Organism   Vibrio vulnificus strain VV2014DJH     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3084321..3096006
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BWZ32_RS15455 (BWZ32_15340) pdhR 3085936..3086703 (-) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  BWZ32_RS15460 (BWZ32_15345) ampD 3087092..3087664 (-) 573 WP_045622095.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BWZ32_RS15465 (BWZ32_15350) nadC 3087757..3088644 (+) 888 WP_061058907.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BWZ32_RS15470 (BWZ32_15355) - 3088864..3089316 (+) 453 WP_060530791.1 pilin -
  BWZ32_RS15475 (BWZ32_15360) pilB 3089321..3091006 (+) 1686 WP_101957316.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BWZ32_RS15480 (BWZ32_15365) pilC 3091053..3092279 (+) 1227 WP_101957317.1 type II secretion system F family protein Machinery gene
  BWZ32_RS15485 (BWZ32_15370) pilD 3092373..3093242 (+) 870 WP_101957318.1 prepilin peptidase Machinery gene
  BWZ32_RS15490 (BWZ32_15375) coaE 3093244..3093852 (+) 609 WP_045596843.1 dephospho-CoA kinase -
  BWZ32_RS15495 (BWZ32_15380) zapD 3093885..3094625 (+) 741 WP_045596844.1 cell division protein ZapD -
  BWZ32_RS15500 (BWZ32_15385) yacG 3094688..3094882 (+) 195 WP_026060801.1 DNA gyrase inhibitor YacG -
  BWZ32_RS15505 (BWZ32_15390) rplS 3095165..3095518 (-) 354 WP_011079544.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62310.20 Da        Isoelectric Point: 5.1037

>NTDB_id=214838 BWZ32_RS15475 WP_101957316.1 3089321..3091006(+) (pilB) [Vibrio vulnificus strain VV2014DJH]
MTNLATILRQANQLSLTQEQACRETIQASGVTAPEALLQLGFFQPDELTEQLSAIFGLPCTDLNQYDYQELCNQLGLREL
ITRHQALPLQKSNSGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQELQAAIRRLYGKSIKQSQSSGLKEIHQDELAN
LVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGMI
LMTGPTGSGKTVSLYTGLSLLNQPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADDL
IYQADRQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLAQQKDMMTLKDSGLEKLKQGITSLEELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=214838 BWZ32_RS15475 WP_101957316.1 3089321..3091006(+) (pilB) [Vibrio vulnificus strain VV2014DJH]
ATGACTAACCTTGCCACAATTCTTCGTCAGGCCAATCAACTTAGCCTAACGCAAGAACAAGCGTGTCGAGAGACAATACA
AGCTTCCGGTGTCACAGCACCGGAAGCTTTGTTGCAACTGGGATTTTTCCAGCCGGATGAGTTAACCGAGCAGCTAAGCG
CGATTTTTGGCTTACCATGTACTGACCTCAACCAATATGACTACCAAGAGCTGTGTAATCAACTTGGGTTACGTGAGCTG
ATCACTCGTCACCAAGCACTGCCACTGCAAAAAAGCAACTCCGGTTTAATACTCGCGGTTGCAGACCCTACCAATATTCA
AGCTGAAGATGATTTCCGTTTTGCCACAGGTGTGCAGGTGGAGATTGTTTTGGCCGATTGCCAAGAGCTGCAAGCGGCAA
TTCGTAGACTCTATGGAAAGAGCATCAAACAGAGCCAAAGCTCTGGTTTAAAAGAGATTCACCAAGATGAGTTGGCGAAT
TTGGTTGAGGTTTCAGATGACGAAGTCGAGTCAATTGAAGACTTAAGCCAAGATGAATCCCCCGTCAGTCGCTTTATCAA
CCAAATCCTGCTGGATGCGGTGCGCAAAGGCGCATCTGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTGCGAT
TGCGTTGCGATGGTATTCTGGTGGAAGTGCAGCAGCCTCCAAGCCATTTAAGCCGCCGTTTATCCGCGAGGATCAAGATC
CTTTCCAAGTTGGATATTGCGGAAAGGCGTCTGCCACAAGATGGACGGATTAAGCTGCGCCTCAACCAAGAAACCGCGAT
TGATATGCGCGTTTCCACCTTACCCACACTGTTTGGTGAAAAAATTGTGTTGCGACTTCTCGACAGCTCGAGCAGTAGCT
TGGATATTGATAAGTTAGGCTACAACCCGCAACAAAAGCAGCTCTACCTCGATGCCCTGCGCAAACCGCAAGGGATGATT
TTGATGACAGGCCCAACAGGAAGCGGTAAAACCGTTTCTCTGTACACAGGCTTGAGTTTGCTTAACCAGCCAGAAGTGAA
TATCTCCACCGCGGAAGACCCGGTCGAAATTAACCTCCCAGGTATCAACCAAGTTCAAGTGAAACCCAAAATTGGTTTTG
GTTTTGCAGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCAGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAAACC
GCCGAGATAGCAGTCAAAGCAGCCCAAACCGGCCACCTTGTGCTATCCACCTTGCATACCAACTCGGCTGCTGAAACCAT
TGTTCGTCTATCTAACATGGGGATCGCCAGCTTTAACTTGGCTTCATCCTTGAGTTTGATCATTGCTCAGCGTCTGGCTC
GTCGGTTATGCAGCCATTGCAAGCAACCTCATCCATACAACGCCACATTTGAGTTACTCGGCCTAAAAGCTGACGACTTG
ATTTATCAAGCTGATAGACAGGGTTGCCATCATTGTACTCATGGTTATTCCGGTCGTGTCGGTATATACGAAGTCATGCC
TTTTGATGAGCAGCTTGCAGAGGCGATCGTTAAAGGCGCCTCGGTACAAAGCTTGGAGATGCTTGCTCAGCAAAAAGACA
TGATGACACTCAAAGATTCCGGCCTTGAAAAACTCAAACAGGGCATCACCAGCTTAGAAGAGCTGCAACGCGTCCTCTAT
TTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

76.827

100

0.768
  pilB Vibrio parahaemolyticus RIMD 2210633

75.893

99.822

0.758
  pilB Vibrio cholerae strain A1552

74.377

100

0.745
  pilB Legionella pneumophila strain ERS1305867

52.827

91.444

0.483
  pilB Acinetobacter baumannii D1279779

51.538

92.692

0.478
  pilB Acinetobacter baylyi ADP1

50.287

93.226

0.469
  pilF Neisseria gonorrhoeae MS11

45.115

100

0.453
  pilF Thermus thermophilus HB27

40.429

99.643

0.403
  pilB/pilB1 Synechocystis sp. PCC 6803

36.713

100

0.374
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.302

94.474

0.362
  pilB Haemophilus influenzae Rd KW20

41.942

86.275

0.362

Multiple sequence alignment