Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   FORC54_RS14785 Genome accession   NZ_CP019121
Coordinates   2928232..2930016 (-) Length   594 a.a.
NCBI ID   WP_017419660.1    Uniprot ID   A0A087IG05
Organism   Vibrio vulnificus strain FORC_054     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2923232..2935016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC54_RS14765 (FORC54_2658) - 2923934..2924761 (-) 828 WP_017419662.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  FORC54_RS14770 (FORC54_2659) - 2924841..2926337 (-) 1497 WP_017419661.1 AAA family ATPase -
  FORC54_RS14775 (FORC54_2660) aroB 2926388..2927479 (-) 1092 WP_011079358.1 3-dehydroquinate synthase -
  FORC54_RS14780 (FORC54_2661) aroK 2927512..2928030 (-) 519 WP_011079357.1 shikimate kinase AroK -
  FORC54_RS14785 (FORC54_2662) pilQ 2928232..2930016 (-) 1785 WP_017419660.1 type IV pilus secretin PilQ Machinery gene
  FORC54_RS14790 (FORC54_2663) pilP 2930030..2930542 (-) 513 WP_101958982.1 pilus assembly protein PilP Machinery gene
  FORC54_RS14795 (FORC54_2664) pilO 2930532..2931122 (-) 591 WP_101958983.1 type 4a pilus biogenesis protein PilO Machinery gene
  FORC54_RS14800 (FORC54_2665) pilN 2931115..2931705 (-) 591 WP_045609935.1 PilN domain-containing protein Machinery gene
  FORC54_RS14805 (FORC54_2666) pilM 2931695..2932702 (-) 1008 WP_017419656.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 594 a.a.        Molecular weight: 64591.66 Da        Isoelectric Point: 4.5077

>NTDB_id=213346 FORC54_RS14785 WP_017419660.1 2928232..2930016(-) (pilQ) [Vibrio vulnificus strain FORC_054]
MICELMMKNKIQLFPGMLSQFVCLLSVCIFTTFANAQSEAENALKSIDFRTNAAKDAIIIVELATPATVVDLKPVAEGLS
IELINTSVKDEQLYLLDVKDFSTVVESIEVFREVSKTRLVANVSGEFRHDYRLSGRYLEVTISRIKSEQEAREQSVLEKE
GKLISINFQDIPVRNVLQLIADYNGFNLVVSDSVTGNVTLRLDGVPWQQVLDIILQVKGLDKRVEGNVVLIAPQDELDLR
EKQQLEKERLAEELGSLKSEIIKVNFAKASDIAEMINGKESVSMLSERGSVAIDERTNSLLLRELPENIEVIREIINSLD
IPVKQVQIEARIVTVNEGNLDELGVRWGFTSINGSTTVGGSIENNLATIGLYNAGSGSGSGDSGSGGSGDEGGSNSIDDF
LNVNLAATSANASSIAFQVAKLGSDLLLDLELSALQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVSF
KKAVLSLKVTPQITPDNRLVLDLSVTQDRPGEIVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNTTDKVPL
LGDLPLLGALFRRSYENVGKRELLIFVTPKVILQ

Nucleotide


Download         Length: 1785 bp        

>NTDB_id=213346 FORC54_RS14785 WP_017419660.1 2928232..2930016(-) (pilQ) [Vibrio vulnificus strain FORC_054]
GTGATTTGTGAGTTGATGATGAAAAACAAAATCCAATTGTTTCCGGGAATGCTTTCCCAATTCGTCTGCCTGTTGTCTGT
TTGCATCTTTACCACTTTTGCAAACGCACAATCTGAAGCAGAAAATGCGCTCAAGAGCATCGATTTCAGAACCAATGCAG
CCAAAGATGCGATTATCATCGTTGAATTGGCAACGCCTGCAACCGTGGTGGATTTGAAACCAGTGGCAGAAGGATTGAGC
ATCGAGTTGATCAATACCTCGGTTAAAGATGAGCAGCTCTATCTGCTCGATGTCAAAGATTTTTCGACCGTGGTTGAAAG
TATCGAAGTATTCAGAGAAGTGTCCAAAACCCGTCTTGTTGCGAATGTCAGCGGTGAATTTCGTCACGACTATCGTCTTT
CTGGACGCTATCTCGAGGTGACAATTTCTCGCATCAAATCGGAGCAAGAAGCCCGTGAACAAAGTGTGTTGGAAAAAGAA
GGCAAGCTGATTTCTATCAACTTCCAAGATATTCCAGTGCGTAACGTTTTGCAGCTTATCGCAGACTACAATGGCTTCAA
CCTCGTGGTGTCTGATTCTGTCACTGGTAATGTGACGCTGAGATTGGATGGGGTACCTTGGCAGCAAGTGCTCGACATCA
TTCTTCAAGTGAAAGGTCTAGACAAACGTGTTGAAGGCAATGTTGTGCTCATTGCTCCTCAAGATGAACTGGATTTGCGA
GAGAAGCAGCAGCTTGAGAAAGAACGTCTGGCGGAAGAGCTTGGCTCCCTTAAATCGGAAATCATCAAAGTCAACTTTGC
TAAAGCGTCAGACATTGCTGAGATGATCAATGGCAAAGAGTCGGTCAGCATGCTGTCAGAACGTGGTAGTGTGGCGATTG
ATGAGCGGACAAACTCTCTTTTGTTGCGTGAGTTACCCGAAAACATCGAAGTGATTCGAGAAATTATCAATTCGTTGGAT
ATCCCAGTCAAACAGGTACAAATTGAAGCACGCATTGTCACGGTGAATGAAGGCAATCTTGATGAGCTTGGTGTCCGTTG
GGGGTTCACCTCCATCAACGGCAGCACAACCGTCGGCGGTTCTATCGAAAACAACCTAGCAACCATTGGTCTCTACAATG
CAGGCAGCGGGAGCGGCAGTGGTGACTCAGGTAGCGGCGGCAGTGGTGATGAAGGCGGGTCGAACTCCATTGATGATTTC
CTCAATGTCAATCTTGCTGCGACTTCTGCAAACGCCTCAAGTATTGCTTTCCAAGTGGCTAAATTGGGGTCGGATTTGTT
GTTGGATCTCGAGCTATCAGCACTGCAGCGCGAATCAAAAGCGGAAATTATTTCTAGCCCTCGTTTGATCACAACCAATA
AGAAGCCTGCTTATATCGAGCAAGGTACAGAGATTCCTTATCTAGAGTCTTCCTCCAGTGGAGCGACCACGGTTTCCTTT
AAGAAAGCGGTGCTTAGCCTGAAAGTAACCCCTCAGATCACGCCTGACAATCGTCTCGTGTTGGATTTAAGCGTTACACA
AGATAGACCTGGCGAAATTGTCAAAACAGGCACTGGGGAAGCGGTTGCGATTGATACTCAGCGCATCGGTACACAAGTCT
TGGTCAATAATGGTGAAACGGTAGTGTTAGGCGGTATCTTCCAACACAGCATAACCAATACCACGGACAAGGTTCCTCTG
TTAGGCGATTTACCCTTATTGGGTGCGCTTTTCCGCCGTAGCTATGAAAACGTAGGCAAACGTGAATTGCTGATATTTGT
CACACCTAAGGTTATCTTGCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(260-325)

Bacterial type II and III secretion system protein (Secretin)

(434-592)

Secretin and TonB N terminus short domain (STN)

(186-234)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A087IG05

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

77.966

99.327

0.774
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

74.068

99.327

0.736
  pilQ Vibrio cholerae strain A1552

74.068

99.327

0.736
  pilQ Legionella pneumophila strain Lp02

41.429

94.276

0.391
  pilQ Legionella pneumophila strain ERS1305867

41.429

94.276

0.391
  pilQ Pseudomonas aeruginosa PAK

38.435

96.801

0.372
  comQ Acinetobacter baylyi ADP1

36.824

99.663

0.367

Multiple sequence alignment