Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PFAS1_RS15695 Genome accession   NZ_CP018319
Coordinates   3380903..3382120 (-) Length   405 a.a.
NCBI ID   WP_076029717.1    Uniprot ID   -
Organism   Pseudomonas frederiksbergensis strain AS1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3375903..3387120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PFAS1_RS15665 (PFAS1_15645) - 3376540..3377994 (-) 1455 WP_076029715.1 TrkH family potassium uptake protein -
  PFAS1_RS15670 (PFAS1_15650) - 3378240..3378929 (+) 690 WP_074870976.1 energy-coupling factor ABC transporter permease -
  PFAS1_RS15675 (PFAS1_15655) - 3378993..3379208 (+) 216 WP_008153324.1 hypothetical protein -
  PFAS1_RS15680 (PFAS1_15660) yacG 3379212..3379412 (-) 201 WP_008153323.1 DNA gyrase inhibitor YacG -
  PFAS1_RS15685 (PFAS1_15665) coaE 3379409..3380032 (-) 624 WP_074870974.1 dephospho-CoA kinase -
  PFAS1_RS15690 (PFAS1_15670) pilD 3380029..3380901 (-) 873 WP_076029716.1 prepilin peptidase Machinery gene
  PFAS1_RS15695 (PFAS1_15675) pilC 3380903..3382120 (-) 1218 WP_076029717.1 type II secretion system F family protein Machinery gene
  PFAS1_RS15700 (PFAS1_15680) pilB 3382123..3383823 (-) 1701 WP_076029718.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PFAS1_RS15705 (PFAS1_15685) - 3384049..3384477 (+) 429 WP_076029719.1 pilin -
  PFAS1_RS15710 (PFAS1_15690) - 3385211..3385564 (+) 354 WP_076029720.1 BON domain-containing protein -
  PFAS1_RS15715 (PFAS1_15695) - 3385848..3386147 (-) 300 WP_076029721.1 DUF2845 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44293.13 Da        Isoelectric Point: 9.7456

>NTDB_id=208039 PFAS1_RS15695 WP_076029717.1 3380903..3382120(-) (pilC) [Pseudomonas frederiksbergensis strain AS1]
MAVKAAKISVYAWEGTDRKGTRMTGELSGQNPALIKAQLRKQGINPGKVRKKSTSIFNLGKRIKAQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRALIDEVKQEVAAGNSFAASLRKKPQYFDELYCNLVDAGEQAGALDTLLERVATYKEKS
ESLKAKIKKAMTYPLAVVFVAIIVTGILLVKVVPQFESVFKGFGAELPAFTVMVIGLSEFMQDWWWAMLGVLIAAIFGVR
HAFKTSQGFRDRMDTWLLKMPLIGTLMYKSAVARYARTLSTTFAAGVPLVEALDSVAGATGNIVFKRAVLRIKQDVSTGM
QLNFSMRASGIFPSMAIQMTAIGEESGALDDMLDKVASFYEDEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=208039 PFAS1_RS15695 WP_076029717.1 3380903..3382120(-) (pilC) [Pseudomonas frederiksbergensis strain AS1]
ATGGCGGTCAAAGCAGCAAAAATCAGCGTCTACGCCTGGGAAGGCACCGACCGCAAAGGCACTAGAATGACGGGCGAGTT
GAGCGGCCAGAACCCGGCGTTGATCAAGGCGCAGTTGCGCAAGCAGGGCATCAACCCCGGCAAGGTGCGCAAGAAGTCCA
CCTCGATTTTCAACCTGGGCAAGCGCATCAAGGCCCAGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGAAG
GCCGGCGTACCGCTGCTGCAGTCCTTCGACATCATCGGCGAAGGCTTCGAAAACCCGGCCATGCGCGCGCTGATCGACGA
GGTGAAACAGGAAGTCGCGGCGGGTAACAGCTTCGCCGCCTCCTTGCGCAAAAAACCTCAGTATTTCGACGAGCTCTACT
GCAACCTGGTCGATGCCGGCGAGCAGGCCGGTGCCCTCGATACCCTGCTGGAAAGAGTGGCGACCTATAAGGAAAAAAGC
GAAAGCCTCAAGGCCAAGATCAAGAAAGCCATGACCTACCCGCTGGCGGTGGTGTTCGTCGCGATCATCGTGACCGGGAT
CCTGCTGGTCAAAGTGGTGCCGCAGTTCGAATCGGTATTCAAAGGGTTTGGCGCCGAATTGCCAGCCTTCACGGTGATGG
TCATCGGCCTCTCGGAATTCATGCAGGACTGGTGGTGGGCGATGCTCGGCGTGCTGATCGCGGCCATCTTCGGCGTGCGC
CACGCGTTTAAAACGTCGCAAGGCTTTCGCGACCGAATGGACACCTGGCTGCTGAAAATGCCGCTGATTGGCACGCTGAT
GTACAAGTCCGCCGTGGCCCGTTACGCCCGCACACTGTCGACGACTTTTGCGGCAGGCGTGCCACTGGTGGAAGCCCTGG
ATTCGGTGGCCGGCGCTACCGGCAACATTGTGTTCAAGCGCGCGGTGCTGCGGATCAAGCAGGACGTGTCGACCGGTATG
CAGCTGAATTTTTCCATGCGCGCCTCCGGCATTTTTCCGAGTATGGCGATCCAGATGACCGCCATCGGCGAAGAGTCCGG
CGCATTGGATGACATGCTCGACAAGGTCGCGAGCTTCTATGAGGACGAAGTGGACAACATGGTCGATAACCTCACCAGCC
TGATGGAGCCGTTCATCATGGTGGTGCTGGGTGTTATCGTCGGGGGCCTGGTGGTTGCGATGTACCTGCCCATCTTCCAA
CTCGGCACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

72.593

100

0.726
  pilC Acinetobacter baylyi ADP1

59.559

100

0.6
  pilC Acinetobacter baumannii D1279779

58.333

100

0.588
  pilC Legionella pneumophila strain ERS1305867

53.535

97.778

0.523
  pilG Neisseria gonorrhoeae MS11

43.035

99.259

0.427
  pilG Neisseria meningitidis 44/76-A

42.537

99.259

0.422
  pilC Vibrio cholerae strain A1552

42.394

99.012

0.42
  pilC Vibrio campbellii strain DS40M4

40.547

99.259

0.402
  pilC Thermus thermophilus HB27

38.404

99.012

0.38

Multiple sequence alignment