Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PFAS1_RS15700 Genome accession   NZ_CP018319
Coordinates   3382123..3383823 (-) Length   566 a.a.
NCBI ID   WP_076029718.1    Uniprot ID   -
Organism   Pseudomonas frederiksbergensis strain AS1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3377123..3388823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PFAS1_RS15670 (PFAS1_15650) - 3378240..3378929 (+) 690 WP_074870976.1 energy-coupling factor ABC transporter permease -
  PFAS1_RS15675 (PFAS1_15655) - 3378993..3379208 (+) 216 WP_008153324.1 hypothetical protein -
  PFAS1_RS15680 (PFAS1_15660) yacG 3379212..3379412 (-) 201 WP_008153323.1 DNA gyrase inhibitor YacG -
  PFAS1_RS15685 (PFAS1_15665) coaE 3379409..3380032 (-) 624 WP_074870974.1 dephospho-CoA kinase -
  PFAS1_RS15690 (PFAS1_15670) pilD 3380029..3380901 (-) 873 WP_076029716.1 prepilin peptidase Machinery gene
  PFAS1_RS15695 (PFAS1_15675) pilC 3380903..3382120 (-) 1218 WP_076029717.1 type II secretion system F family protein Machinery gene
  PFAS1_RS15700 (PFAS1_15680) pilB 3382123..3383823 (-) 1701 WP_076029718.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PFAS1_RS15705 (PFAS1_15685) - 3384049..3384477 (+) 429 WP_076029719.1 pilin -
  PFAS1_RS15710 (PFAS1_15690) - 3385211..3385564 (+) 354 WP_076029720.1 BON domain-containing protein -
  PFAS1_RS15715 (PFAS1_15695) - 3385848..3386147 (-) 300 WP_076029721.1 DUF2845 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62770.89 Da        Isoelectric Point: 5.5652

>NTDB_id=208040 PFAS1_RS15700 WP_076029718.1 3382123..3383823(-) (pilB) [Pseudomonas frederiksbergensis strain AS1]
MNDIALSGLAKQLVLAELLTDQSAQQAYQQAQRSRISLISYLVQNKLVKSRQVAEIASEHFGMALLDLNCLDKESQPKGL
ISEKLIRQHHALPLWRRGNKLFVGVSDPTNQQAINDIQFSTGLNTEAILVEDDKLSDAIEKFFDNQGSGLEDMADVDLDG
VDIESIDDNRQDAIAGQDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKTYRVRMRTDGMLREVAKPPIHLASRIAARL
KVMASLDISERRRPQDGRIKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEQDQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGMDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSISLIIAQRLARKLCSHCKKPIEIPREALLKEGFPE
ERIGSFTIYEPVGCDLCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDIQMRKDGFNDLRTSGLLKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=208040 PFAS1_RS15700 WP_076029718.1 3382123..3383823(-) (pilB) [Pseudomonas frederiksbergensis strain AS1]
ATGAATGACATTGCCCTTAGCGGTCTGGCCAAGCAATTGGTCCTGGCCGAGCTGCTCACTGACCAAAGCGCGCAACAGGC
GTATCAGCAAGCCCAGCGCAGCCGAATCTCCCTCATCAGCTATCTGGTGCAGAACAAACTGGTGAAGAGTCGCCAGGTCG
CCGAGATCGCGTCGGAGCACTTCGGCATGGCCTTGCTCGACCTCAACTGCCTGGACAAGGAGAGCCAGCCCAAGGGACTG
ATCAGCGAGAAACTGATTCGCCAGCACCACGCCCTGCCCCTCTGGCGGCGCGGCAACAAGTTGTTCGTGGGGGTTTCCGA
CCCGACCAATCAGCAAGCCATCAATGACATCCAGTTCAGCACCGGGCTGAATACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGAAAAATTCTTCGATAACCAAGGCTCCGGCCTGGAAGACATGGCTGACGTTGACCTCGATGGT
GTGGACATCGAGTCGATCGATGACAACAGACAAGACGCCATTGCCGGCCAGGATGCCGATGACGCGCCCGTGGTGCGCTT
CGTCCACAAGATGTTGCTGGACGCGATCAAAAGCGGCTCTTCCGACCTGCATTTCGAACCCTACGAAAAAACCTACCGAG
TGCGGATGCGCACCGACGGAATGTTGCGTGAAGTGGCCAAGCCACCGATCCACCTGGCCAGTCGCATCGCCGCGCGGCTG
AAAGTCATGGCCAGCCTCGACATCTCGGAGCGACGCCGGCCCCAGGACGGGCGGATCAAGATGCGCCTGTCCAAGACCAA
GTCCATCGATTTTCGGGTCAACACCCTGCCGACCCTGTGGGGCGAGAAAGTGGTGATCCGGATCCTCGACCCGTCCAGCG
CACAAATGGGCATCGATGCCCTCGGTTACGAGCAGGACCAGAAAGACCTGTACATGGCCGCGCTGAAGCAACCCCAGGGC
ATGATTCTGGTGACAGGCCCCACCGGGTCGGGCAAGACCGTATCGCTCTACACCGGGCTGAACATTCTCAACACCGTGGA
CATCAACATCTCCACCGCCGAAGACCCGGTAGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAAGCAGG
GAATGGACTTCGCCCAGGCGCTGCGCTCATTTCTGCGACAGGACCCGGACGTGATCATGGTCGGCGAAATCCGCGACCTC
GAAACCGCCGAAATTGCCATCAAAGCCGCCCAGACCGGTCACCTCGTGCTGTCGACCCTGCACACCAACAGCGCCGCCGA
AACCCTGACGCGCCTGCACAACATGGGCATCCCGGGTTTCAACATTGCCACCTCGATCAGCCTGATCATTGCCCAGCGCC
TGGCGCGCAAGCTGTGCAGCCATTGCAAGAAACCCATCGAGATTCCCCGGGAAGCGCTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCTCATTCACGATCTATGAACCGGTCGGTTGCGATCTGTGCAACGGCGGTTACAAAGGGCGAGTGGGGAT
TTATGAAGTGGTGAAAAACACACCGGAGCTGCAACGGCTGATCATGGCCGAAGGCAATTCACTGGAAATCGACATCCAGA
TGCGTAAAGACGGCTTCAACGACCTGCGCACGTCGGGCCTGCTCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.437

99.47

0.581
  pilB Acinetobacter baumannii D1279779

57.624

99.647

0.574
  pilB Legionella pneumophila strain ERS1305867

55.575

99.823

0.555
  pilB Vibrio cholerae strain A1552

51.658

100

0.523
  pilB Vibrio parahaemolyticus RIMD 2210633

51.259

98.233

0.504
  pilB Vibrio campbellii strain DS40M4

50

100

0.504
  pilF Neisseria gonorrhoeae MS11

49.556

99.47

0.493
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.983

93.816

0.366

Multiple sequence alignment