Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BSZ05_RS04170 Genome accession   NZ_CP018308
Coordinates   927112..928803 (+) Length   563 a.a.
NCBI ID   WP_173670880.1    Uniprot ID   A0AAN1FJG3
Organism   Vibrio mediterranei strain QT6D1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 922112..933803
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSZ05_RS04150 (BSZ05_04140) pdhR 923629..924396 (-) 768 WP_006074432.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  BSZ05_RS04155 (BSZ05_04145) ampD 924781..925365 (-) 585 WP_088876257.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BSZ05_RS04160 (BSZ05_04150) nadC 925501..926391 (+) 891 WP_088876258.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BSZ05_RS04165 (BSZ05_04155) - 926633..927112 (+) 480 WP_088876259.1 pilin -
  BSZ05_RS04170 (BSZ05_04160) pilB 927112..928803 (+) 1692 WP_173670880.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BSZ05_RS04175 (BSZ05_04165) pilC 928814..930040 (+) 1227 WP_088876261.1 type II secretion system F family protein Machinery gene
  BSZ05_RS04180 (BSZ05_04170) pilD 930083..930952 (+) 870 WP_088876262.1 prepilin peptidase Machinery gene
  BSZ05_RS04185 (BSZ05_04175) coaE 930955..931563 (+) 609 WP_088876263.1 dephospho-CoA kinase -
  BSZ05_RS04190 (BSZ05_04180) zapD 931595..932335 (+) 741 WP_031494891.1 cell division protein ZapD -
  BSZ05_RS04195 (BSZ05_04185) yacG 932357..932551 (+) 195 WP_006074419.1 DNA gyrase inhibitor YacG -
  BSZ05_RS04200 (BSZ05_04190) rplS 932750..933103 (-) 354 WP_006074418.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62630.98 Da        Isoelectric Point: 5.8095

>NTDB_id=207937 BSZ05_RS04170 WP_173670880.1 927112..928803(+) (pilB) [Vibrio mediterranei strain QT6D1]
MPSTLPSILRQANAIDETQEAALYDLSMLSGCSVPEGATELNLCQAQELVAVLAEVFGLPIVSIKDNDYARLCQQLGLRD
VITQYQALPILLRDNVLTVAVSDPTPSQLESDFKFASGLQIELSLANHQDLNAAIRKIYGEGQEQWSQQTKELTSHELAN
LVQVNESELEHYDELMLDDSPVSRFIHQILLEAVSKKASDIHFEPYEHQYRIRLRCDGILIEAHQPSIQLSRRLSARLKI
LAKLDIAERRLPQDGRIKLKLSPHSATDIRVSTLPTMWGEKVVLRILDLNSARLDIEQLGYNDTQQTLYLQALRKPQGMV
LMTGPTGSGKTVSLYAGLKLLNTPERNISTAEDPIEIHLDGINQVQVNPKIGFGFSAALRTFLRQDPDVVMVGEIRDLDT
AEIAVKAAQTGHLVLSTLHTNSALETITRLQNMGIESFNLVSSLSLIIAQRLARRLCSLCKRPDHLPKDLLRSLDISSCQ
RIYKANDVGCSKCNRGYSGRIGIYEVLVFSNELSAAVLQKASIQDLEVIAVSQGMMTLKLAGLEKLTLGLTSYQELQRVL
YFE

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=207937 BSZ05_RS04170 WP_173670880.1 927112..928803(+) (pilB) [Vibrio mediterranei strain QT6D1]
ATCCCGTCCACTCTTCCATCTATTTTAAGACAGGCTAACGCCATCGATGAAACGCAAGAAGCCGCGTTATATGACCTGTC
CATGCTATCTGGTTGCTCCGTTCCAGAAGGAGCAACTGAGCTCAACCTTTGTCAGGCGCAAGAGCTTGTTGCTGTTCTAG
CCGAAGTTTTTGGACTCCCAATAGTGTCGATAAAGGACAACGACTATGCGAGATTGTGCCAACAACTCGGTTTACGGGAC
GTCATAACTCAATACCAAGCCTTACCTATTTTACTGCGCGACAACGTTCTAACCGTTGCGGTCAGCGATCCCACACCTTC
GCAGTTAGAAAGTGATTTTAAGTTTGCCTCAGGATTACAAATCGAACTGAGCTTGGCTAACCATCAAGACCTAAACGCCG
CAATACGCAAAATTTATGGAGAGGGTCAAGAGCAATGGAGCCAACAGACTAAGGAACTCACCTCCCACGAACTGGCAAAC
TTAGTACAAGTGAATGAGAGTGAACTTGAGCATTACGATGAGCTAATGCTGGATGACTCGCCTGTCAGCCGTTTCATTCA
CCAAATCCTATTAGAAGCCGTCAGCAAGAAAGCGTCCGATATTCATTTTGAGCCATACGAACACCAGTATCGAATTCGAC
TTCGCTGTGATGGTATTCTTATTGAGGCACATCAGCCATCGATCCAATTAAGCCGCAGACTATCTGCTCGCCTGAAGATT
CTCGCTAAGCTAGATATTGCTGAACGCCGTCTTCCTCAAGATGGTCGAATCAAACTCAAACTATCCCCCCACTCCGCCAC
GGATATCCGGGTATCAACCCTGCCAACCATGTGGGGAGAAAAGGTAGTACTGAGAATTCTCGACCTAAATTCTGCCCGTT
TGGATATCGAGCAATTAGGGTATAACGACACTCAACAAACCCTTTACTTGCAAGCGTTACGCAAGCCTCAAGGAATGGTG
TTAATGACAGGGCCTACTGGAAGTGGCAAAACCGTCTCTCTCTATGCGGGACTCAAACTTCTTAATACCCCAGAGCGAAA
TATCTCAACTGCCGAAGACCCGATTGAAATACATCTAGATGGGATAAACCAAGTTCAAGTTAACCCTAAGATAGGCTTTG
GTTTTTCTGCAGCCTTGCGCACTTTTTTACGCCAAGATCCGGATGTGGTTATGGTGGGAGAAATACGAGATCTCGACACA
GCAGAGATTGCGGTTAAAGCGGCTCAAACAGGTCATCTAGTTCTGTCAACTTTGCACACTAATTCCGCGCTTGAGACCAT
TACACGGCTACAAAATATGGGAATAGAGAGCTTCAACCTCGTGTCTTCACTTTCGCTAATTATCGCGCAGCGGCTCGCTC
GCCGTTTGTGCTCTTTATGTAAGCGCCCAGATCACCTCCCTAAAGATCTGCTCCGCAGTCTCGATATATCCTCCTGCCAG
AGGATTTACAAAGCCAATGATGTTGGTTGTAGCAAATGTAATAGAGGCTATTCGGGTCGTATCGGTATTTACGAAGTGCT
GGTTTTTTCCAACGAGTTAAGCGCCGCCGTTCTTCAGAAAGCCAGTATTCAAGATCTTGAAGTCATCGCTGTGTCACAAG
GAATGATGACACTTAAACTAGCGGGGCTTGAAAAACTCACTCTTGGACTGACAAGCTACCAAGAACTACAGCGTGTCCTT
TATTTCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

63.894

100

0.641
  pilB Vibrio campbellii strain DS40M4

61.566

99.822

0.615
  pilB Vibrio parahaemolyticus RIMD 2210633

60.924

100

0.609
  pilB Legionella pneumophila strain ERS1305867

45.57

98.224

0.448
  pilB Acinetobacter baumannii D1279779

46.923

92.362

0.433
  pilB Acinetobacter baylyi ADP1

47.732

90.053

0.43
  pilF Neisseria gonorrhoeae MS11

44.208

92.007

0.407
  pilB Haemophilus influenzae Rd KW20

40

94.139

0.377
  pilB Haemophilus influenzae 86-028NP

39.163

93.428

0.366
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.535

91.652

0.362

Multiple sequence alignment