Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   BM528_RS14090 Genome accession   NZ_CP018031
Coordinates   3168616..3169824 (+) Length   402 a.a.
NCBI ID   WP_071980366.1    Uniprot ID   A0AAC9NVV7
Organism   Alteromonas sp. RW2A1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3163616..3174824
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BM528_RS14060 (BM528_13845) ampD 3163711..3164250 (-) 540 WP_071980360.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BM528_RS14065 (BM528_13850) - 3164345..3165571 (-) 1227 WP_071980361.1 RNA-guided endonuclease TnpB family protein -
  BM528_RS14070 (BM528_13855) tnpA 3165610..3165993 (+) 384 WP_071980362.1 IS200/IS605 family transposase -
  BM528_RS14075 (BM528_13860) - 3166278..3166796 (+) 519 WP_071980363.1 TIGR02281 family clan AA aspartic protease -
  BM528_RS14080 (BM528_13865) nadC 3166830..3167684 (+) 855 WP_198033618.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BM528_RS14085 (BM528_13870) - 3168117..3168545 (+) 429 WP_071980365.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  BM528_RS14090 (BM528_13875) pilC 3168616..3169824 (+) 1209 WP_071980366.1 type II secretion system F family protein Machinery gene
  BM528_RS14095 (BM528_13880) pilD 3169987..3170895 (+) 909 WP_071980367.1 A24 family peptidase Machinery gene
  BM528_RS14100 (BM528_13885) coaE 3170896..3171501 (+) 606 WP_071980368.1 dephospho-CoA kinase -
  BM528_RS14105 (BM528_13890) zapD 3171900..3172652 (+) 753 WP_071980369.1 cell division protein ZapD -
  BM528_RS14110 (BM528_13895) yacG 3172902..3173129 (+) 228 WP_071980370.1 DNA gyrase inhibitor YacG -
  BM528_RS14115 (BM528_13900) - 3173151..3174047 (+) 897 WP_071980371.1 aspartoacylase -
  BM528_RS14120 (BM528_13905) mutT 3174237..3174629 (-) 393 WP_071980372.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43426.98 Da        Isoelectric Point: 9.2967

>NTDB_id=205165 BM528_RS14090 WP_071980366.1 3168616..3169824(+) (pilC) [Alteromonas sp. RW2A1]
MKTAAVFEWQGKDRHGAGRKGEISAASLSEAKNLLRRQGISANKVKKQSKPLFGSGAAKITAADISVTSRQIATMLSAGV
TLIQSLEMIAQGHAKVSMRKLLGEITEEVKAGNPLSSALRKHPNYFDDLYCDLVYTGEQSGALELIYDRIATYKEKAEAL
KSKIKKAMFYPIAVVVVAFIVTTILLVFVVPQFEEIFSSFGAELPAFTQFVLGISRFVQDYGIFIAIAVGIAAFLFGRAY
KKSQKLRDSCDRFVLKIPVIGEILRKASIARFTRTLATTFAAGVPLIGALESAAGASGNAVYRDAILFMRKEVAGGIPMH
VAMRATNVFPDMVTQMIAIGEESGAVDEMLSKIATIYEAEVDDMVDGLTSLLEPMIMAVLGVVIGGLIVAMYLPIFDMGN
VV

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=205165 BM528_RS14090 WP_071980366.1 3168616..3169824(+) (pilC) [Alteromonas sp. RW2A1]
GTGAAAACAGCAGCTGTTTTTGAATGGCAAGGTAAAGACCGGCACGGTGCGGGTAGGAAAGGTGAAATTTCAGCGGCATC
ACTTTCGGAAGCTAAGAACCTTTTACGTAGACAGGGAATATCAGCGAACAAAGTAAAAAAACAAAGTAAACCTTTGTTTG
GCTCTGGCGCTGCAAAAATAACTGCGGCAGATATTTCTGTCACCTCTAGGCAAATAGCCACTATGCTTAGCGCTGGTGTT
ACCCTTATACAATCTCTTGAGATGATTGCCCAAGGCCATGCAAAAGTTAGCATGCGCAAGTTGCTTGGCGAGATTACCGA
GGAAGTAAAAGCCGGTAACCCTTTATCTTCAGCATTGAGAAAACACCCCAACTATTTCGATGATTTGTATTGCGACCTTG
TGTATACCGGTGAGCAGTCTGGAGCCCTCGAGCTTATTTACGACCGAATAGCCACATACAAGGAAAAAGCCGAAGCTTTA
AAATCTAAAATCAAAAAGGCGATGTTTTATCCTATAGCTGTGGTTGTAGTTGCGTTTATTGTAACTACTATTCTTCTTGT
TTTTGTTGTTCCACAGTTTGAAGAGATATTCAGCAGTTTTGGTGCTGAACTACCGGCCTTCACGCAGTTTGTTCTAGGTA
TTTCTCGCTTCGTGCAAGATTACGGTATCTTTATCGCAATTGCTGTAGGTATTGCAGCCTTTTTGTTTGGCCGTGCTTAT
AAGAAAAGTCAAAAGCTGCGGGATTCTTGTGATAGATTCGTTTTAAAAATTCCTGTGATTGGGGAAATTCTCAGAAAAGC
CAGTATTGCTCGCTTTACCCGTACACTTGCAACAACATTCGCCGCTGGTGTACCTCTGATAGGCGCACTTGAATCTGCTG
CCGGAGCCTCGGGTAATGCAGTATATCGTGATGCTATCTTATTTATGCGTAAAGAAGTAGCAGGCGGTATACCCATGCAT
GTGGCTATGCGTGCGACTAACGTATTCCCCGATATGGTGACGCAGATGATTGCTATTGGCGAAGAGTCTGGTGCTGTAGA
TGAAATGCTAAGCAAGATTGCCACTATCTATGAAGCGGAAGTGGATGACATGGTAGACGGCTTAACTAGCTTACTAGAGC
CTATGATTATGGCTGTACTAGGTGTAGTTATCGGTGGTCTTATTGTTGCTATGTACCTGCCTATATTTGATATGGGTAAC
GTGGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(69-191)

Type II secretion system (T2SS), protein F (T2SSF)

(272-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

58.04

99.005

0.575
  pilC Acinetobacter baumannii D1279779

52.897

98.756

0.522
  pilC Acinetobacter baylyi ADP1

52.513

99.005

0.52
  pilC Legionella pneumophila strain ERS1305867

51.768

98.507

0.51
  pilC Vibrio cholerae strain A1552

46.465

98.507

0.458
  pilG Neisseria meningitidis 44/76-A

45.57

98.259

0.448
  pilG Neisseria gonorrhoeae MS11

45.316

98.259

0.445
  pilC Vibrio campbellii strain DS40M4

43.829

98.756

0.433
  pilC Thermus thermophilus HB27

36.908

99.751

0.368

Multiple sequence alignment