Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   BM526_RS13690 Genome accession   NZ_CP018029
Coordinates   3161943..3163151 (+) Length   402 a.a.
NCBI ID   WP_071969409.1    Uniprot ID   -
Organism   Alteromonas mediterranea strain RG65     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3156943..3168151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BM526_RS13665 (BM526_13610) ampE 3157912..3158781 (-) 870 WP_015067808.1 beta-lactamase regulator AmpE -
  BM526_RS13670 (BM526_13615) ampD 3158812..3159348 (-) 537 WP_071969403.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BM526_RS13675 (BM526_13620) - 3159524..3160042 (+) 519 WP_012519426.1 TIGR02281 family clan AA aspartic protease -
  BM526_RS13680 (BM526_13625) nadC 3160062..3160916 (+) 855 WP_015067810.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BM526_RS13685 (BM526_13630) - 3161452..3161871 (+) 420 WP_071969405.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  BM526_RS13690 (BM526_13635) pilC 3161943..3163151 (+) 1209 WP_071969409.1 type II secretion system F family protein Machinery gene
  BM526_RS13695 (BM526_13640) pilD 3163281..3164174 (+) 894 WP_071969411.1 A24 family peptidase Machinery gene
  BM526_RS13700 (BM526_13645) coaE 3164196..3164810 (+) 615 WP_071969413.1 dephospho-CoA kinase -
  BM526_RS13705 (BM526_13650) zapD 3165024..3165776 (+) 753 WP_015067815.1 cell division protein ZapD -
  BM526_RS13710 (BM526_13655) yacG 3165867..3166094 (+) 228 WP_012519434.1 DNA gyrase inhibitor YacG -
  BM526_RS13715 (BM526_13660) - 3166287..3167159 (+) 873 WP_071969415.1 aspartoacylase -
  BM526_RS13720 (BM526_13665) mutT 3167250..3167633 (-) 384 WP_020746438.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43724.44 Da        Isoelectric Point: 9.5919

>NTDB_id=205147 BM526_RS13690 WP_071969409.1 3161943..3163151(+) (pilC) [Alteromonas mediterranea strain RG65]
MPKVATVFTWQGKDRHGQARKGEISATSLSEAKNLLRRQGISANKVKKLAKPLFGGAKKITPADISVVSRQIATMLAAGV
TLIQSLEMIAQGHANPSMRKLLGEITDEVKSGNPLSSALRKHPLYFDDLYCDLVYTGEQSGALETIYDRIATYKEKAEAL
KSKIKKAMFYPIAVLVVAFIVTTILLIFVVPQFEEIFSSFGAELPAFTQFVLAISRFVQDYGIFIAMGFAAAGFMFVRAH
RRSQKLRDTVDRNILKIPVIGEILKKASIARFTRTLATTFAAGVPLIGALESAAGASGNAVYRDAILYIRKEVAGGMPMH
VAMRTTQVFPDMVTQMIAIGEESGAVDEMLSKIATIYEAEVDDMVDGLTSLLEPMIMAVLGVVIGGLIVAMYLPIFEMGN
VV

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=205147 BM526_RS13690 WP_071969409.1 3161943..3163151(+) (pilC) [Alteromonas mediterranea strain RG65]
ATGCCAAAGGTAGCTACGGTATTCACTTGGCAAGGCAAAGATCGCCACGGTCAAGCTCGAAAAGGCGAAATTTCAGCCAC
ATCACTTTCCGAAGCCAAAAACCTCTTACGCCGCCAAGGTATCTCTGCAAACAAAGTCAAAAAGCTTGCCAAACCACTTT
TCGGTGGCGCAAAGAAAATTACGCCAGCGGATATTTCTGTCGTATCTCGTCAAATCGCCACCATGCTTGCCGCAGGTGTT
ACGCTTATCCAATCTTTAGAAATGATAGCGCAGGGCCATGCAAACCCTTCCATGCGTAAACTGCTTGGTGAAATCACCGA
TGAAGTCAAATCCGGTAACCCACTGTCATCGGCACTTAGAAAGCACCCCCTATATTTTGACGACCTGTATTGTGATTTGG
TTTACACCGGCGAACAGTCAGGTGCCCTTGAAACTATTTACGACCGTATTGCCACCTATAAAGAAAAAGCCGAAGCACTA
AAATCAAAGATCAAAAAAGCGATGTTTTACCCTATCGCGGTTTTGGTTGTTGCTTTTATTGTTACGACTATCCTTCTTAT
TTTCGTTGTGCCTCAGTTTGAAGAAATCTTTAGCAGCTTTGGCGCTGAACTCCCTGCGTTTACCCAGTTCGTACTGGCTA
TATCGCGGTTTGTACAGGATTACGGTATTTTTATCGCCATGGGTTTTGCTGCGGCAGGATTTATGTTTGTGCGTGCACAC
AGACGTAGCCAAAAGCTGCGCGATACGGTAGACCGTAATATTTTAAAAATTCCTGTTATCGGCGAAATACTCAAAAAAGC
CAGTATCGCACGCTTTACCCGTACTTTGGCCACCACCTTTGCGGCAGGTGTACCGCTAATAGGCGCGTTAGAGTCGGCAG
CGGGTGCATCGGGTAACGCTGTTTATCGAGACGCGATACTATATATACGCAAAGAAGTGGCCGGTGGTATGCCTATGCAT
GTAGCCATGCGTACTACACAAGTATTTCCTGACATGGTGACCCAAATGATTGCCATCGGTGAAGAGTCGGGCGCGGTAGA
TGAAATGCTAAGTAAAATTGCCACCATTTACGAAGCAGAAGTTGATGACATGGTAGACGGCTTAACCAGCTTGCTTGAGC
CTATGATCATGGCCGTACTTGGCGTGGTTATTGGCGGTTTGATTGTGGCCATGTACCTGCCTATATTCGAAATGGGTAAC
GTGGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(272-394)

Type II secretion system (T2SS), protein F (T2SSF)

(69-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

59.95

98.756

0.592
  pilC Legionella pneumophila strain ERS1305867

53.518

99.005

0.53
  pilC Acinetobacter baylyi ADP1

52.736

100

0.527
  pilC Acinetobacter baumannii D1279779

53.149

98.756

0.525
  pilG Neisseria gonorrhoeae MS11

45.5

99.502

0.453
  pilG Neisseria meningitidis 44/76-A

45.5

99.502

0.453
  pilC Vibrio cholerae strain A1552

45.455

98.507

0.448
  pilC Vibrio campbellii strain DS40M4

44.11

99.254

0.438
  pilC Thermus thermophilus HB27

37.157

99.751

0.371

Multiple sequence alignment