Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BJD94_RS15275 Genome accession   NZ_CP017635
Coordinates   3019024..3020712 (-) Length   562 a.a.
NCBI ID   WP_038964708.1    Uniprot ID   -
Organism   Vibrio vulnificus Env1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3014024..3025712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJD94_RS15245 (BJD94_13950) rplS 3014562..3014915 (+) 354 WP_011079544.1 50S ribosomal protein L19 -
  BJD94_RS15250 (BJD94_13955) yacG 3015147..3015341 (-) 195 WP_038941426.1 DNA gyrase inhibitor YacG -
  BJD94_RS15255 (BJD94_13960) zapD 3015404..3016144 (-) 741 WP_017421189.1 cell division protein ZapD -
  BJD94_RS15260 (BJD94_13965) coaE 3016177..3016785 (-) 609 WP_038964346.1 dephospho-CoA kinase -
  BJD94_RS15265 (BJD94_13970) pilD 3016787..3017656 (-) 870 WP_080526796.1 A24 family peptidase Machinery gene
  BJD94_RS15270 (BJD94_13975) pilC 3017750..3018976 (-) 1227 WP_038964709.1 type II secretion system F family protein Machinery gene
  BJD94_RS15275 (BJD94_13980) pilB 3019024..3020712 (-) 1689 WP_038964708.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BJD94_RS15280 (BJD94_13985) pilA 3020717..3021142 (-) 426 WP_038964707.1 pilin Machinery gene
  BJD94_RS15285 (BJD94_13990) nadC 3021365..3022252 (-) 888 WP_017421195.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BJD94_RS15290 (BJD94_13995) ampD 3022345..3022917 (+) 573 WP_103154530.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BJD94_RS15295 (BJD94_14000) pdhR 3023306..3024073 (+) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62458.48 Da        Isoelectric Point: 5.6848

>NTDB_id=200838 BJD94_RS15275 WP_038964708.1 3019024..3020712(-) (pilB) [Vibrio vulnificus Env1]
MQSNLATILRQANQLSLTQEQACRETIQASGVTAPEALLQLGFFQPDELTEKLSAIFGLPRTDLNQYDYQELCNQLGLRE
LITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQDLQAAIRRLYGKSIKQSQGSGLKEIHQDELA
NLVEVSDDEIESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGM
ILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
LIYQADKQGCHHCTHGYSGRVGIYEVMPFNEQLAEAIVKDASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVL
YL

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=200838 BJD94_RS15275 WP_038964708.1 3019024..3020712(-) (pilB) [Vibrio vulnificus Env1]
GTGCAATCCAACCTTGCCACGATTCTGCGTCAGGCCAACCAACTTAGCCTAACGCAAGAACAAGCGTGTCGAGAGACAAT
ACAAGCTTCTGGTGTGACAGCACCGGAAGCTTTGTTGCAACTGGGATTTTTCCAGCCTGATGAACTAACCGAAAAGCTAA
GTGCGATTTTTGGCTTACCACGCACTGACCTCAACCAATATGACTACCAAGAGCTGTGTAATCAACTTGGGTTGCGTGAG
CTGATCACTCGTCACCAAGCTCTGCCACTGCAAAAAAGCAACGCTGGTTTAATACTCGCGGTTGCAGACCCGACCAATAT
TCAAGCTGAAGATGATTTCCGTTTTGCCACAGGCGTGCAGGTGGAGATTGTTTTAGCCGATTGTCAAGATCTGCAAGCGG
CGATTCGTAGACTCTATGGAAAGAGCATCAAACAGAGCCAAGGCTCTGGTTTAAAAGAGATTCACCAAGATGAGCTGGCG
AATTTGGTCGAGGTTTCTGATGACGAAATCGAGTCCATTGAAGACTTAAGCCAAGATGAATCCCCCGTTAGCCGTTTTAT
CAACCAAATTCTGCTTGATGCGGTGCGCAAAGGCGCATCTGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTAC
GATTGCGTTGCGATGGTATTTTGGTGGAAGTGCAGCAGCCTCCAAGCCATTTAAGCCGCCGTTTATCCGCGAGGATCAAA
ATCCTCTCCAAGCTGGATATTGCGGAAAGGCGTCTGCCACAAGATGGACGCATTAAGCTACGCCTTAACCAAGAAACCGC
GATTGATATGCGCGTTTCCACTTTACCTACGCTGTTTGGTGAAAAAATTGTGCTGCGACTTCTCGACAGCTCGAGTAGCA
GCTTGGATATTGATAAGTTAGGCTACAACCCGCAACAAAAGCAGCTCTACCTCGATGCCCTGCGCAAACCGCAAGGGATG
ATTTTGATGACAGGTCCAACAGGAAGCGGCAAAACCGTTTCTCTGTACACGGGCTTGAGTTTGCTTAACCGACCTGAAGT
GAATATCTCCACCGCGGAAGACCCGGTCGAGATTAACCTCCCAGGTATCAACCAAGTGCAAGTGAAACCCAAAATTGGTT
TTGGTTTTGCCGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCGGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAA
ACCGCCGAGATCGCCGTCAAAGCAGCCCAAACCGGCCACCTTGTGCTATCCACCTTACATACCAACTCCGCTGCCGAAAC
CATTGTTCGTCTATCTAACATGGGGATCGCCAGCTTTAACCTGGCTTCATCCTTAAGTTTGATCATTGCTCAGCGTCTTG
CTCGTCGGTTATGCAGCCATTGCAAGCAACCGCATCCATACAACGCAACATTTGAGTTACTCGGCCTAAAAGCTGACGAC
TTGATTTATCAAGCCGATAAACAGGGTTGCCATCATTGTACTCATGGTTATTCCGGTCGTGTCGGTATATACGAAGTCAT
GCCTTTTAATGAGCAGCTAGCAGAGGCGATCGTTAAAGACGCCTCGGTACAAAGCTTGGAGATGCTTGCTCGGCAAAAAG
GCATGATGACACTCAAAGACTCTGGCCTCGAGAAACTCAAACAAGGCATCACTAGCTTAGAAGAGCTGCAACGCGTCCTA
TATTTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

76.157

100

0.762
  pilB Vibrio parahaemolyticus RIMD 2210633

75.623

100

0.756
  pilB Vibrio cholerae strain A1552

74.423

100

0.746
  pilB Legionella pneumophila strain ERS1305867

53.021

91.281

0.484
  pilB Acinetobacter baumannii D1279779

51.533

92.883

0.479
  pilB Acinetobacter baylyi ADP1

51.149

92.883

0.475
  pilF Neisseria gonorrhoeae MS11

45.31

100

0.456
  pilF Thermus thermophilus HB27

40.072

99.466

0.399
  pilB/pilB1 Synechocystis sp. PCC 6803

36.888

100

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.302

94.306

0.361

Multiple sequence alignment