Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   BJD94_RS14080 Genome accession   NZ_CP017635
Coordinates   2786773..2788557 (+) Length   594 a.a.
NCBI ID   WP_017419660.1    Uniprot ID   A0A087IG05
Organism   Vibrio vulnificus Env1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2781773..2793557
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJD94_RS14060 (BJD94_12780) pilM 2784087..2785094 (+) 1008 WP_103155248.1 type IV pilus assembly protein PilM Machinery gene
  BJD94_RS14065 (BJD94_12785) pilN 2785084..2785674 (+) 591 WP_038940289.1 PilN domain-containing protein Machinery gene
  BJD94_RS14070 (BJD94_12790) pilO 2785667..2786257 (+) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  BJD94_RS14075 (BJD94_12795) pilP 2786247..2786759 (+) 513 WP_038940290.1 pilus assembly protein PilP Machinery gene
  BJD94_RS14080 (BJD94_12800) pilQ 2786773..2788557 (+) 1785 WP_017419660.1 type IV pilus secretin PilQ Machinery gene
  BJD94_RS14085 (BJD94_12805) aroK 2788759..2789277 (+) 519 WP_011079357.1 shikimate kinase AroK -
  BJD94_RS14090 (BJD94_12810) aroB 2789310..2790401 (+) 1092 WP_038964151.1 3-dehydroquinate synthase -
  BJD94_RS14095 (BJD94_12815) - 2790452..2791948 (+) 1497 WP_017419661.1 AAA family ATPase -
  BJD94_RS14100 (BJD94_12820) - 2792028..2792855 (+) 828 WP_038964150.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 594 a.a.        Molecular weight: 64591.66 Da        Isoelectric Point: 4.5077

>NTDB_id=200826 BJD94_RS14080 WP_017419660.1 2786773..2788557(+) (pilQ) [Vibrio vulnificus Env1]
MICELMMKNKIQLFPGMLSQFVCLLSVCIFTTFANAQSEAENALKSIDFRTNAAKDAIIIVELATPATVVDLKPVAEGLS
IELINTSVKDEQLYLLDVKDFSTVVESIEVFREVSKTRLVANVSGEFRHDYRLSGRYLEVTISRIKSEQEAREQSVLEKE
GKLISINFQDIPVRNVLQLIADYNGFNLVVSDSVTGNVTLRLDGVPWQQVLDIILQVKGLDKRVEGNVVLIAPQDELDLR
EKQQLEKERLAEELGSLKSEIIKVNFAKASDIAEMINGKESVSMLSERGSVAIDERTNSLLLRELPENIEVIREIINSLD
IPVKQVQIEARIVTVNEGNLDELGVRWGFTSINGSTTVGGSIENNLATIGLYNAGSGSGSGDSGSGGSGDEGGSNSIDDF
LNVNLAATSANASSIAFQVAKLGSDLLLDLELSALQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVSF
KKAVLSLKVTPQITPDNRLVLDLSVTQDRPGEIVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNTTDKVPL
LGDLPLLGALFRRSYENVGKRELLIFVTPKVILQ

Nucleotide


Download         Length: 1785 bp        

>NTDB_id=200826 BJD94_RS14080 WP_017419660.1 2786773..2788557(+) (pilQ) [Vibrio vulnificus Env1]
GTGATTTGTGAGTTGATGATGAAAAACAAAATCCAATTGTTTCCGGGAATGCTTTCCCAATTCGTCTGCCTGTTGTCTGT
TTGCATCTTTACCACTTTTGCAAACGCACAATCTGAAGCAGAAAATGCGCTCAAGAGCATCGATTTCAGAACCAATGCAG
CCAAGGATGCGATTATCATCGTTGAATTGGCAACGCCTGCAACCGTGGTGGATTTGAAACCAGTGGCAGAAGGATTGAGC
ATCGAGTTGATCAATACCTCGGTTAAAGATGAGCAGCTCTATCTGCTCGATGTCAAAGATTTTTCGACCGTGGTTGAAAG
TATCGAAGTATTCAGAGAAGTGTCCAAAACCCGTCTTGTTGCGAATGTCAGCGGTGAATTTCGTCACGACTATCGTCTTT
CTGGACGCTATCTCGAGGTGACAATTTCTCGCATCAAATCGGAGCAAGAAGCCCGTGAACAAAGTGTGTTGGAAAAAGAA
GGCAAGCTGATTTCTATCAACTTCCAAGATATTCCAGTGCGTAACGTTTTGCAGCTTATCGCAGACTACAATGGCTTCAA
CCTCGTGGTGTCTGATTCTGTCACTGGTAATGTGACGCTGAGATTGGATGGGGTACCTTGGCAGCAAGTGCTCGACATCA
TTCTTCAAGTGAAAGGTCTAGACAAACGTGTTGAAGGCAATGTTGTGCTCATTGCTCCTCAAGATGAACTGGATTTGCGA
GAGAAGCAGCAGCTTGAGAAAGAACGTCTGGCGGAAGAGCTTGGCTCCCTCAAATCGGAAATCATCAAAGTCAACTTTGC
CAAAGCGTCAGACATTGCTGAGATGATCAATGGCAAAGAGTCGGTCAGCATGCTGTCAGAGCGTGGTAGTGTGGCGATTG
ATGAACGGACAAACTCTCTTTTATTGCGTGAGTTACCCGAAAACATCGAAGTGATTCGAGAAATTATCAATTCGTTGGAT
ATCCCAGTCAAACAGGTACAAATTGAAGCACGCATTGTCACGGTGAATGAAGGCAATCTTGATGAGCTTGGTGTCCGTTG
GGGATTCACCTCCATCAACGGCAGCACAACCGTCGGCGGTTCTATCGAAAACAACCTAGCAACCATTGGTCTCTACAATG
CAGGCAGTGGGAGTGGCAGTGGTGACTCAGGTAGCGGTGGCAGTGGTGATGAAGGCGGGTCGAACTCCATTGATGATTTC
CTCAATGTCAATCTTGCTGCGACTTCTGCAAACGCCTCAAGTATTGCTTTCCAAGTGGCTAAATTGGGGTCGGATTTGTT
GTTGGATCTCGAGCTATCAGCACTGCAACGCGAATCAAAAGCGGAAATTATTTCTAGCCCTCGTTTGATCACAACCAATA
AGAAGCCTGCTTATATCGAGCAAGGTACAGAGATTCCTTATCTAGAGTCTTCCTCCAGTGGAGCGACCACGGTTTCCTTT
AAGAAAGCGGTGCTTAGCCTGAAAGTAACCCCTCAGATCACGCCTGACAATCGTCTCGTGTTGGATTTAAGCGTTACACA
AGATAGACCTGGCGAAATTGTCAAAACAGGCACTGGGGAAGCGGTTGCGATTGATACTCAGCGCATCGGTACACAAGTCT
TGGTCAATAATGGTGAAACGGTAGTGTTAGGCGGTATTTTCCAACACAGCATAACCAATACCACGGACAAGGTTCCTCTG
TTAGGCGATTTACCCTTATTGGGTGCGCTTTTCCGCCGTAGCTATGAAAACGTGGGCAAGCGTGAATTGCTGATATTTGT
CACACCTAAGGTTATCTTGCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(260-325)

Bacterial type II and III secretion system protein (Secretin)

(434-592)

Secretin and TonB N terminus short domain (STN)

(186-234)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A087IG05

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

77.966

99.327

0.774
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

74.068

99.327

0.736
  pilQ Vibrio cholerae strain A1552

74.068

99.327

0.736
  pilQ Legionella pneumophila strain Lp02

41.429

94.276

0.391
  pilQ Legionella pneumophila strain ERS1305867

41.429

94.276

0.391
  pilQ Pseudomonas aeruginosa PAK

38.435

96.801

0.372
  comQ Acinetobacter baylyi ADP1

36.824

99.663

0.367

Multiple sequence alignment