Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   BJD94_RS14060 Genome accession   NZ_CP017635
Coordinates   2784087..2785094 (+) Length   335 a.a.
NCBI ID   WP_103155248.1    Uniprot ID   -
Organism   Vibrio vulnificus Env1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2779087..2790094
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJD94_RS14045 (BJD94_12765) - 2779920..2780192 (-) 273 WP_103155249.1 DUF3624 domain-containing protein -
  BJD94_RS14050 (BJD94_12770) oxyR 2780466..2781365 (+) 900 WP_045591363.1 DNA-binding transcriptional regulator OxyR -
  BJD94_RS14055 (BJD94_12775) - 2781434..2783947 (-) 2514 WP_017419655.1 penicillin-binding protein 1A -
  BJD94_RS14060 (BJD94_12780) pilM 2784087..2785094 (+) 1008 WP_103155248.1 type IV pilus assembly protein PilM Machinery gene
  BJD94_RS14065 (BJD94_12785) pilN 2785084..2785674 (+) 591 WP_038940289.1 PilN domain-containing protein Machinery gene
  BJD94_RS14070 (BJD94_12790) pilO 2785667..2786257 (+) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  BJD94_RS14075 (BJD94_12795) pilP 2786247..2786759 (+) 513 WP_038940290.1 pilus assembly protein PilP Machinery gene
  BJD94_RS14080 (BJD94_12800) pilQ 2786773..2788557 (+) 1785 WP_017419660.1 type IV pilus secretin PilQ Machinery gene
  BJD94_RS14085 (BJD94_12805) aroK 2788759..2789277 (+) 519 WP_011079357.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 37639.03 Da        Isoelectric Point: 6.2692

>NTDB_id=200822 BJD94_RS14060 WP_103155248.1 2784087..2785094(+) (pilM) [Vibrio vulnificus Env1]
MGKPFVTGIDIGHRSLKAVILKPYGEHYALLGYKEIIIKDGIFSDNHTLNHQEIVNSLKELKKELPLFRYQVSLAVPDSS
VISKMLSIDAELDGREREFAILQAFAHQSPFPVDELSIDYVQLVEETSKTNHSYQVYATKKEVVESRVRVAEEVGLKPVL
VDVHAHSLCNILTLAAERHPDKASYCLLDVGYDTTSLVMSRRNEPPLVKEFAYGVSHLNASIGDDLTSPDAHEKTDIFNI
ELTERVRRQLQLFQSMDSQSKVNGLWICGEGAITPLLSETLQRKLSLECEVLNPLALFEQKTAKRKRRIVDWHHFSVAAG
LAIGGIQWLEDSHAA

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=200822 BJD94_RS14060 WP_103155248.1 2784087..2785094(+) (pilM) [Vibrio vulnificus Env1]
ATGGGTAAACCGTTCGTTACCGGAATTGATATTGGTCATCGTAGCCTCAAGGCTGTCATTCTGAAACCTTATGGAGAGCA
CTACGCCCTGTTAGGGTACAAAGAAATAATCATTAAAGACGGTATTTTCTCAGATAACCACACTCTGAATCATCAAGAAA
TTGTCAATAGCCTGAAAGAACTGAAGAAGGAGTTGCCTCTTTTCCGTTATCAGGTGTCACTGGCGGTACCCGATAGTTCA
GTGATTAGTAAGATGTTATCGATCGATGCGGAGTTGGATGGTCGAGAACGAGAGTTCGCTATCCTTCAGGCGTTTGCACA
TCAGTCGCCATTTCCTGTCGATGAATTATCGATTGATTATGTCCAATTGGTCGAAGAAACCAGCAAAACGAATCATAGCT
ATCAAGTTTACGCCACCAAAAAAGAGGTGGTGGAAAGCCGAGTGCGTGTCGCAGAAGAAGTGGGACTGAAACCCGTGCTG
GTAGATGTACATGCGCATAGCCTGTGCAACATCTTAACGTTAGCGGCAGAGCGCCATCCAGACAAAGCCAGCTATTGCTT
GTTGGATGTGGGTTATGACACCACCTCTTTGGTGATGTCGCGTCGCAACGAACCCCCTTTGGTTAAAGAATTTGCCTATG
GAGTGTCTCACCTCAATGCGTCGATAGGCGACGATCTGACTTCTCCTGATGCTCATGAGAAAACCGACATCTTTAATATA
GAGCTGACAGAGCGAGTTCGTCGGCAATTGCAGTTGTTCCAATCCATGGATAGCCAAAGCAAAGTGAATGGCCTTTGGAT
CTGCGGTGAAGGCGCGATTACGCCGTTGTTGAGTGAAACTTTGCAGAGAAAGCTGAGTTTGGAGTGTGAAGTCCTTAATC
CTCTGGCTCTATTTGAGCAAAAAACGGCGAAAAGAAAGCGCCGTATTGTTGACTGGCACCATTTTTCGGTCGCCGCGGGA
TTAGCGATTGGTGGCATTCAGTGGCTGGAGGATAGCCATGCAGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

53.134

100

0.531
  pilM Vibrio cholerae strain A1552

48.974

100

0.499

Multiple sequence alignment